Interlaboratory study of a supercritical fluid chromatography method for the determination of pharmaceutical impurities: Evaluation of multi-systems reproducibility

Dispas, Amandine; Clarke, Adrian; Perrenoud, Alexandre Grand-Guillaume; Losacco, Luca Gioacchino; Veuthey, Jean‐Luc; Gros, Quentin; Molineau, Jérémy; Noireau, Angéline; West, Caroline; Salafia, Fabio; Zoccali, Mariosimone; Mondello, Luigi; Guillen, Amber; Wang, Jenny; Kelly, Zhang; Jochems, Philipp; Schad, Gesa; Nakajima, Kiyokazu; Horie, Shinnosuke; Joseph, Jan Felix; Parr, Maria Kristina; Billemont, Pierre; Severino, Antoni; Schneider, Sonja; Naegele, Edgar; Kutscher, Daniel; Wikfors, Rick; Black, Regina M.; Ingvaldson, Lee; Silva, Jimmy Oliveira Da; Bennett, Raffeal; Regalado, Erik L.; Hoang, Thi Phuong Thuy; Touboul, David; Nikolova, Yana; Kamenova-Nacheva, Mariana; Dimitrov, Vladimir; Berger, Blair K.; Schug, Kevin A.; Kerviel-Guillon, Solène; Mauge, Fabien; Takahashi, Masatomo; Izumi, Yoshihiro; Bamba, Takeshi; Rouvière, Florent; Heinisch, Sabine; Guillarme, Davy; Hubert, Philippe

doi:10.1016/j.jpba.2021.114206

Cited by 20 publications

(1 citation statement)

References 8 publications

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“…However, this technique is still primarily considered a research tool rather than a routine method, even though the causes of the technique’s negative reputation, including a lack of method robustness, instrument unreliability, and complex technology transfer, were already mitigated . In recent years, several studies on interlaboratory validation have been carried out confirming the robustness and repeatability of SFC methods. − Nevertheless, several negative aspects related to long-term retention time ( t R ) stability have also been described for SFC. , …”

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Advancing Fundamental Understanding of Retention Interactions in Supercritical Fluid Chromatography Using Artificial Neural Networks: Polar Stationary Phases with –OH Moieties

Plachká,

Pilařová,

Gazárková

et al. 2024

Anal. Chem.

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The retention behavior in supercritical fluid chromatography and its stability over time are still unsatisfactorily explained phenomena despite many important contributions in recent years, especially focusing on linear solvation energy relationship modeling. We studied polar stationary phases with predominant −OH functionalities, i.e., silica, hybrid silica, and diol columns, and their retention behavior over time. We correlated molecular descriptors of analytes with their retention using three organic modifiers of the CO 2 -based mobile phase. The differences in retention behavior caused by using additives, namely, 10 mmol/L NH 3 and 2% H 2 O in methanol, were described in correlation to analyte properties and compared with the CO 2 /methanol mobile phase. The structure of >100 molecules included in this study was optimized by semiempirical AM1 quantum mechanical calculations and subsequently described by 226 molecular descriptors including topological, constitutional, hybrid, electronic, and geometric descriptors. An artificial neural networks simulator with deep learning toolbox was trained on this extensive set of experimental data and subsequently used to determine key molecular descriptors affecting the retention by the highest extent. After comprehensive statistical analysis of the experimental data collected during one year of column use, the retention on different stationary phases was fundamentally described. The changes in the retention behavior during one year of column use were described and their explanation with a proposed interpretation of changes on the stationary phase surface was suggested. The effect of the regeneration procedure on the retention was also evaluated. This fundamental understanding of interactions responsible for retention in SFC can be used for the evidence-based selection of stationary phases suitable for the separation of particular analytes based on their specific physicochemical properties.

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