In order to demonstrate the performance of a retrofitted selective catalytic reduction (SCR) system while also addressing the issues associated with greater use of biodiesel, a 2005 International 9200i tractor owned by the City of Santa Monica was retrofitted with a titaniavanadia-tungsten catalyst and a urea dosing system supplied by Extengine Systems, Inc. This tractor was operated under normal service conditions within the City of Santa Monica refuse collection and transportation fleet. An on-board emissions measurement system supplied by Engine, Fuel, and Emissions Engineering, Inc., was installed on the vehicle; it measured the emissions and fuel use of the vehicle while it operated on ultra-low-sulfur diesel (ULSD), 20% biodiesel (B20), and 99% biodiesel (B99) on consecutive days. The vehicle, with a similar aftertreatment system, was then tested at the California Air Resources Board (CARB) Los Angeles Heavy-Duty Emissions Lab and tested on the Urban Dynamometer Driving Schedule (UDDS) to again assess the effects of ULSD, B20, and B99 on the performance of the urea-SCR aftertreatment system.Results from this testing showed that oxides of nitrogen (NO x ) emissions increased by 6%-12% when the blend was increased from ULSD to B20, by 16%-35% when the blend was increased from ULSD to B99, and by 26%-27% when it was increased from B20 to B99. Particulate matter (PM) emissions decreased by 37%-50% when the blend was increased from ULSD to B20, by 71%-79% when it was increased from ULSD to B99, and by 60%-63% when it was increased from B20 to B99. The SCR system reduced NO x emissions by 64%-87%, and its performance was not affected by the use of biodiesel.