Intermediate Filaments: A Chemically Heterogeneous, Developmentally Regulated Class of Proteins

Lazarides, Elias

doi:10.1146/annurev.bi.51.070182.001251

Cited by 982 publications

(472 citation statements)

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“…A key organizational difference between cytokeratins and other intermediate filaments is the interaction of the keratins (tonofilaments) with desmosomes and related structures (5,6,11,(23)(24)(25)(26). Our results show that cytokeratin organization in HeLa cells depends on interactions with cell membrane structures which provide a peripheral network of stabilizing attachment sites for keratin filaments, as well as with microtubules and microfilaments, which may provide a cytoplasmic framework .…”

Section: Discussionmentioning

confidence: 71%

“…Unlike microtubules and microfilaments, whose distributions are universal in vertebrate cells and whose functions are well characterized (1)(2)(3)(4), intermediate filaments are generally restricted in distribution to specific cell types, and their functions remain unresolved (5,6). Lazarides (5) has suggested that they participate in the organization and mechanical integration of the cytoplasm .…”

mentioning

confidence: 99%

“…However, no biochemical inhibitors analogous to cytostatic drugs used to destabilize microtubules or microfilaments have been found that specifically interfere with cytokeratin structure or 1788 LOREN W. KNAPP, W. MICHAEL O'GUIN, and ROGER H . SAWYER Department of Biology, University of South Carolina, Columbia, South Carolina 29208 function (6) . Recent immunofluorescence studies of cytokeratins during mitosis in certain epithelial cell types, including HeLa cells, have demonstrated that the otherwise immutable organization of cytokeratins changes from an extended filamentous arrangement to a condensed, spheroidal form as the cells divide, with the re-establishment of extended filaments following division (15,16).…”

mentioning

confidence: 99%

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Rearrangement of the keratin cytoskeleton after combined treatment with microtubule and microfilament inhibitors.

Knapp¹,

O’Guin²,

Sawyer³

1983

The Journal of Cell Biology

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In addition to containing microtubule and microfilament systems, vertebrate epithelial cells contain an elaborate keratin intermediate-filament cytoskeleton . Little is known about its structural organization or function . Using indirect immunofluorescence microscopy with an antikeratin antiserum probe, we found that destabilization of microtubules and microfilaments with cytostatic drugs induces significant alterations in the cytoskeletal organization of keratin filaments in HeLa and fetal mouse epidermal cells . Keratin filament organization was observed to undergo a rapid (1-2 h) transition from a uniform distribution to an open lattice of keratin fibers stabilized by membrane-associated focal centers . Since addition of any one drug alone did not elicit significant organizational change in the keratin cytoskeleton, we suggest that microfilaments and microtubules have a combined role in maintaining the arrangement of keratin in these cells . Vimentin filaments, the only other intermediatesized filaments found in HeLa cells, did not co-distribute with keratin in untreated or drugtreated cells . These findings offer a new way to approach the study of the dynamics and functional roles of the keratin cytoskeleton in epithelial cells .

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Section: Discussionmentioning

confidence: 71%

mentioning

confidence: 99%

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confidence: 99%

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Rearrangement of the keratin cytoskeleton after combined treatment with microtubule and microfilament inhibitors.

Knapp¹,

O’Guin²,

Sawyer³

1983

The Journal of Cell Biology

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“…l(A)] are characterized immunocytochemically by the most intense staining for vimentin. The intermediate filament is found in the majority of mesenchymal cells as well as in nearly all cell types grown in tissue culture studies (Lazarides, 1982). In fact, there is some vimentin staining in all cells of regions 1-5.…”

Section: Discussionmentioning

confidence: 98%

“…In fact, there is some vimentin staining in all cells of regions 1-5. Similar to the other intermediate filament types, a structural function is also assumed for vimentin (Lazarides, 1982).…”

Section: Discussionmentioning

confidence: 99%

Different cell populations in bovine trabecular meshwork: An ultrastructural and immunocytochemical study

Flügel

Tamm

Lütjen–Drecoll

1991

Experimental Eye Research

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Bovine outflow tissue differs markedly from that of humans. Tissue culture studies on the cells of this region are often compared with those of primate trabecular meshwork cells. A thorough cytological and immunocytochemical characterization of the cells of the bovine chamber angle is lacking. We have therefore investigated the cells of the pectinate ligament, the reticular meshwork, the region adjacent to the aqueous plexus, the connective tissue region between reticular meshwork and ciliary muscle and the ciliary muscle itself, ultrastructurally and immunocytochemically with staining for the cytoskeletal proteins vimentm and desmin, for a-smooth muscle-actin and rough endoplasmic reticulum (rER).In the pectinate ligament and in the region adjacent to the aqueous plexus, the cells were found to have especially abundant rER and glycogen in their cytoplasm. Vimentin was abundant in the reticular meshwork as positive staining was seen both in frozen and partin sections. Alpha-smooth muscle-a&in could be found in the region connecting ciliary muscle and reticular meshwork as well as in a small area adjacent to the posterior capillary loops of the aqueous plexus. Ultrastructurally, these cells resembled myofibroblasts. The ciliary muscle cells stained both for vimentin and for a-smooth muscle actin.

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Antibodies to Intermediate Filament Proteins

Miettinen¹,

Lehto²,

Virtanen³

1985

Arch Dermatol

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Intermediate Filaments: A Chemically Heterogeneous, Developmentally Regulated Class of Proteins

Cited by 982 publications

References 0 publications

Rearrangement of the keratin cytoskeleton after combined treatment with microtubule and microfilament inhibitors.

Rearrangement of the keratin cytoskeleton after combined treatment with microtubule and microfilament inhibitors.

Different cell populations in bovine trabecular meshwork: An ultrastructural and immunocytochemical study

Antibodies to Intermediate Filament Proteins

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