Interleukin-6 expression by osteoblast-like MG63 cells challenged with four acrylic bone cements

Cenni, E.; Granchi, Donatella; Ciapetti, G.; Stea, Susanna; Savarino, L.; Corradini, Alessandra

doi:10.1163/156856201750180951

Cited by 7 publications

(4 citation statements)

References 17 publications

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“…In addition to this, the fact that MG63 cells display low levels of RANKL expression, strongly suggests that intracellular mechanisms other than those activated by RANKL/RANK interaction might be involved in the osteoclastogenic response induced by conditioned media from MG63 cell cultures. In that context, it is important to note that tumour cells have the ability to produce many osteoclastogenic factors, in addition to M-CSF and RANKL, namely, parathyroid hormone-related peptide, IL-1, IL-6, IL-11, TNF-a and VEGF [51][52][53]. The characterization of the composition of MG63 conditioned medium in respect to relevant molecules is underway.…”

Section: Discussionmentioning

confidence: 99%

Paracrine-mediated osteoclastogenesis by the osteosarcoma MG63 cell line: is RANKL/RANK signalling really important?

Costa‐Rodrigues

Teixeira²,

Fernandes

2011

Clin Exp Metastasis

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Although in the past little attention has been paid to the influence of osteosarcoma cells in osteoclast function, recent studies suggest a close relationship between osteosarcoma aggressiveness and osteoclastic activity. The present study addresses the paracrine effects of MG63 cells, a human osteosarcoma-derived cell line, on the differentiation of peripheral blood osteoclast precursor cells (PBMC). PBMC were cultured for 21 days in the presence of conditioned media from MG63 cell cultures (CM) collected at 48 h (CM_MG1), 7 days (CM_MG2) and 14 days (CM_MG3). MG63 cell cultures displayed the expression of ALP and BMP-2 and, also, the osteoclastogenic genes M-CSF and RANKL, although with a low expression of RANKL. PBMC cultures supplemented with CM presented an evident osteoclastogenic behavior, which was dependent on the culture period of the MG63 cells. The inductive effect appeared to be more relevant for the differentiation and activation genes, c-myc and c-src, and lower for genes associated with osteoclast function. In addition, PBMC cultures displayed increased functional parameters, including calcium phosphate resorbing activity. Assessment of the PBMC cultures in the presence of U0126, PDTC, and indomethacin suggested that in addition to MEK and NFkB pathways, other signaling mechanisms, probably not involving RANKL/RANK interaction, might be activated in the presence of conditioned medium from MG63. In conclusion, MG63 cell line appears to induce a significant paracrine-mediated osteoclastogenic response. Understanding the mechanisms underlying the interaction of osteosarcoma cells and osteoclasts may contribute to the development of new potential approaches in the treatment of such bone metabolic diseases.

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Section: Discussionmentioning

confidence: 99%

Paracrine-mediated osteoclastogenesis by the osteosarcoma MG63 cell line: is RANKL/RANK signalling really important?

Costa‐Rodrigues

Teixeira²,

Fernandes

2011

Clin Exp Metastasis

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“…However, long‐term stability and material‐mediated cytotoxicity pose serious implications for usage. For example, osteolysis has been attributed to wear debris from a variety of orthopedic materials 51–56, 67–70. There are many in vitro studies found in the literature that suggest that UHMWPE is oxidatively unstable in certain environments 48, 58, 59, 71–76.…”

Section: Resultsmentioning

confidence: 99%

“…Despite ∼1.4 million total joint replacements performed annually,49 a shortcoming of UHMWPE is limited implant longevity due to wear of the acetabular liners 50. Wear debris has been directly linked to osteolysis,50–55 which is triggered by ˙ OH inflammation 56. A graver dilemma associated with UHMWPE is gamma (γ)‐sterilization, which is typically performed on medical devices that cannot be sanitized by boiling 57.…”

Section: Introductionmentioning

confidence: 99%

Thermally responsive poly[N‐isopropylacrylamide‐co‐2‐hydroxyethylacrylate] colloidal crystals included in β‐cyclodextrin for controlled drug delivery

