Interlaboratory comparison of ability to detect nucleic acid of<i>Mycoplasma gallisepticum</i>and<i>Mycoplasma synoviae</i>by polymerase chain reaction

Heß, Michael; Neubauer, C.; Hackl, R.

doi:10.1080/03079450701203082

Cited by 22 publications

(16 citation statements)

References 23 publications

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“…It causes chronic respiratory disease (CRD) in chickens and other fowl (9). Mycoplasma gallisepticum can be found of sera from cases of CRD infection for the presence of antibodies to M. gallisepticum (6,11), as well as molecular detection of M. gallisepticum (13,31,36).…”

Section: Introductionmentioning

confidence: 99%

Mycoplasma gallisepticum: an emerging challenge to the poultry industry in Egypt

Osman¹,

Aly²,

Amin³

et al. 2009

Rev. Sci. Tech. OIE

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In this study, the authors examined the technical performance of culture methodology using specific media: Mycoplasma isolation media of pleuropneumonia-like organisms (PPLO) broth and PPLO agar. Digitonin sensitivity, growth inhibition, the serum plate agglutination test, a commercially available enzyme-linked immunosorbent assay (ELISA) and a commercially available simplex polymerase chain reaction (PCR) test were used to detect Mycoplasma gallisepticum infections in samples collected from the lungs, trachea and tracheal swabs of poultry. These samples were collected from broiler-breeder flocks, broiler flocks and layer flocks. In addition, genomic bacterial deoxyribonucleic acid (DNA) was extracted and amplified, using a simplex PCR. The seroprevalence of M. gallisepticum antibodies in chickens and chicks was also investigated. The prevalence of M. gallisepticum was found to be highest in the layer flocks, at 33.3% (17/51), when the tracheal swab procedure was adopted. In young birds, the serum plate agglutination test and ELISA assay detected antibodies against M. gallisepticum in 69.9% (320/458) and 58.3% (267/458) of the chicken samples, respectively, and 48.7% (146/300) and 60% (180/300) of the samples from the chicks.

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Section: Introductionmentioning

confidence: 99%

Mycoplasma gallisepticum: an emerging challenge to the poultry industry in Egypt

Osman¹,

Aly²,

Amin³

et al. 2009

Rev. Sci. Tech. OIE

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“…Garcia et al [13] compared the 16S rRNA, mgc2, lipoprotein and gapA surface protein genes for MG detection and found that mgc2 and the 16S rRNA methods had similar and the best detection limits. Hess et al [14] reported that MG 16S rRNA genebased PCR, which was developed by Lauermann [12] , had higher analytical sensitivity than other PCR methods tested. It has been reported that the 16S rRNA-based PCR can amplify DNA from Mycoplasma imitans (MI), a phylogenetically related avian Mycoplasma with very similar Detection of Mycoplasma ...…”

Section: Mg and Ms Rpcrsmentioning

confidence: 99%

Damızlık Tavuk Kümeslerinde Mycoplasma gallisepticum ve Mycoplasma synoviae’nin Gerçek Zamanlı PCR’lar ile ve Mycoplasma gallisepticum Antikorlarının ELISA ile Tespiti

Kahya¹,

Yılmaz²,

Eyigör³

et al. 2015

Kafkas Univ Vet Fak Derg

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This study aimed to determine the prevalence of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) in breeder flocks showing respiratory symptoms. A total of 77 flocks (2153 tracheal swabs and blood samples) were sampled and all were tested by MG real time PCR (MG-rPCR) and MG-ELISA, and 32 flocks were tested by MS real time PCR (MS-rPCR). In the first part of this study covering 28 flocks, all samples from chickens with marked clinical symptoms and high MG-antibody levels gave negative results with MG-rPCR1. Therefore, the MG-lipoprotein gene-specific primers (MG-rPCR1) of this PCR were replaced with MG-16S rRNA primers (MG-rPCR2), as were the MS-16S rRNA primers (MS-rPCR), thus the study was pursued accordingly. All of the first 28 flocks, which were 100% positive by MG-ELISA, were MG-rPCR1 negative, whereas in the second part of the study, other 49 flocks, which were 87.8% MG seropositive, were found 42.9% positive by MG-rPCR2. In addition, 5 selected flocks from the first 28 were negative, whereas 7.4% of the 27 selected flocks from the second 49 were positive by MS-rPCR. Overall, 81 out of 432 MG-rPCR1-2 (18.7%) performed from 77 flocks, and 13 out of 187 MS-rPCRs (6.9%) in 32 flocks were determined as positive. ELISA results indicated that there could be significantly high false-positives in serological tests, thus results should not be relied upon one test system. Also, this study revealed that, for the confirmation of Mycoplasma-infected flocks in laboratories, rPCR is a reliable method as long as suitable primers are selected, and that MG and MS prevalence is considerably high in winter season. Keywords

