Interferon regulatory factors (IRFs) repress transcription of the chicken ovalbumin gene

Dougherty, Dawne C.; Park, Hyi Man; Sanders, Michel M.

doi:10.1016/j.gene.2009.03.016

Cited by 9 publications

(7 citation statements)

References 26 publications

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“…Previous research has largely focused on the function of individual IRFs in mammals [ 8 , 11 , 12 ]. However, the composition and the function of these IRF family members are less investigated in other classes of vertebrates [ 13 ]. In fish, a few members, such as IRF-1, IRF-2 and IRF-7 have been cloned in some aquacultured species [ 14 - 18 ], and recently a total of eleven IRF family members has been identified in zebrafish Dario rerio [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Global characterization of interferon regulatory factor (IRF) genes in vertebrates: Glimpse of the diversification in evolution

et al. 2010

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BackgroundInterferon regulatory factors (IRFs), which can be identified based on a unique helix-turn-helix DNA-binding domain (DBD) are a large family of transcription factors involved in host immune response, haemotopoietic differentiation and immunomodulation. Despite the identification of ten IRF family members in mammals, and some recent effort to identify these members in fish, relatively little is known in the composition of these members in other classes of vertebrates, and the evolution and probably the origin of the IRF family have not been investigated in vertebrates.ResultsGenome data mining has been performed to identify any possible IRF family members in human, mouse, dog, chicken, anole lizard, frog, and some teleost fish, mainly zebrafish and stickleback, and also in non-vertebrate deuterostomes including the hemichordate, cephalochordate, urochordate and echinoderm. In vertebrates, all ten IRF family members, i.e. IRF-1 to IRF-10 were identified, with two genes of IRF-4 and IRF-6 identified in fish and frog, respectively, except that in zebrafish exist three IRF-4 genes. Surprisingly, an additional member in the IRF family, IRF-11 was found in teleost fish. A range of two to ten IRF-like genes were detected in the non-vertebrate deuterostomes, and they had little similarity to those IRF family members in vertebrates as revealed in genomic structure and in phylogenetic analysis. However, the ten IRF family members, IRF-1 to IRF-10 showed certain degrees of conservation in terms of genomic structure and gene synteny. In particular, IRF-1, IRF-2, IRF-6, IRF-8 are quite conserved in their genomic structure in all vertebrates, and to a less degree, some IRF family members, such as IRF-5 and IRF-9 are comparable in the structure. Synteny analysis revealed that the gene loci for the ten IRF family members in vertebrates were also quite conservative, but in zebrafish conserved genes were distributed in a much longer distance in chromosomes. Furthermore, all ten different members are clustered in respectively different clades; but the IRF-11 was clustered with one in sea urchin.ConclusionsIn vertebrates, the ten well-characterized IRF family members shared a relatively high degree of similarity in genomic structure and syntenic gene arrangement, implying that they might have been evolved in a similar pattern and with similar selective pressure in different classes of vertebrates. Genome and/or gene duplication, and probably gene shuffling or gene loss might have occurred during the evolution of these IRF family members, but arrangement of chromosome or its segment might have taken place in zebrafish. However, the ten IRF family members in vertebrates and those IRF-like genes in non-vertebrate deuterostomes were quite different in those analyzed characters, as they might have undergone different patterns of evolution.

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Section: Introductionmentioning

confidence: 99%

Global characterization of interferon regulatory factor (IRF) genes in vertebrates: Glimpse of the diversification in evolution

et al. 2010

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“…The synthesis of several therapeutic proteins under the control of regulatory sequences from the chicken Ovalbumin gene has been reported (Byun et al, 2011;Cao et al, 2015;Herron et al, 2018;Kodama et al, 2012 Park et al, 2015;Zhu et al, 2005). Although the regulatory elements in theOvalbumin gene are well characterized out of their genomic context (Dougherty et al, 2009;Dougherty & Sanders, 2005;Haecker et al, 1995;Kato et al, 1992;Kaye et al, 1984Kaye et al, , 1986Monroe & Sanders, 2000; H. M. Park et al, 2000;Sanders & McKnight, 1988;Schimke et al, 1975;Schweers et al, 1990;Sensenbaugh & Sanders, 1999;Wang et al, 1989), it is not clear what regulatory sequences of the ovalbumin promoter are sufficient and efficient enough for inducing oviduct-specific expression of exogenous genes in the bioreactor chickens. In plasmid constructs, various lengths of chicken ovalbumin promoter fragments and, 5' and 3' flanking regions have been fused to the exogenous genes in order to induce gene expression.…”

