Interfacing microbore and capillary liquid chromatography to continuous-flow fast atom bombardment mass spectrometry for the analysis of glycopeptides

Coutant, John E.; Chen, Teng‐Man; Ackermann, Bradley L.

doi:10.1016/s0378-4347(00)83833-5

Cited by 33 publications

(10 citation statements)

References 14 publications

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“…This matrix generally consists of an aqueous glycerol solution. The matrix solution can be either coaxially added to the post-column effluent [243,244] via an addition tee [245,246]. Alternatively, the glycerol matrix can be added to the mobile phase [247,248].…”

Section: Continuous-flow Fast Atom Bombardmentmentioning

confidence: 99%

Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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This review discusses many different aspects of microcolumn liquid chromatography (LC) and reflects the areas of microcolumn LC research interest over the past decades. A brief theoretical discussion on a number of major issues, such as column characterisation, chromatographic dilution effects and extracolumn band broadening in microcolumn LC is given. Recent progress in column technology and the demands and developments of instrumentation and accessories for microcolumn LC are also reviewed. Besides that, the developments in a large number of established and also more recent detection techniques for microcolumn LC are also discussed. The potential of hyphenation of microcolumn LC with other techniques, more particularly of multidimensional chromatography and microcolumn LC coupled to mass spectrometry is reviewed. Finally, the perspective of microcolumn LC separation methods is stressed by a number of relevant applications.

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Section: Continuous-flow Fast Atom Bombardmentmentioning

confidence: 99%

Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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“…With compatible flow rates, the coupling of capillary LC to nano-or microelectrospray is a good marriage of sensitivity and specificity that has begun to receive attention (28)(29)(30)(31). Several groups have recently illustrated the usefulness of these capillary LC/ MS/MS on-line techniques with proteins and other biologically related analytes (29,32,33).…”

Section: Introductionmentioning

confidence: 99%

Determination of in Vitro- and in Vivo-Formed DNA Adducts of 2-Amino-3-methylimidazo[4,5-f]quinoline by Capillary Liquid Chromatography/Microelectrospray Mass Spectrometry

Gangl

Turesky

Vouros

1999

Chem. Res. Toxicol.

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Capillary liquid chromatography/microelectrospray mass spectrometry has been applied to the detection of deoxyribonucleoside adducts of the food-derived mutagen 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) from in vitro and in vivo sources. Constant neutral loss (CNL) and selective reaction monitoring (SRM) techniques with a triple-quadrupole mass spectrometer enabled sensitive and specific detection of IQ adducts in vitro and in animals. Detection of 1 adduct in 10(4) unmodified bases is achieved using CNL scanning detection, while the lower detection limits using SRM approach 1 adduct in 10(7) unmodified bases using 300 microg of DNA. The DNA adducts N-(deoxyguanosin-8-yl)-2-amino-3-methylimidazo[4, 5-f]quinoline (dG-C8-IQ) and 5-(deoxyguanosin-N(2)-yl)-2-amino-3-methylimidazo[4,5-f]quinoline (dG-N(2)-IQ) were detected in kidney tissues of chronically treated cynomolgus monkeys at levels and in proportions consistent with previously published (32)P-postlabeling data [Turesky, R. J., et al. (1996) Chem. Res. Toxicol. 9, 403-408]. Thus, capillary tandem LC/MS is a highly sensitive technique, which can be used to screen for DNA adducts in vivo.

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“…Later, DiCesare 36 reported the separation of four to five compounds in less than 1 min using very short conventional 100 mm = 4. 6 44,45 . These columns are similar to monolithic columns in that they do not contain end-frits and offer certain advantages over typical packed capillary columns.…”

Section: Introductionmentioning

confidence: 99%

Fast liquid chromatography/time-of-flight mass spectrometry using sol-gel bonded continuous-bed capillary columns

Collins

Tang

et al. 2000

J. Micro. Sep.

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Continuous‐bed capillary columns of 50 μm inside diameter, 360 μm outside diameter containing sol‐gel bonded large pore octadecylsilica particles (3 μm, 1500 Å) were examined for potential use in fast liquid chromatographic separations. Selected triazine herbicides and benzodiazepines were separated and detected using time‐of‐flight mass spectrometry. Interfacing and ionization were achieved through a home‐built liquid sheath electrospray ionization interface. Demonstration of optimum column performance was achieved through baseline separation of triazine herbicides using a column 25 cm in length. A short column, cut to the minimum physical distance between the column injector and the interface plate of the mass spectrometer (9.3 cm), was used to achieve separation of eight triazine herbicides and seven benzodiazepines in 40 and 35 s, respectively. A maximum syringe pump pressure (43 MPa) was applied to the shortened column, and conditions were optimized to obtain the best separation in the shortest amount of time for both triazine herbicides and benzodiazepines. The advantages of using time‐of‐flight mass spectrometry as a detector for fast separations are discussed. © 2000 John Wiley & Sons, Inc. J Micro Sep 12: 442–449, 2000

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Interfacing microbore and capillary liquid chromatography to continuous-flow fast atom bombardment mass spectrometry for the analysis of glycopeptides

Cited by 33 publications

References 14 publications

Microcolumn liquid chromatography: instrumentation, detection and applications

Microcolumn liquid chromatography: instrumentation, detection and applications

Determination of in Vitro- and in Vivo-Formed DNA Adducts of 2-Amino-3-methylimidazo[4,5-f]quinoline by Capillary Liquid Chromatography/Microelectrospray Mass Spectrometry

Fast liquid chromatography/time-of-flight mass spectrometry using sol-gel bonded continuous-bed capillary columns

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