Modulation of differentiation of rat granulosa cells in vitro by interferon-γ

Xiao, Shen‐Hua; Findlay, J. K.

doi:10.1677/joe.0.1330131

Cited by 18 publications

(12 citation statements)

References 18 publications

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“…However, IFN is present in follicular fluid [24], and lymphocytes that infiltrate the ovary could provide a source of IFN [25,26]. IFN has been shown to inhibit LH receptor induction [27] and gonadotropinstimulated steroidogenesis [27][28][29], to alter inhibin production by granulosa cells [29], and to inhibit progesterone secretion by bovine luteal cells [30]. Further studies are required to determine factors relevant to the modulation of the Fas pathway in ovarian cells in vivo.…”

Section: Discussionmentioning

confidence: 99%

Expression and Activity of the Fas Antigen in Bovine Ovarian Follicle Cells1

Vickers

Cowan

Harman

et al. 2000

Biology of Reproduction

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The Fas antigen is a cell surface receptor that triggers apoptosis when bound to Fas ligand (FasL). Studies were undertaken to determine whether the cow provides a suitable model to study the role of the Fas pathway in inducing apoptosis of ovarian cells during follicular atresia. Expression of Fas antigen mRNA and responsiveness to FasL-induced killing in vitro were measured. Effects of the cytokines tumor necrosis factor (TNF)-alpha and interferon-gamma (IFN) were studied because of previous demonstrations of their role in Fas-mediated apoptosis in other cell types. Fas antigen mRNA was detectable in cultured granulosa and theca cells, and expression was increased by treatment with IFN but not TNF. Granulosa and theca cells were resistant to FasL-induced killing unless pretreated with IFN. TNF had no effect on FasL-induced killing. Granulosa and theca cell cultures in which killing occurred in response to FasL stained positively for annexin V, an early marker for cells undergoing apoptosis. These results provide a basis for further studies using the bovine ovary to examine the role of the Fas antigen in follicular atresia.

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Section: Discussionmentioning

confidence: 99%

Expression and Activity of the Fas Antigen in Bovine Ovarian Follicle Cells1

Vickers

Cowan

Harman

et al. 2000

Biology of Reproduction

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“…EGF, TGF-oa, FSP, interferon-y, and high doses of LH negatively regulate inhibin production in the presence of FSH and other agents that increase cAMP levels [10]. Activin and interferon-y also stimulate basal inhibin production [11][12][13]. While there is evidence for intraovarian production of most of the regulators mentioned above, it remains to be established whether or not interferon-y is made by the ovarian follicle.…”

Section: Inhibinmentioning

confidence: 99%

An Update on the Roles of Inhibin, Activin, and Follistatin as Local Regulators of Folliculogenesis1

Findlay¹

1993

Biology of Reproduction

401

211

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The local actions of the inhibin-related peptides inhibin, activin, and follistatin in folliculogenesis, luteinization and atresia are reviewed. On the basis of in vitro data, it is concluded that (a) inhibin has a paracrine action positively regulating LH-induced androgen production by theca cells in addition to a peripheral role regulating FSH secretion; (b) activin has a negative paracrine action on LH-induced androgen production by theca cells; (c) activin, either alone or with FSH, has an autocrine action on granulosa cells, promoting differentiation during the preantral and early antral stages of folliculogenesis and preventing premature luteinization in the later stages of antral follicle development, leading overall to promotion and maintenance of the folliculogenic state of the follicle; and (d) follistatin modulates granulosa cell function in favor of luteinization or atresia, and its mechanism of action involves neutralizing the actions of activin by its binding properties and by a direct action of follistatin on progesterone metabolism by granulosa cells. A hypothesis for a role of activin in the acquisition of responsiveness of granulosa cells of preantral follicles to FSH is proposed.

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“…This appears to be the first demonstration of an ovarian effect of -interferon. The chemically and function¬ ally unrelated gamma-interferon has been shown to decrease progesterone and increase prostaglandin secretion by bovine luteal cells (Fairchild & Pate, 1991) and to inhibit differentiation in rat granulosa cells (Gorospe et al, 1988;Xiao & Findlay, 1992).…”

Section: Discussionmentioning

confidence: 99%

DIRECT STIMULATION OF BOVINE OVARIAN PROGESTERONE SECRETION BY LOW CONCENTRATIONS OF a-INTERFERON

Lück

Shale²,

Payne³

1992

Journal of Endocrinology

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The ruminant conceptus secretes proteins during early pregnancy which maintain the corpus luteum. These trophoblast proteins are related to the alpha II-interferons and prevent luteolysis indirectly by disrupting the secretion of endometrial prostaglandin. Although trophoblast interferons appear to be largely confined to the uterine lumen, it remains possible that they also act peripherally. This report describes in vitro studies which suggest that interferon may influence hormone secretion by the ovary directly. The study employed i) a well defined serum-free culture model in which bovine granulosa cells secrete the luteal hormones progesterone and oxytocin, and ii) serum-free and serum-supplemented cultures of cells from early CL. Dose-response experiments were performed using bovine recombinant alpha-interferon (brIFN). Progesterone and oxytocin secretions were measured over 4-5 days of culture and DNA content was also determined. Low concentrations of brIFN (10(-15) mol/l to 10(-11) mol/l) stimulated progesterone secretion by granulosa cells by up to three fold, without significantly affecting oxytocin concentrations or culture DNA content. Concentrations of 10(-10) mol/l to 10(-1) mol/l suppressed progesterone secretion in a log dose-related manner (r = 0.97) with evidence of toxicity (lower oxytocin concentrations and significantly reduced DNA compared with controls). Progesterone secretion by luteal cells in serum-free culture was stimulated in the presence of 10(-15) mol/l brIFN, whilst high concentrations again caused inhibition. The data show that ovarian cells can respond directly to low concentrations of interferon-like proteins.(ABSTRACT TRUNCATED AT 250 WORDS)

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Modulation of differentiation of rat granulosa cells in vitro by interferon-γ

Cited by 18 publications

References 18 publications

Expression and Activity of the Fas Antigen in Bovine Ovarian Follicle Cells1

Expression and Activity of the Fas Antigen in Bovine Ovarian Follicle Cells1

An Update on the Roles of Inhibin, Activin, and Follistatin as Local Regulators of Folliculogenesis1

DIRECT STIMULATION OF BOVINE OVARIAN PROGESTERONE SECRETION BY LOW CONCENTRATIONS OF a-INTERFERON

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