Interleukin-6 Deficiency Fails to Prevent Chronic Rejection After Aortic Allografts in Apolipoprotein E–Deficient Mice

Yacoub‐Youssef, Houda; Blaes, Nelly; Calise, Denis; Thiers, Jean-Claude; Therville, Nicole; Benoist, Hervé; Ségui, Bruno; Saati, Talal Al; Thomsen, Mögens

doi:10.1016/j.healun.2008.06.012

Cited by 6 publications

(4 citation statements)

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“…7 Chronic rejection models have been used widely to investigate the pathogenesis of chronic vascular rejection. [8][9][10][11][12][13] In conclusion, size discrepancy between donor and recipient aortas is not in obstacle in a mouse heterotopic aortic transplant if the sleeve technique is used. The approach we report in this study will help develop a better heterotopic aortic transplant model for basic and translational research on chronic rejection.…”

Section: Discussionmentioning

confidence: 81%

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2014

Exp Clin Transplant

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Objectives: We sought to develop a better approach in establishing a heterotopic aortic transplant model. Materials and Methods: We used "sleeve" operation and set up 2 heterotopic aortic transplant experimental groups (groups 1 and 2). In group 1, donors (BALB/c (H-2 d ) mice; weight, 25-30 g) had the same weight as the recipients (C57BL/6 (H-2 d ) mice; weight, 25-30 g); in group 2, donors had lower weights (BALB/c (H-2d) mice, weight, 15-20 g). Grafts were examined macroscopically and histologically 60 days after the transplant. Results: The thrombosis incidence was 10% in group 1 (5 of 50) and 2% in group 2 (1 of 50) (P < .05).Intestinal obstructions led to a high mortality rate in both groups: 8% in group 1 and 10% in group 2 (P < .05). Conclusions:The issue of size discrepancy between donor and recipient aortas is not a problem in a heterotopic aortic transplant model with the use of a sleeve technique, while the high incidence of intestinal obstructions must be considered.

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Section: Discussionmentioning

confidence: 81%

Untitled

2014

Exp Clin Transplant

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“…34 In relation to TA, elimination of recipient IL-6 slightly increases arteriosclerotic thickening in an aortic interposition model and systemic neutralization of this cytokine reduces arteriosclerotic thickening in a humanized model of vascular rejection. 35,36 Initially, after organ transplantation, the large majority of alloreactive T cells are activated toward allogeneic peptide-MHC presented by graft cells. 37 The mechanism by which IL-6 is immunostimulatory in our experiments is likely through its secretion by antigen presenting cells because we observe that IL-6 increases T cell proliferation and prevents T cell death in the spleen, which is a location where graft-derived antigen-presenting cells (but not vascular cells) affect T cell activation.…”

Section: Discussionmentioning

confidence: 99%

Graft-Derived IL-6 Amplifies Proliferation and Survival of Effector T Cells That Drive Alloimmune-Mediated Vascular Rejection

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Rey²,

Enns³

et al. 2016

Transplantation

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“…4 Furthermore, stimulation of macrophages with ApoE promotes their conversion from the proinflammatory M1 to the anti-inflammatory M2 phenotype 6 and suppresses the inflammatory response upon LPS stimulation. 7 Apolipoprotein E also inhibits platelet activation, aggregation, and regulates function of adaptive immunity including generation of cytotoxic T cells and production of immunoglobulin G. [8][9][10] Apolipoprotein E is composed of 2 distinct functional domains. The N-terminal domain that contains a receptorbinding region located between residues 133 and 149 and the C-terminal domain that includes a lipid-binding region.…”

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confidence: 99%