2017
DOI: 10.1038/s41598-017-12017-y
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Interleukin-6 blockade attenuates lung cancer tissue construction integrated by cancer stem cells

Abstract: In the present study, we successfully generated lung cancer stem cell (CSC)-like cells by introducing a small set of transcription factors into a lung cancer cell line. In addition to properties that are conventionally referred to as CSC properties, the lung induced CSCs exhibited the ability to form lung cancer-like tissues in vitro with vascular cells and mesenchymal stem cells, which showed structures and immunohistological patterns that were similar to human lung cancer tissues. We named them “lung cancer … Show more

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Cited by 37 publications
(41 citation statements)
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“…Slides were counterstained with haematoxylin. This novel, highly sensitive method was used in our previous studies involving lung and nodal FFPE tissue, and lung cancer samples were used as a positive control …”
Section: Methodsmentioning
confidence: 99%
“…Slides were counterstained with haematoxylin. This novel, highly sensitive method was used in our previous studies involving lung and nodal FFPE tissue, and lung cancer samples were used as a positive control …”
Section: Methodsmentioning
confidence: 99%
“…TAM-derived IL6mediated STAT3 signaling pathway also found to increase the proliferation of human cancer stem cells (97). In different phases of lung cancer development and its therapeutic management, IL6 drives multiple molecular mechanisms responsible for the epithelial-mesenchymal transition (EMT) (98,99) and therapy resistance, such as infiltration of pro-tumor macrophages after irradiation through the upregulation of CCL2/CCL5 in vitro human and in vivo mouse lung tumor models (100). Therefore, the blockade of IL6 reprograms the TME to restrict lung cancer development and progression in experimental lung tumorigenesis models (101).…”
Section: Cytokines Il6mentioning
confidence: 99%
“…To overcome this challenge, we previously developed a novel method to generate CSC-like cells. We retrovirally transduced a set of defined factors, Octamer-binding transcription factor 3/4 (OCT3/4), Kruppel-like factor 4 (KLF4), and SRY-box transcription factor 2 (SOX2) into human colon [13] and lung [14] cancer cells, followed by culturing with a conventional serum-containing medium, but not a human embryonic stem cell medium. Cancer cells transduced with the three factors showed significantly enhanced CSC properties in terms of the expression of marker genes, formation of spheres, chemoresistance, and in vivo tumorigenicity, and were capable of forming tumors that were similar to human colon and lung cancer tissues in terms of both their structural and immunohistological patterns [13,14].…”
Section: Introductionmentioning
confidence: 99%
“…Moreover, we identified glycogen synthase kinase 3 beta (GSK3B) and interleukin-6 (IL-6) as novel potential therapeutic targets in colon and lung cancers, respectively, using organoids, 3D structures derived from the CSC-like cells [13,14]. However, this technique has not previously been applied in OS cells, and many aspects of the mechanism remain unexplained.…”
Section: Introductionmentioning
confidence: 99%