“…Based on the findings of previous reports, we administered 2-20 mCi of I-131-labelled Suicide-gene therapy for HCC Y Kawashita et al lipiodol to each animal in a total volume of 100 ml, diluted with cold lipiodol 24 h after transfection. 41 Subsequently, 48 and 72 h after transfection, the tumors, livers and lungs of the animals were excised and immersed in 1200 ml of 3 Â reporter lysis buffer, and then were cut with sterile scissors. The mixture was transferred to a 15 ml falcon tube and homogenized using a sonicator for 5-10 min, followed by centrifugation at 3000 r.p.m.…”