Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides

Misson, Mailin; Jin, Bo; Dai, Sheng; Zhang, Hu

doi:10.3390/catal10010081

Cited by 9 publications

(5 citation statements)

References 46 publications

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“…A direct comparison with literature data is not possible, but in a study that was performed in a continuous packed bed reactor with β-galactosidase immobilized on double-layered hydrophilic polymer-coated magnetic nanoparticles, 100% conversion was achieved [15]. Comparison of lactose bioconversion has shown that in different reactors using different support materials for β-galactosidase immobilization, lactose conversion rates were between 38% and 88% [33].…”

Section: Hydrolysis Of Lactosementioning

confidence: 99%

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The Immobilization of β-Galactosidase on Glass Fiber Rolls

Pečar,

Zečević,

Goršek

2023

Catalysts

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The usability of glass fibers as immobilization support with a porous open structure was investigated. We developed a method to immobilize the enzyme β-galactosidase on special glass fiber rolls. The new method is simple, non-expensive and industrially applicable. Glutaraldehyde was used as a non-specific cross-linking agent for the covalent binding of β-galactosidase on modified glass fibers. The efficiency of immobilization was tested with the known hydrolysis of lactose. All experiments were performed in a continuous laboratory reactor. The influence of the reaction temperature (20, 25 and 30 °C), the substrate flow rate (1, 2 and 3 mL/min) and the pH of the reaction medium (6, 7 and 8) on the conversion was investigated. The reaction efficiency was monitored by measuring the glucose concentration with a spectrophotometer. High immobilization efficiency, enzyme activity and stability were obtained. The optimal reaction temperature, substrate flow rate and pH were found. The activity and stability of the enzyme entrapped on the glass fiber rolls remained almost unchanged during reuse, which is promising for potential industrial applications.

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Section: Hydrolysis Of Lactosementioning

confidence: 99%

“…Catalysts 2023, 13, x FOR PEER REVIEW 7 of 12 using different support materials for β-galactosidase immobilization, lactose conversion rates were between 38% and 88% [33].…”

Section: Hydrolysis Of Lactosementioning

confidence: 99%

The Immobilization of β-Galactosidase on Glass Fiber Rolls

Pečar,

Zečević,

Goršek

2023

Catalysts

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“…Electrospinning is applied as a versatile, potentially high throughput, and powerful means of continuously preparing desirable fibrous membranes, which are considered good carriers for enzyme immobilization because of their high porosity, interconnectivity, and variable spinning materials. − Notably, the nanofiber membranes can retain their morphological structures well through hydrolysis and can be surface functionalized with stimuli-responsive polymer chains (Figure ).…”

Section: Strategies For Generating Sr-ppmsmentioning

confidence: 99%

Design of Switchable Enzyme Carriers Based on Stimuli-Responsive Porous Polymer Membranes for Bioapplications

Qiao

2021

ACS Appl. Bio Mater.

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Design of efficient enzyme carriers, where enzymes are conjugated to supports, has become an attractive research avenue. Immobilized enzymes are advantageous for practical applications because of their convenience in handling, ease of separation, and good reusability. However, the main challenge is that these traditional enzyme carriers are unable to regulate the enzymolysis efficiency or to protect the enzymes from proteolytic degradation, which restricts their effectiveness of enzymes in bioapplications. Enlightened by the stimuli-responsive channels in the natural cell membranes, conjugation of the enzymes within flat-sheet stimuli-responsive porous polymer membranes (SR-PPMs) as artificial cell membranes is an efficient strategy for circumventing this challenge. Controlled by the external stimuli, the multifunctional polymer chains, which are incorporated within the membranes and attached to the enzyme, change their structures to defend the enzyme from the external environmental disturbances and degradation by proteinases. Specifically, smart SR-PPM enzyme carriers (SR-PPMECs) not only permit convective substrate transfer through the accessible porous network, dramatically improving enzymolysis efficiency due to the adjustable pore sizes and the confinement effect, but they also act as molecular switches for regulating its permeability and selectivity. In this review, the concept of SR-PPMECs is presented. It covers the latest developments in design strategies of flat-sheet SR-PPFMs, fabrication protocols of SR-PPFMECs, strategies for the regulation of enzymolysis efficiency, and their cutting-edge bioapplications.

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“…Fathi et al (2022) reported that substrate concentration, temperature and pH could influence enzyme activity. β-galactosidase, an enzyme of significant industrial importance, has attracted attention in the industry for its pivotal role in catalyzing the breakdown of lactose molecules, leading to the creation of enzyme-galactosyl ISSN (print): 1823-8262, ISSN (online): 2231-7538 complexes (Misson et al, 2020). These complexes then proceed down either the hydrolysis or transgalactosylation pathways.…”

Section: Introductionmentioning

confidence: 99%

Enhancing stability and activity of β-galactosidase from Kluyveromyces lactis through immobilization on polymethacrylate monolith and optimisation using response surface methodology

Dahari, R. F.,

Misson, M.,

Ongkudon, C. M.

et al. 2023

MJM

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Aims: This study investigates the potential of porous polymethacrylate monoliths as enzyme support materials for largescale enzyme commercialization. Methodology and results: It focuses on their preparation and various immobilization techniques, such as adsorption, covalent-binding and cross-linking, specifically applied to β-galactosidase for bioprocess applications. The research assesses immobilization performance, operational stability, reusability and optimization using response surface methodology (RSM). The results reveal that covalent-binding exhibited the highest enzyme activity recovery, while cross-linking showed superior performance at lower enzyme concentrations but decreased at higher concentrations. Covalent-bound enzymes demonstrated reusability for up to four cycles, with optimal pH ranging between 7 and 8 and optimal temperature ranging between 30 °C and 40 °C. Furthermore, RSM optimization highlighted the significant influence of substrate concentration on enzyme activity, with a reliable model (R 2 = 0.9163) and adequate precision (S/N = 13.1409). Conclusion, significance and impact of study: Overall, this study provides valuable guidelines for effectively employing porous monoliths in large-scale industrial bioprocessing, offering potential cost-saving benefits and enhanced efficiency in enzyme commercialization.

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Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides

Cited by 9 publications

References 46 publications

The Immobilization of β-Galactosidase on Glass Fiber Rolls

The Immobilization of β-Galactosidase on Glass Fiber Rolls

Design of Switchable Enzyme Carriers Based on Stimuli-Responsive Porous Polymer Membranes for Bioapplications

Enhancing stability and activity of β-galactosidase from Kluyveromyces lactis through immobilization on polymethacrylate monolith and optimisation using response surface methodology

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