Section: Discussionmentioning

confidence: 99%

“…The regulatory sequences of the ovalbumin promoter, which has been extensively used in the chicken production systems are well-characterized (Dougherty, Park, & Sanders, 2009;Dougherty & Sanders, 2005;Haecker, Muramatsu, Sensenbaugh, & Sanders, 1995;Kato et al, 1992;Kaye, Bellard, Dretzen, Bellard, & Chambon, 1984;Kaye et al, 1986;Monroe & Sanders, 2000;H. M. Park, Haecker, Hagen, & Sanders, 2000;Sanders & McKnight, 1988;Schimke, McKnight, Shapiro, Sullivan, & Palacios, 1975;Schweers, Frank, Weigel, & Sanders, 1990;Sensenbaugh & Sanders, 1999;Wang et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

CRISPR/CAS-mediated deletion of the upstream regulatory sequences enhances the estrogen-independent expression of chicken ovalbumin

Taemeh

Dehdilani

Goshayeshi

et al. 2020

Preprint

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Avian transgenesis has served as a suitable approach to generate bioreactors for the manufacturing of recombinant proteins. Production in chicken cells comes with significant advantages over other systems including providing the human-like glycosylation on target proteins. In this regard, the oviduct-specific ovalbumin promoter has been one of the ideal candidates to drive the expression of transgenes. Previous plasmid-based studies on the regulatory sequences of ovalbumin promoter have led researchers to exploit ovalbumin regulatory elements out of their native genomic context (ex situ) to direct transgene expression in the transgenic chicken bioreactors. Although the inherent limitations on the ex situ use of ovalbumin promoter have promoted the use of native ovalbumin promoter for the expression of a transgene, generation of transgenic chicken is relatively difficult, inefficient, and time-consuming. To overcome these obstacles, in this study we show that CRISPR-mediated deletion of some distal ovalbumin promoter sequences in a non-oviduct cell can lead to the significant expression of the ovalbumin gene, and also a knocked-in reporter, in an estrogen-independent manner. These findings overcome the limitation of cloned promoters, where the promoter regulatory sequences have to be taken out of their cis context and also their native spatial nuclear organization into a plasmid.

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“…Repression of ovalbumin gene in non-oviduct tissue and in estrogen-deprived oviduct depends on a strong repressor site located from −130 to −100 and designated CAR for COUP-TF adjacent repressor [18].…”

Section: Tissue Specificitymentioning

confidence: 99%

The Family Secrets of Avian Egg-Specific Ovalbumin and Its Related Proteins Y and X

Silva¹,

Beauclercq²,

Harichaux³

et al. 2015

Biology of Reproduction

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The ovalbumin gene family in Gallus gallus is composed of three homologous genes located within a 46 kb locus on chromosome 2: ovalbumin, ovalbumin-related protein Y (OVAY), and ovalbumin-related protein X (OVAX) genes. The expression of these genes in hen oviduct is under estrogen control, but their relative hormonal responsiveness and subsequent protein concentration in egg, is distinctive. Interestingly, all three proteins lack the classical signal peptide for secretion. Ovalbumin, OVAX, and OVAY belong to the serine protease inhibitor (serpin) family whose members share a common tertiary structure. Ovalbumin and OVAX are one of the few members of this family that do not express any protease inhibition activity whereas OVAY has been predicted to be inhibitory, by comparison with the consensus sequence for inhibitory serpins. In contrast to ovalbumin and OVAY, OVAX interacts with heparin, a negatively charged glycosaminoglycan, via a positively charged domain exposed at the surface of the molecule. Ovalbumin is the major egg white protein and might be a source of amino acids for the developing embryo. The physiological function of OVAY is not known, but recent data have revealed a possible role of this protein in early embryonic development. Considering the antibacterial activities of OVAX, this protein might play a role in egg defense. This review sheds light on the expression, biochemistry, and structural specificities of these three highly similar paralogs. It gives new clues in favor of diverging functions, which are likely to have arisen by duplication events from a common ancestral gene.

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Interferon regulatory factors (IRFs) repress transcription of the chicken ovalbumin gene

Cited by 9 publications

References 26 publications

Global characterization of interferon regulatory factor (IRF) genes in vertebrates: Glimpse of the diversification in evolution

Global characterization of interferon regulatory factor (IRF) genes in vertebrates: Glimpse of the diversification in evolution

CRISPR/CAS-mediated deletion of the upstream regulatory sequences enhances the estrogen-independent expression of chicken ovalbumin

The Family Secrets of Avian Egg-Specific Ovalbumin and Its Related Proteins Y and X

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