Interlobular Distribution of Hepatic Xenobiotic-Metabolizing Enzyme Activities in Cattle, Goats and Sheep1

Abstract

Microsomal and cytosolic enzymes that metabolize xenobiotics were measured in composite samples representing entire livers and in samples from three lobes, using livers of cattle, goats and sheep. Within individual species, concentrations of cytochrome P-450 and b5 and activities of NADPH cytochrome c reductase, aldrin epoxidase, aminopyrine N-demethylase, ethoxycoumarin O-deethylase, microsomal and cytosolic stilbene oxide (epoxide) hydrolase and glutathione S-transferase were not different (P greater than .05) among the various hepatic lobes. Among species, several activities differed (P less than .05), with cattle livers generally having lower values than sheep or goats.

In vitro cytochrome P450‐mediated hepatic activities for five substrates in specific pathogen free chickens

“…Compared with other animal species, the chicken total CYP content was close to those of some other species, including humans, cats (Gupta & Abou‐Donia, 1998), pigs (Monshouwer et al ., 1996), snakes, frogs and trout fish (Schwen & Mannering, 1982). However, it was less than half of the values of some other species such as guinea‐pig, hamster, mouse, rabbit, rat (Gupta & Abou‐Donia, 1998), monkey (Shimada et al ., 1997), horse (Lakrutz et al ., 2000), ruminants (Wisniewski et al ., 1987), American alligator (Mayeaux & Winston, 1998) and beagle dogs in this study. From Table 1, we did not find a good correlation between the type of feed (carnivorous or herbivorous) or the basal metabolic rate (the smaller the species, the higher its basal metabolic rate) and the total CYP contents.…”

Effect of tulathromycin on abomasal emptying rate in healthy lactating goats

“…The elimination half time of acetaminophen in this study appeared to be shorter than that reported for cattle (Grochowina and Janus, 2007;Ehsani-Kheradgerdi et al, 2011) and lambs (Sharifi et al, 2009). This result in consistent with a more active metabolism in goats than in cattle or sheep, with goats having a significantly higher activity of UDP-glucuronyltransferase (Short et al, 1988) and higher liver concentrations of cytochrome p-450, NADPH cytochrome c reductase, aldrin epoxidase, aminopyrine Ndemethylase, ethoxycoumarin O-deethylase, microsomal and cytosolic stilbene oxide (epoxide) hydrolase and glutathione s-transferase than sheep and cattle (Wisniewski et al, 1987). Acetaminophen is metabolized primarily by conjugation with sulfate and glucuronide and in goats the glucuronide metabolite is the predominant moiety in plasma (Ali et al, 1996).…”

Pharmacokinetic behavior of marbofloxacin after intravenous and intramuscular administrations in adult goats

“…Moreover, only a few studies relating to the pharmacokinetics of fluoroquinolones have been performed in goats and, although lots of studies about quinolones have been performed in ruminants, differences in the pharmacokinetic behaviour of some drugs between goats and other ruminant species, like sheep and cattle, have been described (Dalvi et al. , 1987; Wisniewsky et al. , 1987; Short et al.…”