Interfacial capacitance immunosensing using interdigitated electrodes: the effect of insulation/immobilization chemistry

Castiello, F. Rafael; Porter, James M.; Modarres, Paresa; Tabrizian, Maryam

doi:10.1039/c9cp02129a

Cited by 25 publications

(40 citation statements)

References 42 publications

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“…For example, decreasing the spacing between interdigitated electrodes will reduce the influence of

. Also, electrodes with a thin conformal coating of insulative material would enhance the stability and sensitivity of the capacitance measurement ( Castiello et al, 2019 ). Future tests will determine if measurements acquired at other frequencies and/or voltages ( i.e., other than 10 kHz, 22 mV) will further improve platform performance.…”

Section: Resultsmentioning

confidence: 99%

“…The performance of capacitive immunosensors can be adjusted by modifying the electrode surface area, insulation (coating) layer, and the electrode and coating material. Highly sensitive sensors can be achieved by making thin conformal coating layer which ensure stable capacitance changes in response to probe/target binding ( Castiello et al, 2019 ). Several studies reported the use of capacitance immunosensors for detection of pathogens ( Cesewski and Johnson, 2020 , Hanna et al, 2006 , Luka et al, 2019 , Teeparuksapun et al, 2012 , Wang et al, 2019 , Wang et al, 2017 ), thus demonstrating that it can be used as a reliable label-free sensing method.…”

Section: Introductionmentioning

confidence: 99%

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Rapid detection of SARS-CoV-2 antibodies using electrochemical impedance-based detector

Rashed

Kopechek

Priddy

et al. 2021

Biosensors and Bioelectronics

161

122

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Coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was classified as a pandemic by the World Health Organization and has caused over 550,000 deaths worldwide as of July 2020. Accurate and scalable point-of-care devices would increase screening, diagnosis, and monitoring of COVID-19 patients. Here, we demonstrate rapid label-free electrochemical detection of SARS-CoV-2 antibodies using a commercially available impedance sensing platform. A 16-well plate containing sensing electrodes was pre-coated with receptor binding domain (RBD) of SARS-CoV-2 spike protein, and subsequently tested with samples of anti-SARS-CoV-2 monoclonal antibody CR3022 (0.1 g/ml, 1.0 g/ml, 10 g/ml). Subsequent blinded testing was performed on six serum specimens taken from COVID-19 and non-COVID-19 patients (1:100 dilution factor). The platform was able to differentiate spikes in impedance measurements from a negative control (1% milk solution) for all CR3022 samples. Further, successful differentiation and detection of all positive clinical samples from negative control was achieved. Measured impedance values were consistent when compared to standard ELISA test results showing a strong correlation between them (R ). Detection occurs in less than five minutes and the well-based platform provides a simplified and familiar testing interface that can be readily adaptable for use in clinical settings.

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“…For example, decreasing the spacing between interdigitated electrodes will reduce the influence of

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rapid detection of SARS-CoV-2 antibodies using electrochemical impedance-based detector

Rashed

Kopechek

Priddy

et al. 2021

Biosensors and Bioelectronics

161

122

View full text Add to dashboard Cite

show abstract

“…For example, decreasing the spacing between interdigitated electrodes will reduce the influence of Rsus. Also, electrodes with a thin conformal coating of insulative material would enhance the stability and sensitivity of the capacitance measurement (Castiello et al (2019)). Future tests will determine if measurements acquired at other frequencies and/or voltages ( i.e ., other than 10 kHz, 22 mV) will further improve platform performance.…”

Section: Resultsmentioning

confidence: 99%

“…The performance of capacitive im-munosensors can be adjusted by modifying the electrode surface area, insulation (coating) layer, and the electrode and coating material. Highly sensitive sensors can be achieved by making thin conformal coating layer which ensure stable capacitance changes in response to probe/target binding (Castiello et al (2019)). Several studies reported the use of capacitance immunosensors for detection of pathogens (Cesewski and Johnson (2020); Hanna et al (2006); Luka et al (2019); Teeparuksapun et al (2012); Wang et al (2019, 2017)), thus demonstrating that it can be used as a reliable label-free sensing method.…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection of SARS-CoV-2 Antibodies Using Electrochemical Impedance-Based Detector

