Interleukin 5 and granulocyte-macrophage colony-stimulating factor levels in bronchoalveolar lavage fluid in interstitial lung disease

Taniguchi, Hirokazu; Katoh, Shigeki; Kadota, Jun‐ichi; Matsubara, Y; Fukushima, Kiyoyasu; Mukae, Hiroshi; Matsukura, Shigeru; Kohno, Shigeru

doi:10.1183/09031936.00.16595900

Cited by 31 publications

(28 citation statements)

References 28 publications

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“…The values recorded here fall slightly below this range (0–41 pg/ml). We could find few estimations of IL-3 concentrations in BALF in the literature but the concentrations recorded here (0.01–0.24 pg/ml) are in agreement with a report where concentrations of less than 1 pg/ml were typical [28]. Even accounting for our small sample set, it is obvious that the IL-3 concentrations used to supplement incubation media in this study and many others [14, 15] far exceed those detected in both serum and BALF.…”

Section: Discussionsupporting

confidence: 64%

A Comparison of Inflammatory Mediators Released by Basophils of Asthmatic and Control Subjects in Response to High-Affinity IgE Receptor Aggregation

Gilmartin

Tarleton²,

Schuyler³

et al. 2007

Int Arch Allergy Immunol

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Background: In human blood basophils, cross-linking the high-affinity IgE receptor FcΕRI with multivalent antigen activates a signaling pathway leading to secretion of inflammatory mediators and cytokine production. Basophils are known to play an important role in the pathogenesis of asthma but there has been no comprehensive examination of the effectors these cells produce. Here a study of the transcription and release of a selection of chemokines and cytokines from basophils was undertaken. Methods: A Cartesian antibody array provided an effective method of assaying for multiple cytokines and chemokines simultaneously. Results were verified by RT-PCR and ELISA assays. This allowed the comparison of freshly prepared peripheral blood basophil responses to cross-linking of the high-affinity IgE receptor, with and without preincubation with IL-3. Results: Evidence that human blood basophils produce the chemokines MIP-5, eotaxin and GM-CSF was provided by antibody array and RT-PCR analyses. Preincubation with IL-3 enhanced the expression and release of IL-13, IL-8 and mRNA transcripts encoding MIP-5 and GATA2 in basophils from both asthmatic and control subjects. Leptin mRNA transcription, storage and release in basophils are described for the first time. Conclusions: Surveying cytokine and chemokines stored and released by peripheral blood basophils shows that asthmatic and control subjects share similar profiles even when their degranulation responses are distinct. Evidence is provided for the production of leptin, GM-CSF, eotaxin and MIP-5 by peripheral blood basophils. IL-3 preincubation enhances the production and release of IL-8 upon IgE receptor cross-linking.

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Section: Discussionsupporting

confidence: 64%

A Comparison of Inflammatory Mediators Released by Basophils of Asthmatic and Control Subjects in Response to High-Affinity IgE Receptor Aggregation

Gilmartin

Tarleton²,

Schuyler³

et al. 2007

Int Arch Allergy Immunol

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“…Similarly, increased GM-CSF levels were observed in BALF of patients with IPF that was associated with increased eosinophilia [11]. These findings may be explained by the effect of GM-CSF on eosinophil survival or eosinophil-specific recruitment [25], which is reflected by the relationship observed between GM-CSF and eosinophils but not neutrophils.…”

Section: Discussionmentioning

confidence: 95%

“…GM-CSF has the capacity to influence proliferation, differentiation and recruitment of eosinophils and is also shown to affect fibroblasts and endothelial cells, potentially resulting in tissue remodelling and angiogenesis [10]. Increased levels of GM-CSF in BALF were found in patients with interstitial lung disease with eosinophilia [11].…”

mentioning

confidence: 99%

BALF-derived fibroblasts differ from biopsy-derived fibroblasts in systemic sclerosis

