International Clones of Klebsiella pneumoniae and Escherichia coli with Extended-Spectrum β-Lactamases in a Czech Hospital

Abstract

A 2-month survey of extended-spectrum ␤-lactamase (ESBL) producers was performed in a Czech hospital. Klebsiella pneumoniae produced SHV-2, -5, or -12, Escherichia coli produced CTX-M-9 or -15, and other species produced TEM-92 or -132. All K. pneumoniae and E. coli isolates belonged to sequence types (STs) or clonal complexes (CCs) spread across the world (K. pneumoniae clonal complex 11 [CC11], CC14, and sequence type 101 [ST101] and E. coli CC31, CC73, CC131, and CC405) and carried various plasmids (mainly with A/C-and FII-type replicons).

Plasmid-Mediated Resistance to Cephalosporins and Fluoroquinolones in Various Escherichia coli Sequence Types Isolated from Rooks Wintering in Europe

“…The sampling site in the Czech Republic was located in the National Nature Reserve, which is surrounded by fields, agricultural production areas, and urban agglomerations and which has a wastewater treatment plant and garbage dumps nearby. The distribution of ESBL genes closely corresponded to that previously described in hospital facilities (29,30), domestic animals (31), and the environment, including wild birds and wastewater treatment plant effluent (5,32,33), within that country. In Germany, the highest distribution of ESBL-producing E. coli strains was found at two sampling sites.…”

A Swordless Knight: Epidemiology and Molecular Characteristics of the bla KPC -Negative Sequence Type 258 Klebsiella pneumoniae Clone

“…Phylogenetically, the ST-258 clone is not related to known virulent K. pneumoniae clones associated with liver abscesses or pneumonia, such as the ST-23 and the ST-82 clonal complexes (CC) (7,46). It is, however, closely related to a single-locus variant strain, ST-11, that has been reported as a common hospital-associated clone from several European countries (15,24,39). Further genomic studies are required in order to explore whether ST-258 has indeed evolved from ST-11.…”

Identification and characterization of CTX-M-15 producing Klebsiella pneumoniae clone ST101 in a Hungarian university teaching hospital

“…This sequence type was associated with various β-lactam resistance mechanisms in different parts of the world: CTX-M-15 production in France, Greece and Italy; SHV-12 production in the Czech Republic; OXA-48 production in Spain, Libya, France, Tunisia, Morocco, Switzerland and South Africa; KPC-2 production in Italy and Spain [32,[35][36][37][38][39][40][41][42]. In our institution the presence of CTX-M-15 producing ST101 seemed to be restricted both timely and spatially, as the majority of the isolates originated from a single department with a decreasing incidence ( Tables II and III).…”