Rashed

Kopechek

Priddy

et al. 2020

Preprint

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Emerging novel human contagious viruses and pathogens put humans at risk of hospitalization and possibly death due to the unavailability of vaccines and drugs which may take years to develop. Coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was classified as a pandemic by theWorld Health Organization and has caused over 550,000 deaths worldwide as of July 2020. Accurate and scalable point-of-care devices would increase screening, diagnosis, and monitoringof COVID-19 patients. Here, we demonstrate rapid label-free electrochemical detection of SARS-CoV-2 antibodies using a commercially available impedance sensing platform. A 16-well plate containing sensing electrodes was pre-coated with receptor binding domain (RBD) of SARS-CoV-2 spike protein, and subsequently tested with samples of anti-SARS-CoV-2 monoclonal antibody CR3022 (0.1 μg/ml, 1.0 μg/ml, 10 μg/ml). Subsequent blinded testing was performed on six serum specimens taken from COVID-19 and non-COVID-19 patients (1:100 dilution factor). The platformwas able to differentiate spikes in impedance measurements from a negative control (1~ milk solution) for all CR3022 samples. Further, successful differentiation and detection of all positive clinical samples from negative control was achieved. Measured impedance values were consistent when compared to standard ELISA test results showing a strong correlation between them (R2 = 0:9). Detection occurs in less than five minutes and the well-based platform provides a simplified and familiar testing interface that can be readily adaptable for use in clinical settings.

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“…As a result, an increase in charge transfer resistance (Rct) occurs. Regarding capacitive biosensors, antibodies [30][31][32][33], aptamers [34,35], and affimers [36] have been used as recognition elements. Capacitive biosensors rely on changes occurring within the electrical double layer (EDL), where the thickness is changed according to binding of a target to a recognition element on the electrode.…”

Section: Measurement Of Fructosyl Lysine and Ga With An Ide Enzyme Sementioning

confidence: 99%

Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement

et al. 2021

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Glycated albumin (GA) is an important glycemic control marker for diabetes mellitus. This study aimed to develop a highly sensitive disposable enzyme sensor strip for GA measurement by using an interdigitated electrode (IDE) as an electrode platform. The superior characteristics of IDE were demonstrated using one microelectrode of the IDE pair as the working electrode (WE) and the other as the counter electrode, and by measuring ferrocyanide/ferricyanide redox couple. The oxidation current was immediately reached at the steady state when the oxidation potential was applied to the WE. Then, an IDE enzyme sensor strip for GA measurement was prepared. The measurement of fructosyl lysine, the protease digestion product of GA, exhibited a high, steady current immediately after potential application, revealing the highly reproducible measurement. The sensitivity (2.8 nA µM−1) and the limit of detection (1.2 µM) obtained with IDE enzyme sensor strip were superior compared with our previously reported sensor using screen printed electrode. Two GA samples, 15 or 30% GA, corresponding to healthy and diabetic levels, respectively, were measured after protease digestion with high resolution. This study demonstrated that the application of an IDE will realize the development of highly sensitive disposable-type amperometric enzyme sensors with high reproducibility.

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Interfacial capacitance immunosensing using interdigitated electrodes: the effect of insulation/immobilization chemistry

Abstract: With the aim of improving the reproducibility of capacitive immunosensors, we performed a comparative study of four different insulating/immobilization chemistries.

Cited by 25 publications

References 42 publications

Rapid detection of SARS-CoV-2 antibodies using electrochemical impedance-based detector

Rapid detection of SARS-CoV-2 antibodies using electrochemical impedance-based detector

Rapid Detection of SARS-CoV-2 Antibodies Using Electrochemical Impedance-Based Detector

Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement

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