Interferon receptors

Branca, Andrew A.

doi:10.1007/bf02623541

Cited by 28 publications

(7 citation statements)

References 122 publications

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“…IFNa comprises at least 13 subtypes (a1, a2, a4, etc.) of structurally similar glycoproteins that share a common receptor on eukaryotic cells, namely IFN-a/b receptor (IFNAR) (Branca, 1988). Generation of cellular responses to IFN-a is mediated by the coordination and cooperation of multiple distinct signalling cascades, including the Janus-activated kinase (JAK)-signal transducer and activator of transcription (STAT) pathway (Platanias, 2005).…”

Section: Introductionmentioning

confidence: 99%

Altered chemotactic response of myeloid and plasmacytoid dendritic cells from patients with chronic hepatitis C: role of alpha interferon

Cicinnati

Kang

Sotiropoulos

et al. 2008

Journal of General Virology

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Dendritic cell (DC) frequencies in the blood of patients with chronic hepatitis C virus (HCV) infection have been shown to be reduced significantly compared with those in healthy individuals. There is a further reduction of circulating myeloid DCs (MDCs) and plasmacytoid DCs (PDCs) in HCV patients receiving alpha interferon (IFN-a)-based antiviral therapy. Altered homing behaviour of DCs may be a possible mechanism for their 'loss' in peripheral blood in these clinical conditions. Systemic chemokine levels were measured by ELISA. Phenotypes and migratory properties of MDCs and PDCs from HCV patients were analysed by flow cytometry and chemotaxis assay. Compared with healthy controls, HCV patients had increased serum levels of inflammatory and constitutively expressed chemokines. Spontaneously generated MDCs from HCV patients were less mature, and both MDCs and PDCs showed intrinsic activation of receptors for inflammatory chemokines, thus suggesting an increased propensity to migrate towards inflammatory sites. IFN-a treatment in vitro induced MDC maturation and skewed the migratory response of both MDCs and PDCs towards chemokines expressed constitutively in secondary lymphoid organs. In conclusion, our results hint at altered homing behaviour of DCs during chronic HCV infection. IFN-a therapy may redirect DC migration from inflamed hepatic portal areas towards secondary lymphoid tissue.

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Section: Introductionmentioning

confidence: 99%

Altered chemotactic response of myeloid and plasmacytoid dendritic cells from patients with chronic hepatitis C: role of alpha interferon

Cicinnati

Kang

Sotiropoulos

et al. 2008

Journal of General Virology

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“…The first step of IFN action requires specific cell-surface receptors. IFN-a and IFN-ß share a common receptor which is distinct from the IFN-receptor (Pestka et al 1987;Branca, 1988).…”

Section: Introductionmentioning

confidence: 99%

Modulation of differentiation of rat granulosa cells in vitro by interferon-γ

Xiao¹,

Findlay²

1992

Journal of Endocrinology

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The effects of recombinant rat interferon-gamma (rRaIFN-gamma) and rat IFN (RaIFN, a mixture of IFN-gamma and -alpha) on basal and FSH-induced ovarian granulosa cell function were studied. Granulosa cells were harvested from diethylstilboestrol-treated immature rats and cultured (2 x 10(5) viable cells/well per 0.5 ml) in serum-free medium with or without treatment for 48 h. In the presence of FSH (20 ng/ml), rRaIFN-gamma (10-1000 U/ml) significantly inhibited FSH-stimulated aromatase activity (76.4 +/- 2.3% maximum inhibition compared with FSH treatment alone), inhibin (40.4 +/- 3.7%), progesterone (47.7 +/- 8.6%) and 20 alpha-hydroxypregn-4-en-3-one (20 alpha-OHP) (51.8 +/- 1.7%) production in a dose-dependent manner. Furthermore, rRaIFN-gamma inhibited FSH- and forskolin (FSK; 30 mumol/l)-induced extracellular cAMP accumulation (46.0 +/- 6.6% and 29.1 +/- 7.3% respectively). The inhibitory effect of rRaIFN-gamma on FSK-induced cAMP was accompanied by decreased FSK-induced aromatase activity, inhibin, progesterone and 20 alpha-OHP production. rRaIFN-gamma had no detectable effect on aromatase activity, progesterone production and 20 alpha-OHP production in the absence of FSH, but significantly stimulated basal inhibin production by 1.5-fold. rRaIFN-gamma alone also caused a small but significant increase in basal levels of cAMP. The time-course studies showed that FSH-induced aromatase activity and inhibin production were consistently suppressed by rRaIFN-gamma, FSH-induced progesterone and 20 alpha-OHP were inhibited at 1 and 2 days and then stimulated on days 3, 4 and 5 relative to FSH alone.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…IFNs exert antiviral, antimicrobial, antitumor, and immunomodulatory effects. These effects are mediated by the binding of IFN to specific receptors on the cell surface, signaling transduction through the nucleus and activating specific genes (1)(2)(3). IFNs are assigned to two classes, type I and type II IFN, according to their interaction with specific receptors (4)(5)(6)(7).…”

Section: Introductionmentioning

confidence: 99%

Expression and biological activity of two recombinant polypeptides related to subunit 1 of the interferon-a receptor

Yoon

Hirata

Nguyen

et al. 2000

Braz J Med Biol Res

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Abnormal production of interferon alpha (IFN-=) has been found in certain autoimmune diseases and can be also observed after prolonged therapy with IFN-=. IFN-= can contribute to the pathogenesis of allograft rejection in bone marrow transplants. Therefore, the development of IFN-= inhibitors as a soluble receptor protein may be valuable for the therapeutic control of these diseases. We have expressed two polypeptides encoding amino acids 93-260 (P1) and 261-410 (P2) of the extracellular domain of subunit 1 of the interferon-= receptor (IFNAR 1-EC) in E. coli. The activities of the recombinant polypeptides and of their respective antibodies were evaluated using antiproliferative and antiviral assays. Expression of P1 and P2 polypeptides was achieved by transformation of cloned plasmid pRSET A into E. coli BL21(DE3)pLysS and by IPTG induction. P1 and P2 were purified by serial sonication steps and by gel filtration chromatography with 8 M urea and refolded by dialysis. Under reducing SDS-PAGE conditions, the molecular weight of P1 and P2 was 22 and 17 kDa, respectively. Polyclonal anti-P1 and anti-P2 antibodies were produced in mice. P1 and P2 and their respective polyclonal antibodies were able to block the antiproliferative activity of 6.25 nM IFN-=B on Daudi cells, but did not block IFN-=B activity at higher concentrations (>6.25 nM). On the other hand, the polypeptides and their respective antibodies did not inhibit the antiviral activity of IFN-=B on Hep 2/c cells challenged with encephalomyocarditis virus. Correspondence

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Interferon receptors

Cited by 28 publications

References 122 publications

Altered chemotactic response of myeloid and plasmacytoid dendritic cells from patients with chronic hepatitis C: role of alpha interferon

Altered chemotactic response of myeloid and plasmacytoid dendritic cells from patients with chronic hepatitis C: role of alpha interferon

Modulation of differentiation of rat granulosa cells in vitro by interferon-γ

Expression and biological activity of two recombinant polypeptides related to subunit 1 of the interferon-a receptor

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