Interleukin-1β polymorphisms in Colombian patients with autoimmune rheumatic diseases

Abstract

Interleukin-1 beta (IL-1b) exerts a range of inflammatory and immunomodulatory activities that are important in host defense and autoimmune response. The IL-1b gene, located on chromosome 2 (2q13), is polymorphic. The influence of its polymorphism on 355 patients with autoimmune rheumatic diseases was examined. To this effect, 172 patients with rheumatoid arthritis (RA), 114 with systemic lupus erythematosus (SLE), and 69 with primary Sjö gren's syndrome (pSS) were studied. The control group consisted of 392 matched healthy individuals. Genotyping of IL-1b single-nucleotide polymorphisms (SNPs) at positions À511 (C/T) and þ 3953 (C/T) was performed by the polymerase chain reaction-restriction fragment length polymorphism technique. In addition, levels of IL-1b were measured by immunoassay in supernatants of lipopolysaccharide (LPS)-stimulated and nonstimulated peripheral blood monocytes (PBM) obtained from 19 homozygous individuals for the three most common IL-1b likely haplotypes, all belonging to the control group. Allele þ 3953T was protective for SLE (odds ratio (OR) ¼ 0.57, 95% confidence intervals (CI) ¼ 0.34-0.88, P ¼ 0.01) as was the haplotype À511C þ 3953T (OR ¼ 0.43, 95%CI ¼ 0.25-0.74, pc ¼ 0.006). The latter was associated with a lower LPS-stimulated-PBM IL-1b secretion. Results suggest that IL-1b polymorphism influences the susceptibility to acquire SLE in our population. The protective association might be explained by the observed inhibitory effect of IL-1b þ 3953T allele on the secretion of IL-1b under inflammatory circumstances.

PTPN22 C1858T polymorphism in Colombian patients with autoimmune diseases

“…Their clinical and immunological characteristics were systematically recorded as previously reported. 13,14 The study included 263 women and 35 men with RA, 15 their mean age7s.d. was 48.5714.2 years, the mean duration of disease was 1178.1 years, and 80% tested positive for RF.…”

Possible relations between the polymorphisms of the cytokines IL-19, IL-20 and IL-24 and plaque-type psoriasis

“…The protective haplotypes of the HLA complex genes have been shown in several autoimmune diseases like diabetes, rheumatoid arthritis and systemic lupus erythematosus. [48][49][50] Similarly, certain HLA haplotypes have been shown to be protective in psoriasis. [51][52][53] The knowledge about the non-MHC protective genes in psoriasis is insufficient at the present time.…”

O polimorfismo VNTR no gene codificador do antagonista do receptor da interleucina-1 está associado com a doença arterial coronariana

“…Different results were reported on the effect of IL1B (+3953) allele on IL-1β protein production. Some studies showed that an association was seen between IL1B (+3953) and increased plasma levels of IL-1β 9,30 , but others found no affect on IL-1 levels [33][34][35] . No studies are available on the relationship between CAD and polymorphism in the IL1B gene (+3953).…”