Interfacial tension effects of nonionic surfactants in reversed-phase liquid chromatography

Additive effects on surfactant adsorption and ionic solute retention in micellar liquid chromatography

“…Retention Study. At surfactant concentrations below the cmc, micelles do not exist, and, as demonstrated by Knox (29), Deming (30), and our previous work (31), the degree of retention was directly related to the surface charge arising from the adsorbed surfactant. With both the surfactants, the retention of neutral species (toluene and caffeine) slightly decreased when the amount of adsorbed surfactant was increased.…”

Micellar liquid chromatography. Retention study of solutes of various polarities

“…The cmc values for SDS and CTAB are 8 X 3 mol/L and 8 X 10~4 mol/L, respectively. At surfactant concentrations below cmc, micelles do not exist and, as demonstrated by Knox (11) and Deming (12)(13)(14), the degree of retention is directly related to the surface charge arising from the adsorbed surfactant; i.e., the retention of neutral species slightly decreases with increase in the concentration of adsorbed surfactant. When an anionic surfactant is adsorbed onto the stationary phase, the retention of negatively charged solutes falls dramatically, whereas the retention of cationic solutes increases.…”

Method for Recovery and Immunoaffinity Enrichment of Membrane Proteins Illustrated with Metastatic Ovarian Cancer Tissues

“…With the exception of newer acid-cleavable forms [ 21 ], surfactants can produce ionization problems for mass spectrometric analyses, except at very low concentrations [ 22 , 23 ], which are too low to support solubility of membrane proteins. Surfactants also bind to surfaces, significantly altering the behavior of liquid chromatographic media [ 24 ].…”