Interfacial electron transfer of glucose oxidase on poly(glutamic acid)-modified glassy carbon electrode and glucose sensing

Zhou, Xuechou; Tan, Bingcan; Zheng, Xinyu; Kong, Dexian; Li, Qinglu

doi:10.1016/j.ab.2015.08.007

Cited by 19 publications

(6 citation statements)

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“…An appropriate balance between the enzymatic and electrochemical activities is vital for DET glucose biosensors, and more innovation is still necessary to reach that balance and a commercially viable product. Various attempts to overcome the long electron-tunneling distance were made to demonstrate the feasibility of a DET glucose sensor (Liang et al, 2015, Terse-Thakoor et al, 2015, Zhang et al, 2015c, Zhou et al, 2015, Blaik et al, 2016). In these reports, the improved sensing performance by the direct electron transfer has been realized by incorporating the enzyme with nanomaterials, which play an important role in the enzyme immobilization, owing to nanomaterial’s highly specific surface area, good biocompatibility, stability, and facilitating electron transfer (Willner et al, 2006).…”

Section: The Development Of Glucose Metermentioning

confidence: 99%

Transforming the blood glucose meter into a general healthcare meter for in vitro diagnostics in mobile health

Lan

Zhang

2016

Biotechnology Advances

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Recent advances in mobile network and smartphones have provided an enormous opportunity for transforming in vitro diagnostics (IVD) from central labs to home or other points of care (POC). A major challenge to achieving the goal is a long time and high costs associated with developing POC IVD devices in mobile Health (mHealth). Instead of developing a new POC device for every new IVD target, we and others are taking advantage of decades of research, development, engineering and continuous improvement of the blood glucose meter (BGM), including those already integrated with smartphones, and transforming the BGM into a general healthcare meter for POC IVDs of a wide range of biomarkers, therapeutic drugs and other analytical targets. In this review, we summarize methods to transduce and amplify selective binding of targets by antibodies, DNA/RNA aptamers, DNAzyme/ribozymes and protein enzymes into signals such as glucose or NADH that can be measured by commercially available BGM, making it possible to adapt many clinical assays performed in central labs, such as immunoassays, aptamer/DNAzyme assays, molecular diagnostic assays, and enzymatic activity assays onto BGM platform for quantification of non-glucose targets for a wide variety of IVDs in mHealth.

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Section: The Development Of Glucose Metermentioning

confidence: 99%

Transforming the blood glucose meter into a general healthcare meter for in vitro diagnostics in mobile health

Lan

Zhang

2016

Biotechnology Advances

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“…In Table , important analytical parameters are compared with other glucose sensors including carbon material. The result explains that FMPS‐Gly microsphere/CPE biosensor shows quite comparable or better performance than other modified electrodes .…”

Section: Resultsmentioning

confidence: 79%

Glucose biosensor based on immobilization of glucose oxidase on a carbon paste electrode modified with microsphere‐attached l‐glycine

Dönmez

Arslan

Sarı

et al. 2017

Biotech and App Biochem

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In the present study, a novel biosensor that is sensitive to glucose was prepared using the microspheres modified with (4-formyl-3-methoxyphenoxymethyl)polystyrene (FMPS) with l-glycine. Polymeric microspheres having Schiff bases were prepared from FMPS using the glycine condensation method. Glucose oxidase enzyme was immobilized onto modified carbon paste electrode by cross-linking with glutaraldehyde. Oxidation of enzymatically produced H O (+0.5 V vs. Ag/AgCl) was used for determination of glucose. Optimal temperature and pH were found as 50 °C and 8.0, respectively. The glucose biosensor showed a linear working range from 5.0 × 10 to 1.0 × 10 M, R = 0.999. Storage and operational stability of the biosensor were also investigated. The biosensor gave perfect reproducible results after 20 measurements with 3.3% relative standard deviation. It also had good storage stability.

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“…For this reason, reliable and accurate glucose detection in blood is of critical importance. Additionally, glucose sensing is important in food and textile industries, as well as environmental monitoring. , Usually, glucose sensing relies on the use of glucose oxidase (GOx), which catalyzes glucose in the presence of O 2 to afford gluconic acid and H 2 O 2 . , The concentration of glucose is then determined by monitoring the number of electrons flowing through the enzyme, or the concentration of the as formed H 2 O 2 is determined quantitatively as an indicator of the amount of glucose present in the solution.…”

Section: Overview Of Carbon-based Voltammetric Biosensorsmentioning

confidence: 99%

“…110,111 Usually, glucose sensing relies on the use of glucose oxidase (GOx), which catalyzes glucose in the presence of O 2 to afford gluconic acid and H 2 O 2 . 112,113 The concentration of glucose is then determined by monitoring the number of electrons flowing through the enzyme, or the concentration of the as formed H 2 O 2 is determined quantitatively as an indicator of the amount of glucose present in the solution.…”

Section: Overview Of Carbon-based Voltammetric Biosensorsmentioning

confidence: 99%

Engineered Carbon-Nanomaterial-Based Electrochemical Sensors for Biomolecules

et al. 2015

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The study of electrochemical behavior of bioactive molecules has become one of the most rapidly developing scientific fields. Biotechnology and biomedical engineering fields have a vested interest in constructing more precise and accurate voltammetric/amperometric biosensors. One rapidly growing area of biosensor design involves incorporation of carbon-based nanomaterials in working electrodes, such as one-dimensional carbon nanotubes, two-dimensional graphene, and graphene oxide. In this review article, we give a brief overview describing the voltammetric techniques and how these techniques are applied in biosensing, as well as the details surrounding important biosensing concepts of sensitivity and limits of detection. Building on these important concepts, we show how the sensitivity and limit of detection can be tuned by including carbon-based nanomaterials in the fabrication of biosensors. The sensing of biomolecules including glucose, dopamine, proteins, enzymes, uric acid, DNA, RNA, and H2O2 traditionally employs enzymes in detection; however, these enzymes denature easily, and as such, enzymeless methods are highly desired. Here we draw an important distinction between enzymeless and enzyme-containing carbon-nanomaterial-based biosensors. The review ends with an outlook of future concepts that can be employed in biosensor fabrication, as well as limitations of already proposed materials and how such sensing can be enhanced. As such, this review can act as a roadmap to guide researchers toward concepts that can be employed in the design of next generation biosensors, while also highlighting the current advancements in the field.

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Interfacial electron transfer of glucose oxidase on poly(glutamic acid)-modified glassy carbon electrode and glucose sensing

Cited by 19 publications

References 50 publications

Transforming the blood glucose meter into a general healthcare meter for in vitro diagnostics in mobile health

Transforming the blood glucose meter into a general healthcare meter for in vitro diagnostics in mobile health

Glucose biosensor based on immobilization of glucose oxidase on a carbon paste electrode modified with microsphere‐attached l‐glycine

Engineered Carbon-Nanomaterial-Based Electrochemical Sensors for Biomolecules

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