Interfacially Polymerized Particles with Heterostructured Nanopores for Glycopeptide Separation

Abstract

Porous polymer materials are extensively used for biomolecule separation. However, conventional homogeneous porous polymer materials cannot efficiently separate specific low-abundance biomolecules from complex samples. Here, particles fabricated by emulsion interfacial polymerization featuring heterostructured nanopores with tunable size are reported, which can be used to realize low-abundance glycopeptide (GP) separation from complex biofluids. The heterostructured surface inside the nanopores allows solvent-dependent local adsorption of biomolecules onto hydrophilic or hydrophobic regions. Low-abundance hydrophilic GPs in complex biofluids can be efficiently separated via the hydrophilic region of nanopores in low-polarity solvent after the hydrophobic region removes high-abundance hydrophobic proteins and non-glycopeptides in high-polarity solvent. It is expected that these particles with heterostructured nanopores can be used for separation of nucleic acids, saccharides, and proteins, and downstream clinical diagnosis.

Grenzflächenpolymerisation: Von der Chemie zu funktionellen Materialien

“…Mittels Grenzflächenpolyadditionk çnnen funktionelle Materialien wie Janus-Partikel, heterostruktu-rierte Partikel und Janus-Filme hergestellt und zur Stofftrennung und Sensorik verwendet werden. [8,22,91] Die Monomere in der Polyaddition sind zahlreich und programmierbar,d aher werden in Kombination mit der Grenzflächenpolyaddition immer mehr Polymermaterialien erzeugt und verschiedene Funktionen weiter entwickelt.…”

Nanomaterials in Proteomics

“…Finally, 356 glycopeptides mapped to 119 glycoproteins were identified from 0.35 µL of human serum in three runs. More interestingly, Song's groups fabricated a string of porous heterostructured nanomaterials with hydrophilicity–hydrophobicity via the emulsion interfacial polymerization method (displayed in Figure A) . Thereinto, two poly(styrene‐divinyl benzene)–polyacrylamide (PSDVB–PAM) nanoparticles with different pore size (3 and 33 nm) were applied to capture glycopeptides according to the work flow displayed in Figure B.…”

“…The oil‐in‐water emulsion includes hydrophobic monomers (St and DVB) in oil and hydrophilic monomers (AM) in water; B) glycopeptides separation using the two kinds of nanoparticles with different pore size. Reproduced with permission . Copyright 2018, Wiley‐VCH.…”

pH‐Regulated Heterostructure Porous Particles Enable Similarly Sized Protein Separation

“…Alternatively, a block copolymer self‐assembly strategy was established for fabricating polystyrene‐ b ‐poly(acrylic acid) (PS‐ b ‐PAA) porous particles with triply periodic continuous minimal surfaces . Recently, we proposed a heterostructured model at the nanoscale to avoid surface defects, thus achieving effective separation of trace glycopeptides from complex biological samples . This model may provide an opportunity to produce unique functional porous particles for the separation of similarly sized proteins.…”

“…First, the successful synthesis of HESP demonstrates that interfacial polymerization can be performed with a double emulsion system, not only with a single emulsion system, thus greatly enriching the family of porous particles for protein separation. Second, the tunable surface chemistry of the HESP will provide versatile candidates for the highly effective separation of various specific proteins and other biomacromolecules . Some related projects are ongoing in our laboratory.…”