Intercellular communication in phytohemagglutinin – induced lymphocyte agglutinates

Hülser, Dieter F.; Peters, J. H.

doi:10.1002/eji.1830010618

Cited by 27 publications

(11 citation statements)

References 9 publications

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“…However, gap junctions, which are present between most cell types (12,13), appear to occur in vitro between some activated white blood cells. For example, functional and morphological evidence of gap junctional communication between phytohemagglutinin-activated lymphocytes has been reported (14)(15)(16). In addition, dye coupling was reported between lymphocytes and endothelial cells (17).…”

mentioning

confidence: 99%

Leukocytes express connexin 43 after activation with lipopolysaccharide and appear to form gap junctions with endothelial cells after ischemia-reperfusion.

Jara

Borić

Sáez

1995

Proc. Natl. Acad. Sci. U.S.A.

136

111

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mentioning

confidence: 99%

Leukocytes express connexin 43 after activation with lipopolysaccharide and appear to form gap junctions with endothelial cells after ischemia-reperfusion.

Jara

Borić

Sáez

1995

Proc. Natl. Acad. Sci. U.S.A.

136

111

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“…Re (6,(11)(12)(13)(14)(15). Conversely, in differentiating tissues and after cell transformation of the target cell that electrical and metabolic dissociation by hydrolases, the formation of junctional domains coupling develop (27,28). It is thus tempting to assume that requires the transcription and translation of specific messengers modulation of intercellular junctions results from a specific lifor new junctional proteins (16)(17)(18).…”

Section: Methodsmentioning

confidence: 99%

Junctional modulation in mouse embryonal carcinoma cells by Fab fragments of rabbit anti-embryonal carcinoma cell serum.

Dunia¹,

Nicolas²,

Jakob³

et al. 1979

Proc. Natl. Acad. Sci. U.S.A.

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Mouse embryonal carcinoma P-CC4 cells are connected by extensive gap and tight junctions. When the cells are incubated in a medium containing Fab fragments against embryonal carcinoma F9 cells, they round up and a process of junctional removal is initiated. In particular, gap junctions are internalized and after 30 hr of incubation with the anti-F9 Fab fragments both tight and gap junctions are no longer present at the cell surface; however, the cells are still in contact by means of small attachment sites. Early stages of embryonic differentiation seem to be dependent upon relative position of individual blastomeres within the cleaving embryo and upon formation of microenvironmental domains (1-3). It is not known how positional information becomes operative. It is likely that developmental patterns are set by the acquisition of cell surface adhesion properties and by the assembly-modulation, at the right time, of intercellular junctions controlling both the metabolic cooperation between cells and the traffic of metabolites and ions along the paracellular routes of permeation (1-6). The formation of specific attachment sites and junctions accompanies the compaction of blastomeres at the eight-cell stage, which in turn triggers future segregation of presumptive cell types (2). The assumption that the surface features of the blastomere are crucial for compaction is also supported by the observation that this morphogenetic event in mouse preimplanted embryo may be prevented by the addition of Fab fragments from rabbit antiserum to embryonal carcinoma F9 cells (7,8). Anti-F9 Fab fragments can induce reversible decompaction when added to embryos up to the older morulae stage (8), and can block the metabolic coupling in embryonal carcinoma cell lines that are in mutual cooperation (7-9).The purpose of the present paper is to investigate whether anti-F9 Fab fragments are capable of modulating the junctional assemblies in the PCC4 embryonal carcinoma cell line in which "gap" and "tight" junctions are usually abundant. We have found that the addition of anti-F9 Fab fragments results in the loss of tight junctions and internalization of gap junctions. Further assembly of the same types of junctions is blocked after a 30-hr treatment of the cells with anti-F9 Fab fragments. MATERIALS AND METHODSThe embryonal carcinoma cell line PCC4/AzaR1 has been described (10). Rabbit anti-F9 serum, rabbit antiserum against embryonic mouse liver cells, and Fab preparations were produced according to the techniques described (8).Incubation with Anti-F9 Fab Fragments and Control Experiments. PCC4 cells growing in Dulbecco's modified Eagle's medium containing 15% fetal calf serum were suspended in phosphate-buffered saline. After dissociation, the cells were plated on Costar cluster plates in the same culture medium and allowed to grow for either 5 hr or 24 hr to the same final cell density. The anti-F9 Fab fragments (final concentration of 220 ,ug/ml) were added at these times, and the cells were allowed to grow in the presence of...

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“…As early at 1971, it was appreciated that establishment of gap junctional communication may be a hallmark of lymphocyte activation [41]. Hulser and Peters measured electrical contact between bovine lymphocytes stimulated with the non-specific mitogen phytohemagglutinin.…”

Section: Connexin43 In T-lymphocyte Activation and The Immunological mentioning

confidence: 99%

Connexins and pannexins in the immune system and lymphatic organs

Glass

Snyder

Taffet

2015

Cell. Mol. Life Sci.

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Connexin43 and pannexin1 are found in immune cells. While gap junctional communication has been demonstrated between immune cells, hemichannels have been implicated in many cellular functions. Among the functions involved as being connexin dependent and pannexin dependent are cell migration, phagocytosis, antigen presentation, T-cell reactivity and B-cell responses. Surprisingly, many of these connexin-related and pannexin-related functions are not recapitulated in in vivo models. This is leading to a reevaluation of the role of these proteins in immune function.

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Intercellular communication in phytohemagglutinin – induced lymphocyte agglutinates

Abstract: Intercellular communication, as indicated by ion flow between cells, was measured with micro‐glass‐electrodes in lymphocytes immediately after addition of phytohemagglutinin.

Cited by 27 publications

References 9 publications

Leukocytes express connexin 43 after activation with lipopolysaccharide and appear to form gap junctions with endothelial cells after ischemia-reperfusion.

Leukocytes express connexin 43 after activation with lipopolysaccharide and appear to form gap junctions with endothelial cells after ischemia-reperfusion.

Junctional modulation in mouse embryonal carcinoma cells by Fab fragments of rabbit anti-embryonal carcinoma cell serum.

Connexins and pannexins in the immune system and lymphatic organs

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