Interfacial Tension of Phase-Separated Polydisperse Mixed Polymer Solutions

Vis, Mark; Blokhuis, Edgar M.; Erné, Ben H.; Tromp, R. Hans; Lekkerkerker, H. N. W.

doi:10.1021/acs.jpcb.7b09967

Cited by 13 publications

(10 citation statements)

References 36 publications

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“…l c was obtained here by fitting the static interfacial profile to the generalized Young-Laplace equation near a flat wall, as shown in Figure 8. γ cc was found to be 311 µN/m in agreement with values found in the recent literature on water/water interfacial tension in both associative complex coacervates [50][51][52][53][54] and segregative aqueous two phase systems (ATPS) (dextran/PEG [55], dextran/gelatin [56]). A very small amount of energy is then sufficient to create an additional surface between the two coexisting PDADMAC/PANa phases; no ion pair breaking is then necessary but only their redistribution in the case of a neutral complex [57].…”

Section: Interfacial Tension Between the Dilute And Concentrated Coacervate Phasesupporting

confidence: 88%

Interfacial Behavior of Solid- and Liquid-like Polyelectrolyte Complexes as a Function of Charge Stoichiometry

Fauquignon

Haddou

et al. 2021

Polymers

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We systematically investigate in this work the surface activity of polyelectrolyte complex (PECs) suspensions as a function of the molar charge ratio Z (= [-]/[+]) from two model systems: the weakly and strongly interacting poly (diallyldimethylammonium chloride)/poly (acrylic acid sodium salt) (PDADMAC/PANa) and poly (diallyldimethylammonium chloride)/poly (sodium 4- styrenesulfonate) (PDADMAC/PSSNa) pairs, respectively. For both systems, the PEC surface tension decreases as the system approaches charge stoichiometry (Z = 1) whenever the complexation occurs in the presence of excess PDADMAC (Z < 1) or excess polyanion (Z > 1) consistent with an increased level of charge neutralization of PEs forming increasingly hydrophobic and neutral surface-active species. The behavior at stoichiometry (Z = 1) is also particularly informative about the physical nature of the complexes. The PDADMAC/PANa system undergoes a liquid–liquid phase transition through the formation of coacervate microdroplets in equilibrium with macroions remaining in solution. In the PDADMAC/PSSNa system, the surface tension of the supernatant was close to that of pure water, suggesting that the PSSNa-based complexes have completely sedimented, consistent with a complete liquid–solid phase separation of an out-of-equilibrium system. Besides, the high sensitivity of surface tension measurements, which can detect the presence of trace amounts of aggregates and other precursors in the supernatant, allows for very accurate determination of the exact charge stoichiometry of the complexes. Finally, the very low water/water interfacial tension that develops between the dilute phase and the denser coacervate phase in the PDADAMAC/PANa system was measured using the generalized Young–Laplace method to complete the full characterization of both systems. The overall study showed that simple surface tension measurements can be a very sensitive tool to characterize, discriminate, and better understand the formation mechanism of the different structures encountered during the formation of PECs.

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Section: Interfacial Tension Between the Dilute And Concentrated Coacervate Phasesupporting

confidence: 88%

Interfacial Behavior of Solid- and Liquid-like Polyelectrolyte Complexes as a Function of Charge Stoichiometry

Fauquignon

Haddou

et al. 2021

Polymers

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“…2A we plot the surface tension (γ) between the condensate (protein-rich) and protein-poor liquid phases as a function of temperature (renormalized by the highest critical temperature of the protein set, T c of h-TDP-43). An advantage of computer simulations is that γ between two coexisting fluid phases (or between a fluid and a vapour one) can be easily computed, as explained in SIV , compared to more challenging approaches (i.e., based on the tie-line width of the phase diagrams) as required in experimental setups 53,141 . We find that the conformational difference in the 40-residue helical region of the TDP-43-PLD terminal domain has significant consequences on the droplet surface tension of TDP-43.…”

