Although there is abundant research focusing on estimating the level of returns on stock market, there is a lack of studies examining the comparison of stock return movements for short-term and long-term investment in the Asian stock market. The present study examines return on investment of different holding periods among selected stock markets in Asia. Based on the trading performance of key indices and market capitalization value, Korean Stock Exchange (KRE), Shanghai Stock Exchange (SSE), Hong Kong Stock Exchange (HKSE), and Taiwan Stock Exchange (TWSE) were selected for this study. In assessing the comparative output of the case stock markets, this study used ANOVA and post-hoc analysis to process time series data of daily, monthly, quarterly, semiannually and annually return of investments of indices of the case stock markets. The results of this study showed that there are significant differences in return on investment among the case stock markets in the given holding periods. While a country might have a higher return in short-term investment, it may not necessarily be suitable for long-term investment as evidenced by their performance from 2001 to 2012.