Interleukin-17 mediates inflammatory tissue injury during orf development in goats

Tang, Xidian; Tian, Jing; Chen, Xi; Wang, Tianxing; Chen, Fengqiang; Wen, Yiping; Chang, Jianjun; Chen, Dekun; Ma, Wentao

doi:10.1016/j.vetmic.2021.109105

Cited by 6 publications

(3 citation statements)

References 43 publications

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“…After hematoxylin and eosin staining, tissue slices were observed and analyzed under an optical microscope. Refer to previous research for histopathological scoring ( 56 ). Briefly, the histopathological scoring was performed on a scale of 0–3: no inflammatory cell infiltration, with basic intact tissue structure (0 score), mild infiltration of inflammatory cells and slight changes in tissue structure (1 score), moderate infiltration of inflammatory cells and moderate changes in tissue structure (2 score), inflammatory cells infiltrate largely and extensively, with severe changes in tissue structure (3 score).…”

Section: Methodsmentioning

confidence: 99%

Lactobacillus acidophilus protects against Corynebacterium pseudotuberculosis infection by regulating the autophagy of macrophages and maintaining gut microbiota homeostasis in C57BL/6 mice

Li,

Jiang,

Cui

et al. 2024

mSystems

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Corynebacterium pseudotuberculosis ( C. p ), a facultative intracellular bacterium, is an important zoonotic pathogen that causes abscesses and pyogenic granulomas. The relationship between gut microbiota and host health or diseases has received increasing attention. However, the role of gut microbiota in the process of C. p infection is still unclear. In this study, we established a C. p infection model in C57BL/6 mice and examined the impact of preemptive oral administration Lactobacillus acidophilus ( L. acidophilus ) on infection. Our findings revealed that C. p infection led to pronounced pathological alterations in the liver and kidneys, characterized by abscess formation, intense inflammatory responses, and bacterial overload. Remarkably, these deleterious effects were greatly relieved by oral administration of L. acidophilus before infection with C. p . Additionally, we further found that during C. p infection, peritoneal macrophages (PMs) of mice orally administered with L. acidophilus accumulated more rapidly at sites of infection. Furthermore, our results showed that PMs from mice with oral L. acidophilus administration showed a stronger C. p clearance effect, and this was mediated by high expression of LC3-II protein. Meanwhile, oral administration of L. acidophilus protected the gut microbiota disorder in C57BL/6 mice caused by C. p infection. In summary, our study demonstrates that oral administration of L. acidophilus confers effective protection against C. p infection in C57BL/6 mice by modulating macrophage autophagy, thereby augmenting bacterial clearance and preserving gut microbiota and function stability. These findings position L. acidophilus as a viable probiotic candidate for the clinical prevention of C. p infection. IMPORTANCE Corynebacterium pseudotuberculosis (C. p ) is known to induce a range of chronic diseases in both animals and humans. Currently, clinical treatment for C. p infection mainly relies on antibiotic therapy or surgical intervention. However, excessive use of antibiotics may increase the risk of drug-resistant strains, and the effectiveness of treatment remains unsatisfactory. Furthermore, surgical procedures do not completely eradicate pathogens and can easily cause environmental pollution. Probiotic interventions are receiving increasing attention for improving the body’s immune system and maintaining health. In this study, we established a C. p infection model in C57BL/6 mice to explore the impact of Lactobacillus acidophilus during C. p infection. Our results showed that L. acidophilus effectively protected against C. p infection by regulating the autophagy of macrophages and maintaining intestinal microbiota homeostasis. This study may provide a new strategy for the prevention of C. p infection.

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Section: Methodsmentioning

confidence: 99%

Lactobacillus acidophilus protects against Corynebacterium pseudotuberculosis infection by regulating the autophagy of macrophages and maintaining gut microbiota homeostasis in C57BL/6 mice

Li,

Jiang,

Cui

et al. 2024

mSystems

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“…GLF were isolated from the lip of goat embryos. The adherent cells were obtained by the same method as previously reported ( 17 ) and were treated with trypsin-EDTA (Thermo Fisher Scientific) for 1 min. When most fibroblasts were detached, they were collected and centrifuged at 800 × g for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Dual analysis of wild-type and attenuated Orf virus and host cell transcriptomes revealed novel virus-host cell interactions