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“…Son sensibles, específicas, rápidas y robustas y, además permiten su robotización y automatización, lo que facilita el análisis de un número elevado de muestras. Los usos de la técnica son muy variados, en sanidad animal la PCR ha sido muy utili-a154 zada para la detección de agentes patógenos de difí-cil aislamiento o crecimiento lento, como los Mycoplasmas aviares (Hess, Neubauer y Hackl, 2007), y en aquellos casos de infecciones crónicas o persistentes en los que no hay serología y los animales infectados son reservorio del patógeno, como es el caso del virus de la diarrea viral bovina (BVD) (Lanyon et al, 2014).…”

Section: Diagnóstico Molecularunclassified

La biotecnología en sanidad animal

García

Casares

Moran

et al. 2014

Arbor

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RESUMEN: El crecimiento de la población mundial en los próxi-mos años, provocará un incremento en la demanda de alimentos. Como consecuencia, el número de animales de producción sufrirá un aumento importante y la Sanidad Animal constituirá un elemento crítico a la hora de tratar y prevenir las enfermedades, garantizando el abastecimiento y la seguridad. La biotecnología contribuye de múltiples formas al desarrollo de la Sanidad Animal. Fundamentalmente, aporta herramientas que ayudan al control y erradicación de las enfermedades. El desarrollo de la ingeniería genética en las últimas décadas ha favorecido el avance de la industria biotecnológica aplicada a la sanidad humana y animal. Existen un gran número de ensayos de diagnóstico basados en desarrollos biotecnológicos, así mismo, cada vez hay más vacunas recombinantes contra enfermedades animales. La prevención y control eficientes de las enfermedades dependerán de mecanismos de detección temprana, que faciliten la toma de decisiones y una respuesta rápida. PALABRAS CLAVE:Vacunación; VLPs; diagnóstico; inmunoensayos; DIVA; ensayos múltiples; ensayos de campo; PCR.ABSTRACT: The growth of the world population in forthcoming years will cause an increase in the demand of food. As a result, the number of working animals is set to rise significantly and Animal Health will become a vital issue when it comes to treating and preventing diseases, and guaranteeing and uninterrupted and safe food supply. Biotechnology contributes to the development of Animal Health in many ways. First and foremost, it provides tools that help control and eradicate diseases. The progress of genetic engineering in recent decades has underpinned the development of the biotechnology industry applied to human and animal health. There are a large number of diagnostic tests based on biotechnological developments, and there is also an increase in the use of recombinant vaccines against animal diseases. An effective prevention and control of diseases will depend on early detection mechanisms that facilitate decisionmaking and enable a rapid response. KEYWORDS:Vaccination; VLPs; diagnostic; immunoassays; DIVA; multiplex assays; field tests; PCR.

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Interlaboratory comparison of ability to detect nucleic acid ofMycoplasma gallisepticumandMycoplasma synoviaeby polymerase chain reaction

Cited by 22 publications

References 23 publications

Mycoplasma gallisepticum: an emerging challenge to the poultry industry in Egypt

Mycoplasma gallisepticum: an emerging challenge to the poultry industry in Egypt

Damızlık Tavuk Kümeslerinde Mycoplasma gallisepticum ve Mycoplasma synoviae’nin Gerçek Zamanlı PCR’lar ile ve Mycoplasma gallisepticum Antikorlarının ELISA ile Tespiti

La biotecnología en sanidad animal

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