Exposures along the lower Kaministiquia River (near Thunder Bay, Ontario, Canada) provide insight into early Holocene lake level fluctuations and paleoenvironmental conditions in the northwestern Lake Superior basin. These exposures show at least two large paleochannels which were downcut into offshore sediments, and were later filled with [2 m of sand, *3 m of rhythmically laminated silt and clay, and *6 m of interbedded silt and sand. Buried by the rhythmically laminated silty clay unit is a wellpreserved organic deposit with abundant plant macrofossils from terrestrial and emergent taxa, including several upright tree trunks. Three AMS radiocarbon ages were obtained on wood and conifer cones from this deposit: 8,135 ± 25 (9,130-9,010 cal), 8,010 ± 25 (9,010-8,780 cal), and 7,990 ± 20 (8,990-8,770 cal) BP. This sequence records an early postglacial high-water phase, followed by the Houghton lowstand, and reflooding of the lower Kaministiquia River Valley. The drop in lake level associated with the Houghton phase forced the ancestral Kaministiquia River to downcut. By *9,100 cal (*8,100) BP, older channels eroded into subaqueous underflow fan deposits in the Thunder Bay area near Fort William Historical Park (FWHP) were abandoned and colonized by a Picea-Abies-Larix forest. Based on stratigraphic data corrected for differential isostatic rebound, the lake was below the Sault Ste. Marie bedrock sill between at least 9,100 cal (8,100) and 8,900 cal (8,000) BP. Shortly after 8,900 cal BP, the lake quickly rose and buried in situ lowland vegetation at FWHP with varved sediments. We argue that this transgression was due to overflow from glacial Lakes Agassiz or Ojibway associated with the retreat of the Laurentide Ice Sheet from the Nakina moraine and/or the Cochrane surge margins in the Hudson Bay Lowlands. A continued rise in lake level after 6,420 ± 20 (7,400 cal) BP at FWHP may record uplift of the North Bay outlet above the Sault Ste. Marie bedrock sill and the onset of the Nipissing transgression in the Lake Superior basin.