Internal bremsstrahlung spectral shapes of<sup>45</sup>Ca and<sup>3a</sup>S

Babu, Ram; Murty, K. N.; Murty, V A Narasimha

doi:10.1088/0305-4616/2/5/009

Cited by 8 publications

(1 citation statement)

References 13 publications

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“…Although there is a sufficient difference between the β energies emitted by 3 H (E av = 5.7 keV, E max = 18.6 keV) and 45 Ca (E av = 77 keV, E max = 252 keV), the internal bremsstrahlung effect of the 45 Ca spectra (Babu et al, 1976) emitted in the β-decay may lead to sufficient tailing of the peak. This may lead to fictitious peak area measurement in the 3 H window and hence lead to inaccurate results, especially for higher activity concentrations of 45 Ca compared to 3 H. So for better quantification of radionuclides in the dual-label mode, it is crucial to verify the effect of 45 Ca in the 3 H regions, especially with increasing quench.…”

Section: Discussionmentioning

confidence: 99%

Liquid scintillation based quantitative measurement of dual radioisotopes (3H and 45Ca) in biological samples for bone remodeling studies

Song

Sharma

Bhattacharyya

2012

Applied Radiation and Isotopes

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Acute and prolonged bone complications associated with radiation and chemotherapy in cancer survivors underscore the importance of establishing a laboratory-based complementary dual-isotope tool to evaluate short- as well as long-term bone remodeling in an in vivo model. To address this need, a liquid scintillation dual-label method was investigated using different scintillation cocktails for quantitative measurement of 3H-tetracycline (3H-TC) and 45Ca as markers of bone turnover in mice. Individual samples were prepared over a wide range of known 45Ca/3H activity ratios. Results showed that 45Ca/3H activity ratios determined experimentally by the dual-label method were comparable to the known activity ratios (percentage difference ~2%), but large variations were found in samples with 45Ca/3H activity ratios in range of 2–10 (percentage difference ~ 20–30%). Urine and fecal samples from mice administered with both 3H-TC and 45Ca were analyzed with the dual-label method. Positive correlations between 3H and 45Ca in urine (R = 0.93) and feces (R = 0.83) indicate that 3H-TC and 45Ca can be interchangeably used to monitor longitudinal in vivo skeletal remodeling.

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Section: Discussionmentioning

confidence: 99%