Interferometric spatial frequency modulation imaging

Worts, Nathan; Field, Jeffrey J.; Bartels, Randy A.; Jones, J. A. A.; Broderick, Jeff; Squier, Jeff

doi:10.1364/ol.43.005351

Cited by 9 publications

(6 citation statements)

References 21 publications

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“…LSI has the potential to gather improved subsurface microvascular information due to the greater depth of penetration of these wavelengths compared to the visible range. This may spur the search for technological solutions to make LSI more of a volumetric imaging technique with depth‐resolved tomographic (or at least depth‐weighted) capabilities; in this context, it may be necessary to consider spatial frequency‐modulated imaging approaches [131]. Furthermore, the development of revolutionary camera technologies such as quantitative CMOS and dynamic vision sensors [132] will enable orders of magnitude increase in frame rates, boosting spatiotemporal resolution and allowing for enhanced visualization of the smallest microvessels including capillaries.…”

Section: Discussion and Outlookmentioning

confidence: 99%

Advances in laser speckle imaging: From qualitative to quantitative hemodynamic assessment

Qureshi,

Allam,

et al. 2023

Journal of Biophotonics

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Laser speckle imaging (LSI) techniques have emerged as a promising method for visualizing functional blood vessels and tissue perfusion by analyzing the speckle patterns generated by coherent light interacting with living biological tissue. These patterns carry important biophysical tissue information including blood flow dynamics. The non‐invasive, label‐free, and wide‐field attributes along with relatively simple instrumental schematics make it an appealing imaging modality in pre‐clinical and clinical applications. The review outlines the fundamentals of speckle physics and the three categories of LSI techniques based on their degree of quantification: qualitative semi‐quantitative and quantitative. Qualitative LSI produces microvascular maps by capturing speckle contrast variations between blood vessels containing moving red blood cells and the surrounding static tissue. Semi‐quantitative techniques provide a more accurate analysis of blood flow dynamics by accounting for the effect of static scattering on spatiotemporal parameters. Quantitative LSI such as optical speckle image velocimetry (OSIV) provides quantitative flow velocity measurements which is inspired by the particle image velocimetry in fluid mechanics. Additionally, discussions regarding the prospects of future innovations in LSI techniques for optimizing the vascular flow quantification with associated clinical outlook are presented.This article is protected by copyright. All rights reserved.

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Section: Discussion and Outlookmentioning

confidence: 99%

Advances in laser speckle imaging: From qualitative to quantitative hemodynamic assessment

Qureshi,

Allam,

et al. 2023

Journal of Biophotonics

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“…Since SPIFI was first introduced as an imaging technique, 22 it has been demonstrated and extended to multiple imaging modalities including fluorescent imaging, 22 , 23 multiphoton excitation fluorescence imaging, 24 – 26 second harmonic generation imaging, 26 , 27 phase imaging, 28 , 29 and localization microscopy 30 . Extending SPIFI to provide simultaneous SIM benefits in two dimensions has also been explored using 2D modulation schemes, 31 , 32 random-access imaging, 33 parallel line image acquisition, 34 single pixel tomographic imaging, 35 – 38 multi-cursor 2D imaging, 32 random access multiphoton imaging, 33 and coherent anti-Stokes Raman spectroscopy 27 …”

Section: Introductionmentioning

confidence: 99%

Cascaded domain multiphoton spatial frequency modulation imaging

Scarbrough,

Thomas,

Field

et al. 2023

J. Biomed. Opt.

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.SignificanceMultiphoton microscopy is a powerful imaging tool for biomedical applications. A variety of techniques and respective benefits exist for multiphoton microscopy, but an enhanced resolution is especially desired. Additionally multiphoton microscopy requires ultrafast pulses for excitation, so optimization of the pulse duration at the sample is critical for strong signals.AimWe aim to perform enhanced resolution imaging that is robust to scattering using a structured illumination technique while also providing a rapid and easily repeatable means to optimize group delay dispersion (GDD) compensation through to the sample.ApproachSpatial frequency modulation imaging (SPIFI) is used in two domains: the spatial domain (SD) and the wavelength domain (WD). The WD-SPIFI system is an in-line tool enabling GDD optimization that considers all material through to the sample. The SD-SPIFI system follows and enables enhanced resolution imaging.ResultsThe WD-SPIFI dispersion optimization performance is confirmed with independent pulse characterization, enabling rapid optimization of pulses for imaging with the SD-SPIFI system. The SD-SPIFI system demonstrates enhanced resolution imaging without the use of photon counting enabled by signal to noise improvements due to the WD-SPIFI system.ConclusionsImplementing SPIFI in-line in two domains enables full-path dispersion compensation optimization through to the sample for enhanced resolution multiphoton microscopy.

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“…In DHM, many different interferometric configurations have been implemented to work in reflection, such as modified Mach-Zehnder [6][7][8][9], Michelson [10][11][12][13], Linnik [14], Twyman-Green [15], Sagnac [16], and common-path interferometers [17][18][19][20][21][22][23], among others. Perhaps the modified Mach-Zehnder and Michelson interferometers are the most common used layouts.…”

Section: Introductionmentioning

confidence: 99%

“…For instance, such a beam can be created: (1) by the reflection of the light in a reference mirror inside a Mirau interferometric objective [18]; (2) by a spatial filtering at the Fourier plane in diffraction phase microscopy [17,19]; by using the clear regions near to sparse samples in lateral shearing interferometry [24][25][26][27], (3) by spatially-multiplexing the field of view (FOV) [23], or (4) by using a modulator mask in single pixel phase imaging [21,22].…”

Section: Introductionmentioning

confidence: 99%

Single-Element Reflective Digital Holographic Microscopy

2021

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Digital holographic microscopy (DHM) is a well-known microscopy technique using an interferometric architecture for quantitative phase imaging (QPI) and it has been already implemented utilizing a large number of interferometers. Among them, single-element interferometers are of particular interest due to its simplicity, stability, and low cost. Here, we present an extremely simple common-path interferometric layout based on the use of a single one-dimensional diffraction grating for both illuminating the sample in reflection and generating the digital holograms. The technique, named single-element reflective digital holographic microscopy (SER-DHM), enables QPI and topography analysis of reflective/opaque objects using a single-shot operation principle. SER-DHM is experimentally validated involving different reflective samples.

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Interferometric spatial frequency modulation imaging

Cited by 9 publications

References 21 publications

Advances in laser speckle imaging: From qualitative to quantitative hemodynamic assessment

Advances in laser speckle imaging: From qualitative to quantitative hemodynamic assessment

Cascaded domain multiphoton spatial frequency modulation imaging

Single-Element Reflective Digital Holographic Microscopy

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