Intergenotypic variation of nitric oxide and inflammatory markers in preeclampsia: A pilot study in a North Indian population

Sharma, Deepika; Singh, Archana; Trivedi, Shubha Sagar; Bhattacharjee, Jayashree

doi:10.1016/j.humimm.2011.02.007

Cited by 20 publications

(7 citation statements)

References 30 publications

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“…High levels of inflammatory factors in non-pregnant HIV-infected adults were previously shown to correlate with increased risk of non-AIDS severe adverse events, such as cardiovascular, hepatic and renal dysfunctions [63]. In pregnancy, high inflammatory factors may lead to obstetric morbid conditions, such as premature delivery, pre-eclampsia and liver disorders [38], [64], [65], [66]. In HIV-infected pregnant women, like in other HIV-infected non-pregnant adults [8], [9], inflammatory factors, Th1, Th2 and Treg cytokines were positively correlated with Treg%, probably reflecting a common pathophysiologic mechanism.…”

Section: Discussionmentioning

confidence: 99%

Dynamics of Regulatory T-Cells during Pregnancy: Effect of HIV Infection and Correlations with Other Immune Parameters

Richardson

Weinberg

2011

PLoS ONE

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ObjectivesRegulatory T cells (Treg) increase in the context of HIV infection and pregnancy. We studied Treg subpopulations in HIV-infected and uninfected women during pregnancy and their relationship with inflammation, activation and cell-mediated immunity (CMI).Design and MethodsBlood obtained from 20 HIV-infected and 18 uninfected women during early and late gestation was used to measure Treg and activated T cells (Tact) by flow cytometry; plasma cytokines and inflammatory markers by ELISA and chemoluminescence; and CMI against varicella-zoster virus (VZV) by lymphocyte proliferation.Results and ConclusionsCompared with uninfected women, HIV-infected participants had higher frequencies of Treg subpopulations in early pregnancy, including CD4+CD25+FoxP3+%, CD8+CD25+FoxP3+%, CD4+TGFβ+% and CD4+IL10+%. In contrast, Treg frequencies were lower during late pregnancy in HIV-infected compared with uninfected women, including CD8+TGFβ+%, CD4+CTLA4+% and CD8+CTLA4+%. VZV-CMI, which was lower in HIV-infected compared with uninfected pregnant women, was inversely correlated with CD4+FoxP3+%, CD8+FoxP3+% and CD8+TGFβ+% in HIV-infected, but not in uninfected pregnant women. β2-microglobulin, neopterin, IL1, IL4, IL8, IL10, IFNγ and TNFα plasma concentrations as well as Tact were higher in HIV-infected compared with uninfected women throughout pregnancy. In HIV-infected, but not in uninfected women, inflammatory, Th1, Th2 and regulatory cytokines increased with higher Treg%, suggesting that inflammation and regulation have a common pathophysiologic origin in the context of HIV infection. In HIV-infected and more commonly in uninfected pregnant women, higher Treg% correlated with lower Tact%. We conclude that Treg have different dynamics during pregnancy in HIV-infected and uninfected women. Higher levels of inflammatory cytokines and lower Treg% during late pregnancy in HIV-infected women may contribute to their increased incidence of maternal-fetal morbidity.

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Section: Discussionmentioning

confidence: 99%

Dynamics of Regulatory T-Cells during Pregnancy: Effect of HIV Infection and Correlations with Other Immune Parameters

Richardson

Weinberg

2011

PLoS ONE

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“…PE is thought to be a multifactorial disease that involves oxidative stress, endothelial dysfunction, inflammation, and immunity [3][4][5]. Previous studies of PE have shown interactions between inflammation and endothelial dysfunction [6] and between inflammation and oxidative stress [7]. We also previously found a relationship between oxidative stress and immunity in placental trophoblasts in PE [8].…”

Section: Introductionmentioning

confidence: 89%

A Role of sFlt-1 in Oxidative Stress and Apoptosis in Human and Mouse Pre-Eclamptic Trophoblasts1

Jiang

Zou

et al. 2015

Biology of Reproduction

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Pre-eclampsia (PE) is a hypertensive disorder that occurs during pregnancy, and is a multifactorial disease. The antiangiogenic factor, soluble fms-like tyrosine kinase 1 (sFlt-1), has been reported to be important in the pathogenesis of PE, but the mechanism of its involvement remains unknown. To test the effects of sFlt-1 on pregnancy, we injected pregnant mice with exogenous mouse sFlt-1. After 18 days of gestation, higher blood pressure, proteinuria, and histological differences were observed compared with controls. Mitochondrial swelling inside the trophoblast cells in the placenta of sFlt-1-treated pregnant mice was observed by electron microscopy, which suggested a role of sFlt-1 in oxidative stress in trophoblasts in PE. Furthermore, apoptosis markers were upregulated in sFlt-1-treated mice. In conclusion, sFlt-1 appears to play a role in oxidative stress, which promotes apoptosis of trophoblasts. This may be an important mechanism in the development of PE.

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“…1 ). Of the publications that were considered to be possibly relevant for the analysis, the following were excluded: two duplicate publications 24 25 , one study 26 that included controls with hypertension in pregnancy, one study with unreliable data 27 , three studies 28 29 30 with controls not in the Hardy-Weinberg equilibrium (HWE), and one study with insufficient data to calculate HWE 31 . Finally, 41case-control studies, including 5,211 cases and 8,779 controls, were used to evaluate the associations of NOS3 polymorphisms (G894T, T-786C, and VNTR 4b/a) with the risk for preeclampsia ( Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

Associations between nitric oxide synthase 3 gene polymorphisms and preeclampsia risk: a meta-analysis

Zeng

Zhu

Wong

et al. 2016

Sci Rep

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Previous studies have examined the role of three NOS3 gene polymorphisms [G894T, T-786C, and the variable number of tandem repeats 4b/a (VNTR 4b/a)] in the susceptibility to preeclampsia with inconclusive findings. We therefore conducted an updated meta-analysis by including more studies. The most appropriate genetic model was chosen for each polymorphism by using a well-established method. Pooled results indicated that, compared with the GT + GG genotype, the TT genotype of G894T was associated with an increased risk of preeclampsia (odds ratio (OR) = 1.46; 95% confidence interval (CI) = 1.21–1.77, P < 0.001; I2 = 40.2%). The CC genotype of T-786C was also associated with a higher risk of preeclampsia (OR = 1.30; 95% CI = 1.07–1.58, P = 0.034; I2 = 46.9%) than the CT + TT genotype. No association was found for VNTR 4b/a. Stratified analysis indicated that the increased risk was evident for high-quality studies both for G894T and T-786C, and for studies conducted among Caucasians and Africans for T-786C. However, the increased risk for T-786C among Africans needs further confirmation due to the high probability of false-positive reports. Our results suggested that G894T and T-786C polymorphisms, but not VNTR 4b/a, were associated with an increased risk of preeclampsia.

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Intergenotypic variation of nitric oxide and inflammatory markers in preeclampsia: A pilot study in a North Indian population

Cited by 20 publications

References 30 publications

Dynamics of Regulatory T-Cells during Pregnancy: Effect of HIV Infection and Correlations with Other Immune Parameters

Dynamics of Regulatory T-Cells during Pregnancy: Effect of HIV Infection and Correlations with Other Immune Parameters

A Role of sFlt-1 in Oxidative Stress and Apoptosis in Human and Mouse Pre-Eclamptic Trophoblasts1

Associations between nitric oxide synthase 3 gene polymorphisms and preeclampsia risk: a meta-analysis

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