Yao,

Jing,

Geng

et al. 2023

mSphere

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The attenuated Orf virus (ORFV) strain (aFX0910), derived from continuous passages of wild-type strain FX0910 in vitro , displays obviously lower virulence in kids. However, the latent virulence determinants and intracellular mechanisms for the attenuated variant remain unknown. In this study, we constructed a comprehensive analysis of the ORFV and goat lip fibroblasts transcriptomes during viral infection using RNA sequencing. Focusing on the host transcriptome, we performed gene expression analysis, Gene Ontology analysis, and biological pathway analysis, among others. This integrated analysis allowed us to assess potential changes in mRNA expression and single nucleotide polymorphisms (SNPs) in both viral and host genes. In general, the global patterns of immune gene expression induced by different ORFV isolates were mostly shared. However, host transcripts and non-synonymous of viral genes differed between the wild-type and attenuated ORFV strains. Notably, the natural ORFV isolate (FX0910) predominantly triggered an innate immune response in host cells, while aFX0910 downregulated innate immune response and antiviral response. Analysis of viral transcriptomes manifested that FX0910 and aFX0910 had different SNP events in multiple functional genes. Collectively, our findings highlighted novel insights into virus-host interactions and shed light on the molecular basis of ORFV virulence at the genomic level. Furthermore, this study may contribute to the development of targeted intervention strategies and the advancement of vaccine research in the field of ORFV. IMPORTANCE Currently, the only available commercial vaccines for Orf virus (ORFV) are live attenuated vaccines, which present a potential risk of reversion to virulence. Therefore, understanding the pathogenic mechanisms of different virulent strains of ORFV and host immune responses triggered by these viruses is crucial for developing new vaccines and interventions. In this study, we found that the attenuated strain downregulates the host innate immune response and antiviral activity. In addition, we noted that the wild-type strain can induce the immune response pattern centered on interferon-stimulated genes and interferon regulatory factor gene family. We predicted that STAT1 and STAT2 are the main transcription factors upstream of target gene promoters through gene regulatory networks and exert significant regulatory effects on co-expressed genes. Our study elucidated the complex interaction between ORFV strains and host cell immune responses, providing new insights into vaccine research for ORFV.

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“…A total reaction volume of 25 μL containing 12.5 μL of 2 × Taq MasterMix (CWbiotech, Jiangsu, China), 0.5 μL of each primer, 2 μL of DNA template and 9.5 μL of ddH 2 O was used for PCR amplification. The thermal cycling process was the same as we reported before ( Tong et al, 2020 ; Tang et al, 2021 ). PCR products were analyzed by 1.5% agarose gel electrophoresis.…”

Section: Methodsmentioning

confidence: 99%

Orf Virus Detection in the Saliva and Milk of Dairy Goats

Pang

Lei

et al. 2022

Front. Microbiol.

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Orf is a zoonotic and highly contagious disease caused by Orf virus (ORFV) infection. Orf outbreaks in sheep and goats usually lead to high culling rate and mortality in newborn kids and lambs, posing a great threat to the development of goat and sheep industry. Human Orf occurs via direct contact with infected animals or fomites. While this disease is traditionally thought to spread through direct contact, whether other transmission routes exist remains unclear. Herein, we report the detection of ORFV in the saliva and milk of dairy goats without clinical Orf symptoms. Further analyses showed that these ORFV are infectious, as they can induce characteristic cytopathic changes in primary mammary and lip cells. Importantly, these ORFV can induce typical Orf lesions after inoculation in ORFV-free dairy goats. This is the first study showing that live, infectious ORFV can be isolated from the saliva and milk of asymptomatic goats, highlighting novel potential transmission routes of ORFV. These findings provide a novel idea for the prevention and control of Orf spread.

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Interleukin-17 mediates inflammatory tissue injury during orf development in goats

Cited by 6 publications

References 43 publications

Lactobacillus acidophilus protects against Corynebacterium pseudotuberculosis infection by regulating the autophagy of macrophages and maintaining gut microbiota homeostasis in C57BL/6 mice

Lactobacillus acidophilus protects against Corynebacterium pseudotuberculosis infection by regulating the autophagy of macrophages and maintaining gut microbiota homeostasis in C57BL/6 mice

Dual analysis of wild-type and attenuated Orf virus and host cell transcriptomes revealed novel virus-host cell interactions

Orf Virus Detection in the Saliva and Milk of Dairy Goats

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