Section: B Structural and Interfacial Properties Of The Condensates Without Rnamentioning

confidence: 99%

RNA modulation of transport properties and stability in phase separated condensates

Tejedor

Garaizar

Ramı́rez

et al. 2021

Preprint

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One of the key mechanisms employed by cells to control their spatiotemporal organization is the formation and dissolution of phase-separated condensates. Such balance between condensate assembly and disassembly can be critically regulated by the presence of RNA. In this work, we use a novel chemically accurate coarse-grained model for proteins and RNA to unravel the impact of poly-uridine RNA in modulating the protein mobility and stability within different biomolecular condensates. We explore the behavior of FUS, hnRNPA1 and TDP-43 proteins along their corresponding prion-like domains from absence to moderately high RNA concentration. By characterising the phase diagram, key molecular interactions, surface tension and viscoelastic properties, we report a dual RNA-induced behavior: On the one hand, poly-uridine enhances phase separation at low concentration, whilst at high concentration, it inhibits the ability of proteins to self-assemble. On the other hand, as a consequence of such stability modulation, the transport properties of proteins within the condensates are significantly enhanced at moderately high RNA concentration, as long as the length of poly-uridine strands is comparable or moderately shorter than those of the proteins. On the whole, our work elucidates the different routes by which RNA can regulate phase separation and condensate dynamics, as well as the subsequent aberrant rigidification implicated in the emergence of various neuropathologies and age-related diseases.

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“…3−6 Owing to the similar physic-chemical properties of the two immiscible aqueous phases in the ATPS, the interfacial tensions between them was usually ultralow. 7 The ultralow interfacial tensions in the polymer-based ATPS provide a mild environment for the transport of biological molecules, such as proteins, 8 nucleic acids, 9 and cellular materials, 10,11 across the interface and also the feasibility for separation of targets with extremely similar physic-chemical properties. 12 As an economic and environmentally friendly extraction system, polymer-based ATPS exhibited a significant potential in recovery of metal ions from various complicated industrial wastewaters.…”

Section: ■ Introductionmentioning

confidence: 99%

Role of Hydrophobic Interaction in Driving the Partitioning of Metal Ions in a PEG-Based Aqueous Two-Phase System

Sun

Huang

Lin

et al. 2018

Ind. Eng. Chem. Res.

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PEG-based aqueous two-phase systems (ATPS) for extraction and removal of metal ions from industrial wastewaters is a promising strategy to replace traditional organic-aqueous two phase systems, due to its environmentally friendly advantages and excellent separation efficiency from controllable phase structure and physicochemical properties. However, controversy still remains about the partitioning mechanism of metal ions into the PEG-rich phase in ATPS. The present contribution revealed that a specific hydrophobic interaction was a main driving force for the transferring of Cr(VI) ions into the PEG-rich phase in ATPS. The properties of aqueous media around the PEG molecules and Cr(VI) ions also played a crucial role in the partitioning of Cr(VI) ions in the PEG-base ATPS. The PEG-rich phase in the ATPS exhibited a specific affinity toward poorly hydrated HCrO4 – ions, compared to the strongly hydrated CrO4 2– ions. The difference in the properties of aqueous media in the coexisting liquid phases was response for the partitioning of Cr(VI) ions in the PEG-base ATPS. It was confirmed by combined application of UV–vis spectroscopy and molecular dynamics simulations. The present work provides a new understanding into the microscopic mechanism of the partitioning of metal ions in the polymer-based ATPS.

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Interfacial Tension of Phase-Separated Polydisperse Mixed Polymer Solutions

Cited by 13 publications

References 36 publications

Interfacial Behavior of Solid- and Liquid-like Polyelectrolyte Complexes as a Function of Charge Stoichiometry

Interfacial Behavior of Solid- and Liquid-like Polyelectrolyte Complexes as a Function of Charge Stoichiometry

RNA modulation of transport properties and stability in phase separated condensates

Role of Hydrophobic Interaction in Driving the Partitioning of Metal Ions in a PEG-Based Aqueous Two-Phase System

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