Interference of Xenoestrogen o,p′-DDT on the Action of Endogenous Estrogens at Environmentally Realistic Concentrations

Zhong, Lingying; Xiao, Xiang; Lü, Wei; Zhou, Pei; Wang, Lumei

doi:10.1007/s00128-013-0976-9

Cited by 6 publications

(5 citation statements)

References 16 publications

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“…This means the combinations with endogenous estrogens and environmental estrogens occur in additive way and contribute to the total estrogenicity present in human plasma. With a statistical method, mixtures effects of E 2 and o,p'-DDT were examined using pS2 and PR gene expressions in MCF-7 cells [34]. The results suggested that the xenoestrogen (o,p'-DDT) could act in an additive way with the E 2 , even at levels close to environmentally realistic concentrations [34].…”

Section: Research Of the Mixturesmentioning

confidence: 99%

“…With a statistical method, mixtures effects of E 2 and o,p'-DDT were examined using pS2 and PR gene expressions in MCF-7 cells [34]. The results suggested that the xenoestrogen (o,p'-DDT) could act in an additive way with the E 2 , even at levels close to environmentally realistic concentrations [34]. Nevertheless, considering the differences between in vitro and in vivo situation, the concentration in the environment or encountered by humans can not be fully compared with the medium concentration.…”

Section: Research Of the Mixturesmentioning

confidence: 99%

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Application of the MCF-7 Breast Cancer Cells in Endocrine-Disrupting Area

Zhong

Xiao

et al. 2014

AMR

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MCF-7 breast cancer cell line was first developed by Dr. Herbert Soule from a pleural effusion taken from a patient with metastatic breast cancer. This estrogen-responsive and estrogen receptor containing cell line can also express androgen, progesterone, glucocorticoid and retinoid receptors. It has been extensively used in identifying environmental estrogens and exploring the toxicity mechanisms, as well as the pathologic study and the disease treatment. This mini review article will focus on the development and application of MCF-7 cells in endocrine-disrupting area, especially in study of environmental estrogens.

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Section: Research Of the Mixturesmentioning

confidence: 99%

Section: Research Of the Mixturesmentioning

confidence: 99%

Application of the MCF-7 Breast Cancer Cells in Endocrine-Disrupting Area

Zhong

Xiao

et al. 2014

AMR

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“…Knowing that estrogen mediates pro-reproductive effects through actions in several organs of the female reproductive system (ovaries, fallopian tubes, uterus and placenta formation) and biochemical processes (Rosselli et al 2000;Deroo & Korach 2006;Zoloaga et al 2013), experimental studies of reproduction regarding the adverse effects caused by frequent exposure to xenoestrogen substances have been performed, reaffirming the importance and constant level of exposure at which the current world population is exposed to (Bromer et al 2010;Zhong et al 2013). Thus, the present study tested the hypothesis that melatonin can block or reduce the estrogenic effects of cimetidine in uterine stroma, interfering with ERα-receptor expression, content of collagen fibers and hormone levels (estrogen, progesterone and prolactin) in adult rats.…”

Section: Introductionmentioning

confidence: 97%

Associative administration of cimetidine and exogenous melatonin on the endometrial receptors of estrogen, collagen and hormone levels in adult rats

Silva¹,

Elias

Silva³

et al. 2020

PECEN

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Cloridrato de cimetidina, um bloqueador de receptores H2 das células parietais gástricas, que age reduzindo a secreção de ácido no estômago e tem sido estudado como substância xenoestrogênica. O uso crônico da cimetidina produz distúrbios homonais e toxidade no aparelho reprodutor masculino, além de reduzir o estradiol 2-hidroxilado e aumentar níveis séricos de estradiol e prolactina em mulheres, levando a hiperprolactinemia, que pode ser fator de risco para o câncer. A melatonina, neurohormônio sintetizado pela glândula pineal tem importante papel na função reprodutiva, regulando a produção de estrógeno, progesterrona e prolactina. O estudo testou a hipótese de que a melatonina pode bloquear ou reduzir os efeitos estrogênicos da cimetidina no estroma uterino, interferindo nos receptores de estrógeno, no teor de fibras colágenas e nos níveis hormonais em ratas adultas. Quarenta e cinco (45) ratas albinas divididas em três grupos: I – tratadas com placebo (controle); II – tratado com cimetidina (50 mg/kg) e III – tratado com cimetidina (50 mg/kg) associada à melatonina (200 μg/100 g). Os experimentos foram conduzidos por 7, 14 e 19 dias. Nos grupos tratados apenas com cimetidina, observou-se marcações mais intensas dos receptores REα, maior distribuição das fibras colágenas no endométrio, elevação dos níveis séricos de estrogênio, prolactina e redução da progesterona, nos animais tratados por 19 dias. Na associação cimetidina e melatonina, acredita-se que a melatonina bloqueou esses efeitos. A melatonina tem atividade citoprotetora para efeitos crônicos da cimetidina no estroma endometrial, por reduzir ou prevenir o aumento da síntese de fibras de colágeno pelos fibroblastos regulando a atividade do estrogênio sérico, bem como a expressão de seus receptores endometriais, além de manter os níveis normais de progesterona e prolactina.Palavras chave: Melatonina, receptor de estrógeno, xenoestrógeno, morfometria, níveis hormonais.

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“…From in vivo studies, o,p'-DDT has been found to be estrogenic, as measured by uterotrophic responses in mammals and vitellogenins production in fish [1,6]. O,p'-DDT has been identified as a relatively potent estrogen agonist in in vitro tests with estrogen-responsive tumor cells and in the yeast estrogen screening (YES) assay [2,7].…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Aptamers Against O,p’-DDT

Zhang

et al. 2020

IJMS

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The compound 1,1,1-trichloro-2-(p-chlorophenyl)-2-(o-chlorophenyl) ethane (o,p’-DDT) has been identified as one of the endocrine-disrupting chemicals causing adverse effects on wildlife and even humans through bioaccumulation. Its detection has become increasingly important. We have obtained candidate aptamers binding to o,p’-DDT by a systematic evolution of ligands by exponential enrichment (SELEX) protocol. Five out of seventeen candidate sequences were selected for preliminary characterization by SYBR Green I assay. One sequence with highest fluorescence response with o,p’-DDT, designated DDT_13, was chosen for further characterization. Its dissociation constant (Kd) was determined to be 412.3 ± 124.6 nM. DDT_13 exhibited low cross-binding activities on other tested small molecules. The good bioactivities of DDT_13 were demonstrated for the analysis of spiked lake water and tap water samples. This study provides a novel o,p’-DDT-specific probe for its future applications.

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Interference of Xenoestrogen o,p′-DDT on the Action of Endogenous Estrogens at Environmentally Realistic Concentrations

Cited by 6 publications

References 16 publications

Application of the MCF-7 Breast Cancer Cells in Endocrine-Disrupting Area

Application of the MCF-7 Breast Cancer Cells in Endocrine-Disrupting Area

Associative administration of cimetidine and exogenous melatonin on the endometrial receptors of estrogen, collagen and hormone levels in adult rats

Preparation and Characterization of Aptamers Against O,p’-DDT

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