Interference of Staphylococcus Aureus in the Detection of Chlamydia Trachomatis by Monoclonal Antibodies

Krech, T; Gerhard-Fsadni, D; Hofmann, Natalie; Miller, Stephan

doi:10.1016/s0140-6736(85)92467-5

Cited by 38 publications

(9 citation statements)

References 7 publications

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“…As a preliminary investigation of possible cross-reactivity of the commercial monoclonal antibody with oral microbes, the reagents were tested with microbes common to periodontitis and microbes reported in the literature to possibly cross-react with the DFA products ( Figure 1) [15,41,57,64]. The microbes were obtained from laboratories at the University of Michigan Schools of Dentistry and Public Health, and the Pathology Laboratory of the University of Michigan Hospital.…”

Section: Methodsmentioning

confidence: 99%

Oral Chlamydia trachomatis in patients with established periodontitis

Reed

Lopatin

Foxman

et al. 2000

Clinical Oral Investigations

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Periodontitis is considered a consequence of a pathogenic microbial infection at the periodontal site and host susceptibility factors. Periodontal research supports the association of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, and Bacteroides forsythus, and periodontitis; however causality has not been demonstrated. In pursuit of the etiology of periodontitis, we hypothesized that the intracellular bacteria, Chlamydia trachomatis, may play a role. As a first step, a cross-sectional study of dental school clinic patients with established periodontitis were assessed for the presence of C. trachomatis in the oral cavity, and in particular from the lining epithelium of periodontal sites. C. trachomatis was detected using a direct fluorescent monoclonal antibody (DFA) in oral specimens from 7% (6/87) of the patients. Four patients tested positive in specimens from the lining epithelium of diseased periodontal sites, one patient tested positive in healthy periodontal sites, and one patient tested positive in the general mucosal specimen. In conclusion, this study provides preliminary evidence of C. trachomatis in the periodontal sites. Planned studies include the use of a more precise periodontal epithelial cell collection device, the newer nucleic acid amplification techniques to detect C. trachomatis, and additional populations to determine the association of C. trachomatis and periodontitis.

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Section: Methodsmentioning

confidence: 99%

Oral Chlamydia trachomatis in patients with established periodontitis

Reed

Lopatin

Foxman

et al. 2000

Clinical Oral Investigations

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“…Disadvantages include the need of a fluorescent microscope and a trained microscopist to differentiate artifact from positive fluorescence. The monoclonal antibody reagent binds nonspecifically to staphylococcal Protein A making it possible to misinterpret some strains of Staphylococcus aureus as Chlamydia [9].…”

Section: Immunofluorescencementioning

confidence: 99%

Monoclonal antibodies in the laboratory diagnosis of trachoma

1988

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Various techniques which use monoclonal antibodies to detect Chlamydia trachomatis in clinical specimens are reviewed. An investigation comparing the efficacy of immunofluorescent staining with Giemsa staining in detecting Chlamydia in conjunctival scrapings from cases of active trachoma is presented. Sixty-two eyes of schoolboys with moderate to severe trachoma were studied. Giemsa staining detected chlamydial inclusion bodies in 34 percent of the specimens. Free elementary bodies were detected by fluorescent monoclonal antibody in 21 percent. Eleven percent were positive by both Giemsa and immunofluorescence and 55 percent were positive by either Giemsa and/or immunofluorescence. The addition of fluorescent monoclonal antibody assay to routine Giemsa staining resulted in an increase in the yield of positive specimens by 29 percent.

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“…However, in the DIF test, nonspecific binding of Fc receptor-bearing protein to labelled antibody may render some staphylococcal species fluorescent [39]. Furthermore, the presence of fluorescing artefactual bodies [40], which are seen most commonly in rectal specimens, may also give rise to false-positive results, particularly in inexperienced hands.…”

Section: Choice Of Methodsmentioning

confidence: 99%

The role of the microbiology laboratory in the investigation of child sexual abuse

Dyson

Hosein²

1996

Journal of Medical Microbiology

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Detection of a sexually transmitted pathogen in a child is suggestive of sexual abuse. Consequently, there are very strong clinical, social and legal reasons for diagnosing sexually transmitted disease in children correctly. Carefully considered protocols should be established for all stages of the microbiological investigation. All procedures, from specimen collection to report generation, should be undertaken to the highest possible standard with appropriate documentation. For the more commonly identified sexually transmitted pathogens in the paediatric population, the gold standard of diagnosis in the microbiology laboratory remains culture in vitro because this method offers maximum specificity. Whenever possible, culture must be followed by appropriate confirmatory tests. This highly exacting approach has significant resource and organisational implications and some tests may have to be centralised. The effective provision of an appropriate service for these cases is dependent on the local laboratory collaborating closely with hospital paediatricians and other microbiology laboratories.

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Interference of Staphylococcus Aureus in the Detection of Chlamydia Trachomatis by Monoclonal Antibodies

Cited by 38 publications

References 7 publications

Oral Chlamydia trachomatis in patients with established periodontitis

Oral Chlamydia trachomatis in patients with established periodontitis

Monoclonal antibodies in the laboratory diagnosis of trachoma

The role of the microbiology laboratory in the investigation of child sexual abuse

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