Scheja¹,

Larsen²,

Todorova³

et al. 2006

Eur Respir J

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Growth of fibroblasts from bronchoalveolar lavage fluid (BALF) in patients with systemic sclerosis (SSc) has previously been described. The purpose of the present study was to characterise fibroblasts from BALF and bronchial biopsies from SSc patients with alveolitis and from controls, to analyse fibroblast proliferation, migration, stress fibres and proteoglycan production.BALF and bronchial biopsies were collected from 10 patients with SSc and alveolitis and from 15 controls.Outgrowth of fibroblasts was observed from the BALF of four patients, particularly in those with a markedly increased percentage of eosinophils in BALF, but not in any member of the control group. Increased levels of granulocyte-macrophage colony-stimulating factor, correlating with the percentage of eosinophils in BALF, were found in patients when compared with controls. Fibroblasts from BALF showed an elongated, mobile phenotype and increased proteoglycan production compared to the corresponding biopsy fibroblasts.In conclusion, outgrowth of fibroblasts with an altered phenotype is reported from bronchoalveolar lavage fluid in systemic sclerosis patients with alveolitis and an increased percentage of eosinophils in the bronchoalveolar lavage fluid. These findings indicate a possible role for eosinophil-fibroblast interaction in pulmonary fibrosis in systemic sclerosis.

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“…It plays critical roles in recruiting eosinophils, causing degranulation and prolonging eosinophil survival by inhibiting apoptosis (7)(8)(9). Previous investigations have documented increased concentrations of IL-5 in BAL fluid from patients with eosinophilic pneumonia (10)(11)(12). Furthermore, one study demonstrated that pulmonary eosinophilia in mice could be abrogated by treatment with IL-5 antibodies (13).…”

mentioning

confidence: 97%

Elevated Levels of Thymus- and Activation-regulated Chemokine in Bronchoalveolar Lavage Fluid from Patients with Eosinophilic Pneumonia

Miyazaki

Nureki

Fukami

et al. 2002

Am J Respir Crit Care Med

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Thymus- and activation-regulated chemokine (TARC/CCL17) is a lymphocyte-directed CC chemokine, which plays a role in the recruitment of CC chemokine receptor-4 positive T helper 2 (Th2) cells. In this study, we measured concentrations of TARC and Th2 cell-derived cytokines in bronchoalveolar lavage (BAL) fluid, as well as TARC concentrations in serum from patients with eosinophilic pneumonia and other interstitial lung diseases. TARC was significantly elevated in BAL fluids from patients with eosinophilic pneumonia (median, 240 pg/ml), whereas TARC was undetectable (< 7 pg/ml) in most cases of hypersensitivity pneumonitis, sarcoidosis, and idiopathic pulmonary fibrosis, as well as in healthy control subjects. Also, when present, quantities were less than 20 pg/ml. Elevated concentrations of interleukin (IL)-4, IL-5, and IL-13 were also detected in BAL fluid from patients with eosinophilic pneumonia. Interestingly, TARC concentrations in BAL fluids were closely correlated with the concentrations of IL-5 and IL-13. A serial examination showed that elevated TARC in BAL fluid rapidly fell to below detectable limits preceding decreases in IL-5 concentration and eosinophil percentage. Our results, in concordance with previous studies, demonstrate the potential activity of TARC for recruiting Th2 cells to the lungs and suggest a significant role for TARC in the pathogenesis of eosinophilic pneumonia.

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Interleukin 5 and granulocyte-macrophage colony-stimulating factor levels in bronchoalveolar lavage fluid in interstitial lung disease

Cited by 31 publications

References 28 publications

A Comparison of Inflammatory Mediators Released by Basophils of Asthmatic and Control Subjects in Response to High-Affinity IgE Receptor Aggregation

A Comparison of Inflammatory Mediators Released by Basophils of Asthmatic and Control Subjects in Response to High-Affinity IgE Receptor Aggregation

BALF-derived fibroblasts differ from biopsy-derived fibroblasts in systemic sclerosis

Elevated Levels of Thymus- and Activation-regulated Chemokine in Bronchoalveolar Lavage Fluid from Patients with Eosinophilic Pneumonia

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