Intermediary carbohydrate metabolism in embryonic life

Needham, Joseph; Nowinski, Wiktor W.

doi:10.1042/bj0311165

Cited by 57 publications

(10 citation statements)

References 20 publications

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“…Glyceraldehyde, even in much higher concentrations, did not appreciably affect the respiration of normal liver, kidney and brain. The inhibition of glycolysis by glyceraldehyde was demonstrated by Ashford [4] and Holmes [5] in brain tissue and by Needham and Nowinski [6] in chick embryos.…”

Section: Introductionmentioning

confidence: 97%

Anticancer Effect of dl-Glyceraldehyde and 2-Deoxyglucose in Ehrlich Ascites Carcinoma Bearing Mice and Their Effect on Liver, Kidney and Haematological Parameters

et al. 2013

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Cancer cells generally exhibit increased glycolysis for ATP generation (the Warburg effect). Compounds that inhibit glycolysis have potential applications in cancer treatment. dl-glyceraldehyde (DLG) and 2-Deoxyglucose (2-DG) have been proven effective in the inhibition of glucose metabolism. Ehrlich ascites carcinoma (EAC) cells were injected intraperitoneally (i.p) in 10-12 weeks old Swiss albino mice, weighing between 20 and 30 g. The anticancer activity of DLG and 2-DG were determined by tumor volume, tumor weight, viable and nonviable tumor cell count, average survival time, percentage increase in life span and tumor inhibition ratio. The blood samples were obtained for biochemical analysis after 9 days of treatment to study the effect on liver, kidney and haematological parameters. Histopathological examination of liver and kidney was also performed. One-way ANOVA test and Dunnett's test were used for comparisons of parameters in study groups. Both DLG and 2-DG individually decreased the tumor weight, tumor volume, viable tumor cell count and significantly increased the life span of treated mice, however the combination was found to be better. The biochemical parameters of liver and kidney functions and haematological parameters were restored close to control group as compared with the EAC bearing mice. Histopathological examination of liver and kidney in EAC control group showed large areas of necrosis, congestion and mononuclear cell infiltration but such changes were not observed in liver and kidney sections observed after i.p injection of DLG and 2-DG for 9 days. Improvement was much better in the group where combination of these two drugs were used.

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Section: Introductionmentioning

confidence: 97%

Anticancer Effect of dl-Glyceraldehyde and 2-Deoxyglucose in Ehrlich Ascites Carcinoma Bearing Mice and Their Effect on Liver, Kidney and Haematological Parameters

et al. 2013

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“…The developing chick embryo is highly dependent on glucose oxidation to supply its energy needs (1) . It has also been shown that the fetal heart contains the enzymes necessary for carrying out citric acid cycle oxidations (2)(3)(4) and oxidative phosphorylation (5) .…”

Section: Introductionmentioning

confidence: 99%

Changes in Glucose Oxidation During Growth of Embryonic Heart Cells in Culture

Warshaw

Rosenthal

1972

The Journal of Cell Biology

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A rapid and convenient method has been utilized to investigate glucose oxidation during growth of chick embryo heart cells in tissue culture. Primary isolates of chick embryo heart cells showed exponential growth when plated at low densities and exhibited density-inhibited growth as cultures became confluent. The density-dependent growth inhibition of chick embryo heart cells is associated with a marked decrease in the specific activity of glucose oxidation to CO2. This decrease in glucose oxidation was observed as density increased as either a function of time in culture or as related to initial plating density. The decrease in 14CO2 production associated with density-dependent inhibition of growth is due to a marked decrease in activity of the pentose phosphate pathway.

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“…An investigation of the effect of adrenaline on embryonic muscle glycogen was of interest because of the findings of Needham & Nowinski [1937] that embryonic chick tissue was unable to glycolyse glycogen, being deficient, or partly so, in four of the essential enzyme components of phosphorylating glycolysis.…”

Section: Resultsmentioning

confidence: 99%

The effect of adrenaline on embryonic chick glycogen in vitro as compared with its effect in vivo

Gill

1938

Biochemical Journal

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MUCH less appears to be known of the mode of action of adrenaline on the breakdown of liver glycogen than of its mode of action on muscle glycogen. In muscle it has been shown that adrenaline initiates a breakdown of glycogen to lactic acid not only in an isolated perfused limb but in pieces of excised muscle in vitro [Hegnauer & Cori, 1934]. Lately Nachmansohn et al. [1936; 1937] have shown that adrenaline will act even on a muscle " brei ", increasing the rate of formation of lactic acid and causing an accumulation of hexosemonophosphate, which seems to indicate that adrenaline may be acting here as a specific accelerator of one of the enzymes taking part in the phosphorylated glycogen glycolysis system in muscle.

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Intermediary carbohydrate metabolism in embryonic life

Cited by 57 publications

References 20 publications

Anticancer Effect of dl-Glyceraldehyde and 2-Deoxyglucose in Ehrlich Ascites Carcinoma Bearing Mice and Their Effect on Liver, Kidney and Haematological Parameters

Anticancer Effect of dl-Glyceraldehyde and 2-Deoxyglucose in Ehrlich Ascites Carcinoma Bearing Mice and Their Effect on Liver, Kidney and Haematological Parameters

Changes in Glucose Oxidation During Growth of Embryonic Heart Cells in Culture

The effect of adrenaline on embryonic chick glycogen in vitro as compared with its effect in vivo

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