Mathews¹,

Cho²,

Kim³

et al. 2009

J of Applied Polymer Sci

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Novel temperature-sensitive poly [N-isopropylacrylamide-co-2-hydroxyethylacrylate] [P(NIPAAmHEAc)] colloidal crystals containing b-cyclodextrin (b-CD) were prepared. These reversibly tunable supramolecular inclusion complexes display brilliant colors, due to the self-assembly of several structured nanoparticles to form crystal structures. The hydrogels displayed rainbow colors upon the variation of the b-CD concentration and can be made either visible or invisible by simply changing the temperature. The inclusion complexes were characterized using NMR spectroscopy, laser light scattering, UV-visible spectroscopy, and viscometer measurements. Compared with the conventional poly(N-isopropylacrylamide) gel, the b-CD-incorporated hydrogels in aqueous solution showed a lower critical solution temperature of about 57 C, which can be attributed to the hydrogen bonding between P(NIPAAm-HEAc) included in b-CD and water, which is enhanced by the hydrophilic groups of b-CD. The use of these materials as drug carriers was also evaluated by making use of their thermo-responsive behavior.The release time of a model drug, 5-fluorouracil (5-FU), from the novel inclusion complexes was prolonged in comparison to that from P(NIPAAm-HEAc) without b-CD. The significance of the present drug delivery system is the mixing of the gel and drug without any chemical reaction at room temperature, resulting in a gelled drug depot for slow and controlled release.

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“…For example, osteolysis has been attributed to wear debris from a variety of orthopedic materials. [51][52][53][54][55][56][67][68][69][70] There are many in vitro studies found in the literature that suggest that UHMWPE is oxidatively unstable in certain environments. 48,58,59,[71][72][73][74][75][76] These studies offer confirming experimental evidence, specifying that oxidation of UHMWPE units can occur because of residual radicals present within the material after ␥ sterilization.…”

Section: Radical Chemistry Of Uhmwpementioning

confidence: 99%

Electron paramagnetic resonance analyses of surface radical chemistries of γ‐sterilized orthopedic materials: Implications pointing to cytotoxicity via wear debris–induced inflammation

et al. 2005

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In this research, electron paramagnetic resonance (EPR) spin-trapping was utilized to determine if surface radical chemistries occur for gamma (gamma)-sterilized orthopedic materials-ultra-high molecular weight polyethylene (UHMWPE) and the novel, hybrid, diurethane dimethacrylate (DUDMA)-based RHAKOSS. The materials' ability to competitively chelate catalytic ferrous ions (Fe(2+)) or readily reduce ferric ions (Fe(3+)), and hydrogen peroxide (H(2)O(2)) directly, in facilitating the Fenton reaction (FR), is indicative of cytotoxicity. Validations with a radical scavenger aids to confirm a radical mechanism. In conjunction, materials were thermally annealed and characterized by attenuated total reflectance-Fourier-transform infrared (ATR-FTIR) spectroscopy in order to explore accelerated oxidative degradation induced by residual radicals evolving from gamma-sterilization. Particularly, there was a significant decrease in spin-adduct peak areas obtained from the reduction of H(2)O(2) in the presence of RHAKOSS or UHMWPE, evaluated against their respective controls. Additionally, chelated Fe(2+) accelerated the rate of FR. This phenomenon suggests that the materials are not better chelators than the Fe-activating chelator, edta. Neither material had the propensity to readily reduce Fe(3+) to the relevant Fe(2+), as certified by a nonradical mechanism. Alternatively, the false spin-adduct signal acquired when chelated Fe(3+) is employed arises via the nucleophilic addition of water onto the DMPO spin trap. Residual radicals in UHMWPE did not recombine/terminate following thermal annealing in an inert atmosphere. The radicals in RHAKOSS, however, did recombine under mild heating in an oxidizing or inert atmosphere. Both materials displayed quenching of ( )OH; however, for UHMWPE, this mechanism was jointly accountable for its accelerated degradation, evidenced by ATR-FTIR. Quenching of ( )OH by the silica found in RHAKOSS manifested in a competing effect that counterbalanced the observed FR. Implanted RHAKOSS is not likely to promote cytotoxicity and should not degrade, but the damaging effect of gamma sterilization on UHMWPE is a serious dilemma confronting its long-term durability and biocompatibility.

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Interleukin-6 expression by osteoblast-like MG63 cells challenged with four acrylic bone cements

Cited by 7 publications

References 17 publications

Paracrine-mediated osteoclastogenesis by the osteosarcoma MG63 cell line: is RANKL/RANK signalling really important?

Paracrine-mediated osteoclastogenesis by the osteosarcoma MG63 cell line: is RANKL/RANK signalling really important?

Thermally responsive poly[N‐isopropylacrylamide‐co‐2‐hydroxyethylacrylate] colloidal crystals included in β‐cyclodextrin for controlled drug delivery

Electron paramagnetic resonance analyses of surface radical chemistries of γ‐sterilized orthopedic materials: Implications pointing to cytotoxicity via wear debris–induced inflammation

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