Interleukin‐5 mRNA in three T‐cell lymphomas with eosinophilia

Abstract

The objective of this controlled pilot study was to determine if mRNA coding for interleukin-5 (IL-5), a cytokine that promotes eosinophil differentiation, growth, and migration, could be detected in three T-cell lymphomas that were infiltrated extensively by eosinophils. To detect mRNA coding for IL-5, we performed an RNA polymerase chain reaction on mRNA extracted from three T-cell lymphomas with eosinophilia and from 29 positive and negative validation controls. Using this procedure, we detected a 293-base pair, IL-5-specific amplification product in the three cases of T-cell lymphoma with eosinophilia and in 11 of 12 positive validation controls, including 10 cases of Hodgkin's disease with eosinophilia. IL-5 mRNA was not detectable in the 17 negative validation controls. This preliminary study suggests that IL-5 mRNA is detectable by polymerase chain reaction in three cases of T-cell lymphoma with eosinophilia.

Anaplastic Large-Cell Lymphoma (KM Lymphoma) with Expression of IL-5 mRIMA and Eosinophilic Invasion

“…[10], B-ALL (IL-3) [11], Sezary syndrome (IL-5) [12] and T-NHL (IL-5) [13,14], In this study, expression of IL-5 mRNA was not detected in HD ( fig. 3), in which the eosinophilic invasion and eosinophilia were not observed.…”

Eosinophilia associated with adult t-cell leukemia: Role of interleukin 5 and granulocyte-macrophage colony-stimulating factor

“…In malignant lymphoma accompanied by eosinophilia, eosinophilopoietic factors are produced by either malignant cells themselves [15,[17][18][19] or normal MNCs [16]. Our in vitro data indicated that GM-CSF was produced by MNCs from all patients, whereas IL-3 and IL-5 was produced by cells from only one (patient 2).…”

“…Several investigators have reported eosinophil-stimulating cytokine involvement in other lymphoid malignancies that are accompanied by eosinophilia. In Hodgkin's disease, for example [13,14], tumor cells [15] or normal MNCs [16] produce IL-5, and T-cell lymphoma cells from patients with eosinophilia secrete different combinations of IL-3, IL-5, and GM-CSF [17,18]. In spite of the high frequency of eosinophilia, the information about its cause in ATL is limited.…”

Increased Blood Clotting, Microvascular Density, and Inflammation in Eotaxin-Secreting Tumors Implanted into Mice

“…30 Moreover, eosinophils infiltrating interleukin-5, gene-transfected tumors do not suppress tumor growth, 31 and infiltration by eosinophils of natural, interleukin-5-secreting human tumors such as lymphoma is not associated with tumor regression. 11 Finally, we note that the in vitro studies that demonstrated an antitumor cytotoxic effect mediated by eosinophils used exceptionally high ratios of eosinophils to tumor cells (10:1 to 100:1) that probably do not accurately reflect the natural conditions in tumors. 16 Consequently, on the basis of our studies, we conclude that eotaxin is unlikely to elicit an effective anti-tumor response by eosinophils or other inflammatory cells in vivo.…”