Intergenomic evolution and metabolic cross-talk between rumen and thermophilic autotrophic methanogenic archaea

Bharathi, M.; Chellapandi, P.

doi:10.1016/j.ympev.2016.11.008

Cited by 26 publications

(8 citation statements)

References 105 publications

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“…Hence, the MFI genome is considered as a suitable candidate for studying systems biological characterization of rumen methanogens. Such systems-level information is currently useful to discover new veterinary vaccines and chemogenomic targets for new CH 4 mitigation interventions ( Bharathi and Chellapandi, 2017 , Sedano-Núñez, Boeren, Stams and Plugge, 2018 ).…”

Section: Methanobacterium Formicicummentioning

confidence: 99%

Methanobacterium formicicum as a target rumen methanogen for the development of new methane mitigation interventions: A review

Chellapandi

Bharathi

Sangavai

et al. 2018

Veterinary and Animal Science

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Methanobacterium formicicum (Methanobacteriaceae family) is an endosymbiotic methanogenic Archaean found in the digestive tracts of ruminants and elsewhere. It has been significantly implicated in global CH 4 emission during enteric fermentation processes. In this review, we discuss current genomic and metabolic aspects of this microorganism for the purpose of the discovery of novel veterinary therapeutics. This microorganism encompasses a typical H 2 scavenging system, which facilitates a metabolic symbiosis across the H 2 producing cellulolytic bacteria and fumarate reducing bacteria. To date, five genome-scale metabolic models (iAF692, iMG746, iMB745, iVS941 and iMM518) have been developed. These metabolic reconstructions revealed the cellular and metabolic behaviors of methanogenic archaea. The characteristics of its symbiotic behavior and metabolic crosstalk with competitive rumen anaerobes support understanding of the physiological function and metabolic fate of shared metabolites in the rumen ecosystem. Thus, systems biological characterization of this microorganism may provide a new insight to realize its metabolic significance for the development of a healthy microbiota in ruminants. An in-depth knowledge of this microorganism may allow us to ensure a long term sustainability of ruminant-based agriculture.

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Section: Methanobacterium Formicicummentioning

confidence: 99%

Methanobacterium formicicum as a target rumen methanogen for the development of new methane mitigation interventions: A review

Chellapandi

Bharathi

Sangavai

et al. 2018

Veterinary and Animal Science

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“…These assemblages reflect the different distributions of each methanogen genus, which is likely influenced by body temperature (Figures S18 & S19). Given that members of both methanogen clades are hydrogenotrophs (29) , possess adhesins for attachment to organic surfaces (41,47) , and share many homologs via horizontal gene transfer and shared common ancestry (37) , body temperature may be a main niche axis partitioning these two clades.…”

Section: Discussionmentioning

confidence: 99%

“…First, some Methanothermobacter isolates can grow at 37°C, but not optimally (33). Second, Methanothermobacter has been observed in a number of animals including salamanders (34), trout (35), chickens (36), buffalo (37) but very sparsely and at low abundances. Third, we found Methanothermobacter abundances to correlate with higher body temperatures of avian and some mammal species which have generally been less-studied, especially in regards to archaeal diversity (Figures S18 & S19).…”

