Interconnected collagen porous scaffolds prepared with sacrificial PLGA sponge templates for cartilage tissue engineering

Xie, Yan; Lee, Kyubae; Wang, Xiuhui; Yoshitomi, Toru; Naoki, Katsuhiko; Yang, Yingnan; Chen, Guoping

doi:10.1039/d1tb01559a

Cited by 34 publications

(24 citation statements)

References 46 publications

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“…Cells are surrounded with their specific microenvironments in vivo. The cellular microenvironment plays an important role on cell functions [ 29 , 30 , 31 , 32 , 33 , 34 ]. Not only are normal somatic cells and stem cells affected by their microenvironments, but cancer cells also communicate and exchange various signals with their tumor microenvironments [ 4 , 5 , 6 , 7 ].…”

Section: Resultsmentioning

confidence: 99%

Morphological Dependence of Breast Cancer Cell Responses to Doxorubicin on Micropatterned Surfaces

et al. 2022

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Cell morphology has been widely investigated for its influence on the functions of normal cells. However, the influence of cell morphology on cancer cell resistance to anti-cancer drugs remains unclear. In this study, micropatterned surfaces were prepared and used to control the spreading area and elongation of human breast cancer cell line. The influences of cell adhesion area and elongation on resistance to doxorubicin were investigated. The percentage of apoptotic breast cancer cells decreased with cell spreading area, while did not change with cell elongation. Large breast cancer cells had higher resistance to doxorubicin, better assembled actin filaments, higher DNA synthesis activity and higher expression of P-glycoprotein than small breast cancer cells. The results suggested that the morphology of breast cancer cells could affect their resistance to doxorubicin. The influence was correlated with cytoskeletal organization, DNA synthesis activity and P-glycoprotein expression.

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Section: Resultsmentioning

confidence: 99%

Morphological Dependence of Breast Cancer Cell Responses to Doxorubicin on Micropatterned Surfaces

et al. 2022

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“…Interconnected collagen porous scaffolds were prepared by a template method [ 51 ]. First, porous sponge templates of PLGA were used with a solvent casting/particulate leaching method.…”

Section: Methodsmentioning

confidence: 99%

Stepwise Proliferation and Chondrogenic Differentiation of Mesenchymal Stem Cells in Collagen Sponges under Different Microenvironments

Zheng

Xie

Yoshitomi

et al. 2022

IJMS

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Biomimetic microenvironments are important for controlling stem cell functions. In this study, different microenvironmental conditions were investigated for the stepwise control of proliferation and chondrogenic differentiation of human bone-marrow-derived mesenchymal stem cells (hMSCs). The hMSCs were first cultured in collagen porous sponges and then embedded with or without collagen hydrogels for continual culture under different culture conditions. The different influences of collagen sponges, collagen hydrogels, and induction factors were investigated. The collagen sponges were beneficial for cell proliferation. The collagen sponges also promoted chondrogenic differentiation during culture in chondrogenic medium, which was superior to the effect of collagen sponges embedded with hydrogels without loading of induction factors. However, collagen sponges embedded with collagen hydrogels and loaded with induction factors had the same level of promotive effect on chondrogenic differentiation as collagen sponges during in vitro culture in chondrogenic medium and showed the highest promotive effect during in vivo subcutaneous implantation. The combination of collagen sponges with collagen hydrogels and induction factors could provide a platform for cell proliferation at an early stage and subsequent chondrogenic differentiation at a late stage. The results provide useful information for the chondrogenic differentiation of stem cells and cartilage tissue engineering.

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“…1–3 Collagen porous scaffolds have been applied for tissue engineering of cartilage, meniscus, bone, ligament, nerve, skin, and related structures. 4–6 Collagen can be used alone or in combination with other biomaterials, such as synthetic polymers, ceramics and other extracellular matrix components. 7,8…”

Section: Introductionmentioning

confidence: 99%

Preparation of mesh-like collagen scaffolds for tissue engineering

et al. 2022

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Interconnected collagen porous scaffolds prepared with sacrificial PLGA sponge templates for cartilage tissue engineering

Abstract: Interconnected pore structures of scaffolds are essential to control cell functions for cartilage tissue engineering. In this study, collagen scaffolds with interconnected pore structures were prepared using poly(D,L-lactide-co-glycolide) (PLGA) sponges...

Cited by 34 publications

References 46 publications

Morphological Dependence of Breast Cancer Cell Responses to Doxorubicin on Micropatterned Surfaces

Morphological Dependence of Breast Cancer Cell Responses to Doxorubicin on Micropatterned Surfaces

Stepwise Proliferation and Chondrogenic Differentiation of Mesenchymal Stem Cells in Collagen Sponges under Different Microenvironments

Preparation of mesh-like collagen scaffolds for tissue engineering

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