Attention to Same-Sex Intimate Partner Violence (SSIPV) has increased in the last decades. Isolating behaviors, however, have been poorly studied, despite the increased vulnerability to isolation experienced by gay and lesbian couples. The aim of the present study was to examine the independent effects of minority stressors (experiences of discrimination, perceived stigma, and internalized homonegativity), emotion dysregulation, and adult attachment dimensions (attachment avoidance and anxiety) on isolating behaviors perpetration. One hundred and forty-eight gay or lesbian individuals (Mage = 32.48; SD = 10.91) involved in a same-sex relationship participated in the study and completed a self-report online questionnaire. A multiple regression model was used to examine the relationship between the variables in the study. Only attachment anxiety was associated with isolating behaviors. In contrast, attachment avoidance, experiences of discrimination, perceived stigma, internalized homonegativity, and emotion dysregulation did not influence perpetration of isolating behaviors. The present study highlights the influence of attachment anxiety on isolating behaviors, suggesting the role of dysfunctional mechanisms of interpersonal distance regulation. The obtained results provide information at the theoretical and clinical level.