Interferon Gamma Gene Polymorphism (+874 T > A) and Chronic Hepatitis B in the Population of Gorgan, North-Eastern Iran

Abstract

BackgroundBased on differences in individual immune responses to the hepatitis B virus (HBV), between 5% and 10% of patients become persistently infected with the virus, which leads to the determination of chronic HBV. Cytokines such as interferon gamma (IFN-γ) are secretory proteins that play important roles in both innate and adaptive immune responses. Functional studies have demonstrated that the IFN + 874A/T gene polymorphism can increase or decrease the overall expression of IFN-gamma (γ) and ultimately determine the outcome of the infection.ObjectivesThis study was performed to investigate the relationship between the IFN-γ + 874 gene polymorphism and susceptibility to chronic HBV infection.MethodsPolymorphism detection analysis was performed on 598 subjects from North-Eastern Iran. The IFN-γ gene polymorphism (+ 874A/T) was genotyped through a specific sequence primer polymerase chain reaction (SSP-PCR).ResultsThe frequencies of the AA, AT, and TT genotypes were 31%, 51%, and 18% in the chronic HBV patient group, and 40%, 45%, and 15% in the healthy control group, respectively. However, a lack of association of the + 874 polymorphism in the IFN-γ gene of those with chronic HBV infection was found. Evaluation of HBV association with this polymorphism was significant under the dominant genetic model (P = 0.04).ConclusionsUltimately, no association could be characterized between the polymorphism in IFN-γ + 874A/T and susceptibility to chronic HBV infection in this segment of the Iranian population (P > 0.05).

Single Nucleotide Polymorphism of the Interferon-γ Gene (IFN-γ +874 T/A) and the Prognosis of Hepatitis B Infection

“…Also in agreement with our results, in a rural area of Northern China IFN-γ+ 874 AA genotype showed to be associated with an increased risk of chronic HBV infection [30]. On the other hand, a study from Iran in 2016 showed no association between this polymorphism and HBV clearance in a population from Golestan Province [31]. The difference in results might be attributed to the ethnic background of the studied populations, sample size, and the genotyping method.…”

Association of Interleukin 13/+110 Gene Polymorphism with Hepatitis B Virus Infection in Golestan Province, Northern Iran

“…Polymorphisms in genes encoding cytokines may be the primary cause of clinical differences between patients; hence they can be used for the identification of individuals who are at high risk of developing hepatocellular carcinoma and chronic hepatitis (3,36). So far, we have evaluated the association of several genes with hepatitis and cancer (27,(47)(48)(49)(50)(51).…”

Association between the interferon-γ +874T/A polymorphism and susceptibility to hepatitis B virus infection: a meta-analysis

“…Thirteen articles met the inclusion criteria. [13][14][15][16][18][19][20][21][22][23][24][25][26] The literature selection process is shown in Figure 1. The characteristics of the included studies, genotype distribution frequency, and literature quality scores are shown in Table 1.…”

“…11 HBV infection levels were found to decrease with increasing IFN-c expression in chimpanzees, 12 so the susceptibility to HBV is thought to be associated with IFN-c gene SNPs. Yu et al 13 and Korachi et al 14 found that the T allele at IFN-c þ874 was a protective factor against HBV infection, Arababadi et al 15 reported that the T allele was not associated with HBV susceptibility, while Ghasemian et al 16 showed that it was a risk factor for HBV infection. Because the conclusions of these previous studies are inconsistent, we herein used a meta-analysis to investigate whether the IFN-c þ874 SNP is associated with susceptibility to HBV infection.…”

“… 15 reported that the T allele was not associated with HBV susceptibility, while Ghasemian et al. 16 showed that it was a risk factor for HBV infection. Because the conclusions of these previous studies are inconsistent, we herein used a meta-analysis to investigate whether the IFN-γ +874 SNP is associated with susceptibility to HBV infection.…”