Interferon Gamma Administration Increases Monocyte HLA-DR Antigen Expression but Not Endogenous Interferon Production

Livingston, David H.; Loder, Patricia; Kramer, Susan M.; Gibson, Ursula E. M.; Polk, Hiram C.

doi:10.1001/archsurg.1994.01420260068009

Cited by 32 publications

(20 citation statements)

References 24 publications

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“…Administration of IFN-␥ has been investigated for clinical efficacy in trauma and burn patients (27,41,42,56). Our results, coupled with the findings in previous reports, seem to indicate that systemic administration of IFN-␥ alone for prophylaxis, or treatment, of nosocomial infections might not be an effective clinical strategy.…”

Section: Discussionmentioning

confidence: 48%

Gamma Interferon Does Not Enhance Clearance ofPseudomonas aeruginosabut Does Amplify a Proinflammatory Response in a Murine Model of Postseptic Immunosuppression

2004

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Patients that have suffered a major injury may sustain a period of immunocompromise and altered Th1/Th2 cytokine balance that can predispose them to opportunistic infections. Pseudomonas aeruginosa is frequently a causative organism for nosocomial infections in critically ill patients and is associated with high mortality. We previously mimicked this clinical scenario by challenging mice with P. aeruginosa 5 days after a cecal ligation and puncture (CLP) procedure. Mice that were subjected to CLP had reduced ability to clear bacteria, significantly lower gamma interferon (IFN-␥) concentrations in plasma, and significantly elevated levels of interleukin 10 (IL-10) in plasma in response to the Pseudomonas challenge compared to uninjured control mice.

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Section: Discussionmentioning

confidence: 48%

Gamma Interferon Does Not Enhance Clearance ofPseudomonas aeruginosabut Does Amplify a Proinflammatory Response in a Murine Model of Postseptic Immunosuppression

2004

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“…Several investigators have demonstrated that use of IFN-g for the treatment of patients with sepsis leads to rescue from severe sepsis and to enhanced HLA-DR expression [22,26,27]. In cell culture experiments, we have already shown in vitro that the expression of HLA-DR and FcgRI/CD64, as well as the ability of monocytes to phagocytose Ig-opsonized E. coli, is augmented by IL-6 and TNF-a [36,37].…”

Section: Discussionmentioning

confidence: 90%

“…APACHE, acute physiology and chronic health evaluation; SAPS, simplified acute physiology score. ment of patients with sepsis restored reduced HLA-DR expression on monocytes and rescued patients from severe sepsis [22,26,27].…”

mentioning

confidence: 92%

Relationship between Interleukin‐6 Plasma Concentration in Patients with Sepsis, Monocyte Phenotype, Monocyte Phagocytic Properties, and Cytokine Production

Spittler

Razenberger

Küpper³

et al. 2000

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Monocyte phenotype, their phagocytic capacity as well as the cytokine production from 10 patients with sepsis with low interleukin-6 (IL-6) serum concentrations (<1000 pg/mL) and 8 patients with sepsis with high IL-6 (> or = 1000 pg/mL) plasma concentrations were investigated within 24 hours of fulfilling the criteria for sepsis. Monocytes from patients with high IL-6 levels had higher levels of human leukocyte antigen (HLA)-DR, HLA-ABC, CD64, and CD71, and the production of tumor necrosis factor-alpha (TNF-alpha) and IL-8, as well as the capacity of monocytes to phagocytose, was significantly elevated. Of 8 patients with high levels of plasma IL-6, 4 patients died. In contrast, all 10 patients with low plasma IL-6 concentrations survived until day 28. Patients who died had constant high IL-6 concentrations during the first 3 days, whereas IL-6 levels in patients who survived decreased by 88%. Our data indicate that IL-6 levels are a better prognostic parameter in the early phase of sepsis than the monocyte HLA-DR expression.

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“…Studies have shown that the degree of monocyte class II MHC expression is related to outcome [6,12], the incidence of infection [5,11] and survival [6,17]. Although interferon-c has been shown to up-regulate the expression of monocyte class II MHC expression [18], the postoperative decrease in class II MHC expression is resistant to immunomodulation with interferon-c [19]. Monocyte class II MHC expression may be an appropriate means of monitoring overall immune function postoperatively.…”

Section: Discussionmentioning

confidence: 99%

Decreased monocyte class II MHC expression following major abdominal surgery in children is related to operative stress

et al. 2006

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Monocyte class II major histocompatibility complex (MHC) expression is necessary for antigen presentation and stimulation of T-cells. The aim of this study was to correlate monocyte class II MHC response to operative stress in children and the possible influence of cytokines in the postoperative period. We studied 21 children undergoing elective abdominal surgery. Operative stress score (OSS) was calculated. Monocyte class II MHC expression was measured preoperatively, immediately after surgery, 24 and 48 h postoperatively, using flow cytometry. Class II MHC is expressed as mean fluorescence intensity (MFI) of monocytes expressing MHC (mean +/- SD). Cytokine levels (interleukins 1ra, 6, and 10, and tumor necrosis factor-alpha) were also measured. Data between time points were compared using repeated measures ANOVA. There was an immediate postoperative decrease in class II MHC expression, with lowest levels 24 h postoperatively (preoperative 50 +/- 23.6, 24 h 18.2 +/- 9.4, P < 0.0001 vs. preoperative). At 48 h there was partial recovery in class II MHC, but levels were still significantly lower than preoperative (23.9 +/- 11.1, P < 0.001). The degree of monocyte depression was related to the magnitude of operative stress. Patients who had OSS <10 displayed some recovery in expression at 48 h 25.5 +/- 11.1), whereas in patients with OSS > or = 10 (severe surgical stress), expression further decreased at 48 h (MFI 14.0 +/- 0.1). There was an elevation of interleukin-1ra in the immediate postoperative period in both groups. There was no elevation in the other cytokines. Abdominal surgery in children decreases monocyte MHC expression. Class II MHC depression was related to magnitude of surgical trauma, implying that more severe immuneparesis follows surgery of greater magnitude. This may predispose to postoperative infection.

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Interferon Gamma Administration Increases Monocyte HLA-DR Antigen Expression but Not Endogenous Interferon Production

Cited by 32 publications

References 24 publications

Gamma Interferon Does Not Enhance Clearance ofPseudomonas aeruginosabut Does Amplify a Proinflammatory Response in a Murine Model of Postseptic Immunosuppression

Gamma Interferon Does Not Enhance Clearance ofPseudomonas aeruginosabut Does Amplify a Proinflammatory Response in a Murine Model of Postseptic Immunosuppression

Relationship between Interleukin‐6 Plasma Concentration in Patients with Sepsis, Monocyte Phenotype, Monocyte Phagocytic Properties, and Cytokine Production

Decreased monocyte class II MHC expression following major abdominal surgery in children is related to operative stress

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