Section: Discussionmentioning

confidence: 99%

Strong influence of vertebrate host phylogeny on gut archaeal diversity

Youngblut

Reischer

Dauser

et al. 2020

Preprint

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Commonly used 16S rRNA gene primers miss much of the archaeal diversity present in the vertebrate gut, leaving open the question of which archaea are host associated, the specificities of such associations, and the major factors influencing archaeal diversity. We applied 16S rRNA amplicon sequencing with Archaea-targeting primers to a dataset of 311 fecal/gut samples spanning 5 taxonomic classes (Mammalia, Aves, Reptilia, Amphibia, and Actinopterygii) and obtained from mainly wild individuals (76% were wild). We obtained sufficient archaeal sequence data from 185 samples comprising 110 species that span all 5 classes. We provide evidence for novel Archaea-host associations, including Bathyarchaeia and Methanothermobacter — the latter of which was prevalent among Aves and enriched in higher body temperatures. Host phylogeny more strongly explained archaeal diversity than diet, while specific taxa were associated with each factor. Co-phylogeny was significant and strongest for mammalian herbivores. Methanobacteria was the only class predicted to be present in the last command ancestors of mammals and all host species. Archaea-Bacteria interactions seem to have a limited effect on archaeal diversity. These findings substantially expand on the paradigm of Archaea-vertebrate associations and the factors that explain those associations.SignificanceArchaea play key roles in the vertebrate gut such as promoting bacterial fermentation via consumption of waste products. Moreover, gut-inhabiting methanogenic Archaea in livestock are a substantial source of greenhouse gas production. Still, much is not known of the archaeal diversity in most vertebrates, especially since 16S rRNA sequence surveys often miss much of the archaeal diversity that is present. By applying Archaea-targeted gut microbiome sequencing to a large collection of diverse vertebrates, we reveal new Archaea-host associations such as a high prevalence of Methanothermobacter in birds. We also show that host evolutionary history explains archaeal diversity better than diet, and certain genera in one particular class of Archaea (Methanobacteria) were likely pervasive in the ancestral vertebrate gut.

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“…This could be attributed to the inhibition of specific methanogens present in the rumen which are known to synthesize their cell membrane via mevalonate, for instance, M. ruminantium (Bharathi and Chellapandi, 2017). Moreover, it has been reported that Lv completely inhibited CH 4 production of the strain (DemonfortNkamga et al, 2017).…”

Section: Effect Of Lovastatin Concentration On Total Gas Production Ch 4 Hydrogen and Short-chain Fatty Acidsmentioning

confidence: 99%

Effects of Fermented Oat Straw as a Lovastatin Carrier on in vitro Methane Production and Rumen Microbiota

et al. 2021

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To date, there is an urgent need for implementing practical strategies to reduce CH4 emissions from ruminants. Lovastatin (Lv) is a specific inhibitor of methanogenic archaea. Due to the high cost of pure Lv, solid-state fermentation might be an economical bioprocess to produce Lv and facilitate its use in ruminant nutrition. The goal of this work was to assess the effects of supplementing fermented oat straw as a lovastatin carrier (FOS) to a high-grain ration on in vitro CH4 inhibition and rumen microbiota in beef cattle. The experimental design of in vitro rumen fermentation was completely randomized with four concentrations of Lv in the diet mixture. The supplementation with FOS to give Lv concentration of 100 and 150 mg L−1 in the ruminal fermentation medium significantly inhibited methanogenesis at similar levels. This suggested that less than 20% of FOS was required in the ration to achieve up to 38% of CH4 mitigation without affecting the chemical composition and nutritional value of the ration. Short-chain fatty acid (SCFA) production and profile showed that only the treatments with Lv at 100 and 150 mg L−1 decreased the concentration of total SCFAs; the molar ratio of propionate significantly increased with respect to that of the control. Treatment with Lv at 150 mg L−1 did not result in significant differences in the alpha and beta diversity indices compared to the control. However, significant changes in the relative abundance of some microorganisms were detected, such as an increase in Ruminococcus and a decrease in Prevotella. The predominant 99%+ MA in all controls, treatment, and inocula samples belonged to the Methanobrevibacter genus and very small (negligible) unclassified Methanobacterium genus (Euryarchaeota phylum). Interestingly, the reduction of relative abundance of MA was 39.17%, very close to the percent reduction of CH4 production, 38%. Our data showed that there was a parallel and similar percent decrease of both CH4 production and relative abundance of the predominant MA in our experiment, although the statistical significance was not complete. Finally, our results hold promise for significantly decreasing ruminal CH4 by 38%. Thus, our work is one step toward the sustainable management of the livestock sector.

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Intergenomic evolution and metabolic cross-talk between rumen and thermophilic autotrophic methanogenic archaea

Cited by 26 publications

References 105 publications

Methanobacterium formicicum as a target rumen methanogen for the development of new methane mitigation interventions: A review

Methanobacterium formicicum as a target rumen methanogen for the development of new methane mitigation interventions: A review

Strong influence of vertebrate host phylogeny on gut archaeal diversity

Effects of Fermented Oat Straw as a Lovastatin Carrier on in vitro Methane Production and Rumen Microbiota

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