Interlaboratory round robin on cantilever calibration for AFM force spectroscopy

Riet, Joost te; Katan, Allard J.; Rankl, Christian; Ståhl, S.; Buul, Arend M. van; Phang, In Yee; Gómez-Casado, Alberto; Schön, Peter; Gerritsen, Jan W.; Cambi, Alessandra; Rowan, Alan E.; Vancso, Gyula J.; Jonkheijm, Pascal; Huskens, Jurriaan; Oosterkamp, Tjerk H.; Gaub, Hermann E.; Hinterdorfer, Peter; Figdor, Carl G.; Speller, S.

doi:10.1016/j.ultramic.2011.09.012

Cited by 110 publications

(88 citation statements)

References 54 publications

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“…This method has an accuracy in the range of 6-15% [26]. The main cause for the error is that this method suffers from systematic errors in determining the correct deflection sensitivity [26].…”

Section: Numerical Simulations 31 Numerical Modelmentioning

confidence: 99%

“…The main cause for the error is that this method suffers from systematic errors in determining the correct deflection sensitivity [26]. Since there is a linear relationship between the elastic modulus E of the cantilever and the spring constant k (Section 3.2), and the value of E used in the numerical model was calculated directly from k (Section 3.2), this error is not included in the comparison of the experiments with the numerical results.…”

Section: Numerical Simulations 31 Numerical Modelmentioning

confidence: 99%

See 1 more Smart Citation

Numerical study of the hydrodynamic drag force in atomic force microscopy measurements undertaken in fluids

Méndez-Méndez

Alonso-Rasgado

Faria

et al. 2014

Micron

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When atomic force microscopy (AFM) is employed for in vivo study of immersed biological samples, the fluid medium presents additional complexities, not least of which is the hydrodynamic drag force due to viscous friction of the cantilever with the liquid. This force should be considered when interpreting experimental results and any calculated material properties. In this paper, a numerical model is presented to study the influence of the drag force on experimental data obtained from AFM measurements using computational fluid dynamics (CFD) simulation. The model provides quantification of the drag force in AFM measurements of soft specimens in fluids.The numerical predictions were compared with experimental data obtained using AFM with a V-shaped cantilever fitted with a pyramidal tip. Tip velocities ranging from 1.05 to 105 µm/s were employed in water, polyethylene glycol and glycerol with the platform approaching from a distance of 6000 nm. The model was also compared with an existing analytical model. Good agreement was observed between numerical results, experiments and analytical predictions. Accurate predictions were obtained without the need for extrapolation of experimental data. In addition, the model can be employed over the range of tip geometries and velocities typically utilized in AFM measurements.

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Section: Numerical Simulations 31 Numerical Modelmentioning

confidence: 99%

Section: Numerical Simulations 31 Numerical Modelmentioning

confidence: 99%

Numerical study of the hydrodynamic drag force in atomic force microscopy measurements undertaken in fluids

Méndez-Méndez

Alonso-Rasgado

Faria

et al. 2014

Micron

View full text Add to dashboard Cite

show abstract

“…Based on early reports of these calibration methods and more recent research that include an inter-laboratory study [1][2][3][4][5][6][7], error estimates for their operation have been formulated. Such (averaged) estimates are typically used to quantify the accuracy of individual force measurements, but do not account for the variation of measurement uncertainty between different users and laboratories.…”

Section: Introductionmentioning

confidence: 99%

“…While applicable to any cantilever, spring constant measurements from this method are known to depend on several factors including the laser spot size and position, z-displacement piezo calibration, static-to-dynamic optical lever sensitivity and nonlinearity in the deflection curve on hard contact [1][2][3][4][5][6]9, 10]. Thus, variations in calibration accuracy can easily emerge between laboratories and even different users within a single laboratory.…”

Section: Introductionmentioning

confidence: 99%

A virtual instrument to standardise the calibration of atomic force microscope cantilevers

Sader

Borgani

Gibson

et al. 2016

Review of Scientific Instruments

135

105

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Atomic force microscope (AFM) users often calibrate the spring constants of cantilevers using functionality built into individual instruments. This is performed without reference to a global standard, which hinders robust comparison of force measurements reported by different laboratories. In this article, we describe a virtual instrument (an internet-based initiative) whereby users from all laboratories can instantly and quantitatively compare their calibration measurements to those of others -standardising AFM force measurements -and simultaneously enabling noninvasive calibration of AFM cantilevers of any geometry. This global calibration initiative requires no additional instrumentation or data processing on the part of the user. It utilises a single website where users upload currently available data. A proof-of-principle demonstration of this initiative is presented using measured data from five independent laboratories across three countries, which also allows for an assessment of current calibration.

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“…Many calibration methods exist for finding the spring constant at the first or fundamental mode 13 . They typically seek to avoid using properties of a cantilever that are difficult to measure, such as thickness or density.…”

mentioning

confidence: 99%

A calibration method for the higher modes of a micro-mechanical cantilever

Shatil

Homer

Picco

et al. 2017

Applied Physics Letters

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General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/pure/about/ebr-terms A Calibration Method for the Higher Modes of a Micro-mechanical Cantilever Micro-mechanical cantilevers are increasingly being used as a characterisation tool in both material and biological sciences. New non-destructive applications are being developed that rely on the information encoded within the cantilever's higher oscillatory modes, such as AFM techniques that measure non-topographic properties of a sample. However, these methods require the spring constants of the cantilever at higher modes to be known in order to quantify their results. Here, we show how to calibrate the micro-mechanical cantilever and find the effective spring constant of any mode. The method is uncomplicated to implement, using only properties of the cantilever and the fundamental mode that are straightforward to measure.

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Interlaboratory round robin on cantilever calibration for AFM force spectroscopy

Cited by 110 publications

References 54 publications

Numerical study of the hydrodynamic drag force in atomic force microscopy measurements undertaken in fluids

Numerical study of the hydrodynamic drag force in atomic force microscopy measurements undertaken in fluids

A virtual instrument to standardise the calibration of atomic force microscope cantilevers

A calibration method for the higher modes of a micro-mechanical cantilever

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