Acid Mine Drainage (AMD) has become an important issue due to its significant ecological pollution. In this paper, phytoremediation technology and mechanism for AMD were investigated by hydroponic experiments, using six wetland plants (Phragmites australis, Typha orientalis, Cyperus glomeratus, Scirpus validus, Iris wilsonii, Juncus effusus) as research objects. The results showed that (1) the removal of sulfate from AMD was highest for Juncus effusus (66.78%) and Iris wilsonii (40.74%) and the removal of Mn from AMD was highest for Typha orientalis (>99%) and Phragmites australis (>99%). In addition, considering the growth condition of the plants, Juncus effusus, Iris wilsonii, and Phragmites australis were finally selected as the dominant plants for the treatment of AMD. (2) The removal pathway of pollutants in AMD included two aspects: one part was absorbed by plants, and the other part was removed through hydrolysis and precipitation processes. Our findings provide a theoretical reference for phytoremediation technology for AMD.