Interfacial Nucleic Acid Hybridization Studied by Random Primer 32P Labeling and Liquid-Phase Acoustic Network Analysis

Abstract

Single-strand DNA labeled with 32P by the random primer method has been adsorbed to the surface of palladium electrodes of thickness shear-mode acoustic wave sensors and nylon membranes under various solution conditions. The mass of nucleic acid attached to these substrates has been quantified by scintillation counting and phosphor image analysis. Measurement of the mass on sensors by acoustic wave sensor response obtained in the gas phase does not correlate with the values given by radiochemical labeling. Hybridization of cDNA to the single-strand species on the sensor surface has also been determined by the 32P technique. The added mass has been correlated with real-time changes in the series resonant frequency obtained in situ from acoustic network analysis. The value for the alteration of the frequency is 18 times that predicted by the Sauerbrey expression. This result is ascribed

Indicator Free DNA Hybridization Detection by Impedance Measurement Based on the DNA‐Doped Conducting Polymer Film Formed on the Carbon Nanotube Modified Electrode

“…These techniques present some inconvenience due to their need for time-consuming and low rate of production labeling process. Other DNA biosensor based on surface acoustic wave [7], quartz crystal microbalance (QCM) [8,9], and electrochemical methods [10 ± 16] are also widely reported. Electrochemical techniques are well suited for rapid detection of specific DNA sequences for their high sensitivity, small dimensions, low cost, and compatibility with micro-fabrication technology of transducers.…”

Determination of Cytochrome C with Cellulose – DNA Modified Carbon Paste Electrodes

“…Recent research activities have focused on the design of sequence selective biosensors based on the monitoring of effects caused by hybridization [17 ± 20]. Such approaches commonly include the immobilization of a single-stranded (ss) DNA probe onto optical [18,21], electrochemical [18,19,22], or acoustic transducers [20] to selectively bind to a complementary (target) DNA strand in a sample solution. Another usage of immobilized DNA is to exploit a selective molecular interaction between oligomers, natural, chromosomal, or plasmid DNAs and certain pollutants or drugs.…”

Rheological and interfacial properties of nucleic acid films studied by thickness – shear mode sensor and network analysis

“…An HP4 195A impedance kit was used to make direct measurements of impedance as described previously (20). The values of the equivalent circuit elements of the quartz crystal are calculated internally by the HP 4195A from the measured data.…”

“…We have reported in previous papers the in situ detection of polynucleotide (19) and pPT-2 DNA hybridization (20) at the surface of palladium electrodes sputtered onto underlying gold electrodes of TSM sensors. Hybridization of complementary strands of nucleic acid at the device-liquid interface results in series resonant frequency signals that are significantly higher than expected from the conventional Sauerbrey mass response.…”