Internal lipids of felted, yellowed and pathologically thin wool

Tkachuk, V. M.; Havrylyak, V. V.; Stapay, P. V.; Sedilo, H. M.

doi:10.15407/ubj86.01.131

Cited by 4 publications

(3 citation statements)

References 17 publications

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“…The amount of sweat was determined by extracting its salts with water, and the concentration of hydrogen ions (pH) in the extract was determined using a universal EV-74 ionometer [23].…”

Section: Methodsmentioning

confidence: 99%

“…Special attention is paid to studies of 18-methyleicosanoic acid, which is part of the hydrophobic lipid surface of hair and covalently binds structural lipids to proteins [18]. As shown, internal lipids have a decisive influence on the formation of the surface properties of hair and its ability to protect against external negative factors [19]. They have the ability to change the structure of the fiber, reducing the moisture content and permeability, and increasing its tensile strength [20].…”

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confidence: 99%

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Internal lipids and their fatty acids composition in a sheep wool fiber under biodestruction with fleece microorganisms

Tkachuk,

Stapay,

Ohorodnyk

et al. 2024

Ukr.Biochem.J.

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Microbiological destruction of fibers is a common damage to sheep’s wool. Considering the defining role of internal lipids in the formation of wool fibers surface the aim of the work was to study the structure and lipid composition of the normal and damaged wool. The research was carried out on ewes of the Askanian fine-wool breed. The content of microorganisms was estimated after sowing on dense nutrient environments. Wool fibers surface was studied by scanning electron microscopy, the content of internal lipids by thin layer chromatography after preliminary alkaline hydrolysis of the fiber, and fatty acids composition by gas-liquid chromatography. Biodestructed wool was shown to contain almost three times more bacteria, as well as higher levels of actinomycetes and mushrooms compared to intact wool. The violation of the cuticular layer was detected as the result of the fleece microflora activity. In a defective wool the content of the free internal lipids and non-esterified fatty acids was increased while the content of protein-bound lipids and esterified cholesterol as well as of ceramides was decreased as compared to normal wool. The level of 18-methyleicosanoic acid in the protein-bound lipids of damaged wool was decreased, indicating the destruction of the thioester bonds by which structural lipids are covalently linked to proteins through 18-methyleicosanoic acid. Keywords: 18-methyleicosanoic acid, biodestruction, fatty acids, internal lipids, microorganisms, protein-bound lipids, sheep’s wool fiber

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“…The amount of sweat was determined by extracting its salts with water, and the concentration of hydrogen ions (pH) in the extract was determined using a universal EV-74 ionometer [23].…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Internal lipids and their fatty acids composition in a sheep wool fiber under biodestruction with fleece microorganisms

Tkachuk,

Stapay,

Ohorodnyk

et al. 2024

Ukr.Biochem.J.

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“…Суттєвих змін у пожовтілій вовні зазнають внутрішні ліпіди [3]. З огляду на це, метою нашої роботи було встановити вплив процесів пожовтіння на кількісний та якісний склад внутрішніх ліпідів вовняного волокна.…”

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Вплив Процесів Пожовтіння На Кількісний Та Якісний Склад Внутрішніх Ліпідів Вовни Овець

Ткачук,

Огородник

2023

Grundlagen Der Modernen Wissenschaftlichen Forschung

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Ткачук Віталій Мирославович доктор сільськогосподарських наук, в.о. доцента кафедри тваринництва та кормовиробництва Львівський національний університет природокористування ORCID ID: 0000-0002-7428-9973 Огородник Наталія Зіновіївна доктор ветеринарних наук, професор завідувачка кафедри тваринництва та кормовиробництва Львівський національний університет природокористування УКРАЇНА Анотація. Представлені результати вивчення впливу процесів пожовтіння вовни на кількісний та якісний склад внутрішніх ліпідів. Показано, що пожовтіла вовна характеризується нижчим вмістом вільних внутрішніх ліпідів (на 18%), яке відбувається за рахунок зменшення фракції етерифікованого холестеролу (на 21 %) та церамідів (на 5 %). Пожовтіння вовни супроводжується процесами гідролізу її ліпідних компонентів, на що вказує зростання кількості неетерифікованих жирних кислот та зменшення етерифікованого холестеролу.

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The ratio of structural proteins in the wool of sheep of different breeds and with different categories of fibers depending on the zones of their breeding

Stapay,

Buslyk,

Mykhaliuk

et al. 2024

Bìol. Tvarin

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Wool fiber consists of a complex of protein-keratins, which differ in both their physical and chemical properties and functional characteristics. Despite the progress achieved in the study of the structural organization of fibers, the issue of the influence of environmental factors, the nature and level of nutrition, as well as breed characteristics, remains poorly understood. With this in mind, we investigated the ratio of structural proteins in the wool of sheep of different breeds, which are bred in separate zones of Ukraine, in particular, fine-wool, semi-fine-wool, and coarse-wool. Therefore, during the study of the structural proteins of the wool of sheep of different breeds, which differ among themselves in the thinness of the fibers, it was established that by the method of electrophoretic separation, these proteins are clearly divided into three different groups, in particular, these are proteins of intermediate filaments (IF) of I and II types with a molecular weight of 55–65 and 45–55 kDa, respectively, as well as keratin-associated proteins (KAP) with a molecular weight of 25–35 kDa. However, in some breeds, in particular, the Askanian meat-wool of the black-headed type and the Ukrainian Mountain Carpathian type, two more fractions of proteins were found among IF type I, which almost do not differ from each other in terms of percentage. It was established that within the studied groups of sheep, certain interbreed features are observed, so in the group of thin-wooled breeds, the largest percentage falls on IF II (35.9%). The other two factions account for almost the same percentage — 32.1% (IF I) and 31.8% (KAP). An almost similar picture of the ratio of protein fractions was recorded in the group of animals with coarse wool. At the same time, in the group of sheep with semi-fine wool, in particular, the fibers of sheep of the Ascanian meat-wool breed have the highest percentage of KAP and, accordingly, less IF II, and in the fibers of the Ascanian meat-wool black-headed type, respectively, less proteins of IF II and KAP. Therefore, the obtained data clearly indicate breed characteristics of the content and ratios of certain groups of proteins, which can be genetically determined, and therefore serve as appropriate markers both within individual breeds and within breeds with different fiber categories.

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Internal lipids of felted, yellowed and pathologically thin wool

Cited by 4 publications

References 17 publications

Internal lipids and their fatty acids composition in a sheep wool fiber under biodestruction with fleece microorganisms

Internal lipids and their fatty acids composition in a sheep wool fiber under biodestruction with fleece microorganisms

Вплив Процесів Пожовтіння На Кількісний Та Якісний Склад Внутрішніх Ліпідів Вовни Овець

The ratio of structural proteins in the wool of sheep of different breeds and with different categories of fibers depending on the zones of their breeding

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