Interpretation of the thiobarbituric acid reactivity of rat liver and brain homogenates in the presence of ferric ion and ethylenediaminetetraacetic acid

Kikugawa, Kiyomi; Kojima, Takashi; Yamaki, Shinji; Kosugi, Hiroko

doi:10.1016/0003-2697(92)90102-d

Cited by 256 publications

(125 citation statements)

References 24 publications

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“…11 The degree of lipid peroxidation was determined in the myocardial tissues through biochemical assay of thiobarbituric acid-reactive substances (TBARS). 12 …”

Section: Antioxidant Enzyme Activities and Lipid Peroxidationmentioning

confidence: 99%

Overexpression of Glutathione Peroxidase Prevents Left Ventricular Remodeling and Failure After Myocardial Infarction in Mice

et al. 2004

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Background-Oxidative stress plays an important role in the pathophysiology of heart failure. We determined whether the overexpression of glutathione peroxidase (GSHPx) could attenuate left ventricular (LV) remodeling and failure after myocardial infarction (MI). Methods and Results-We created MI in 12-to 16-week-old, male GSHPx transgenic mice (TGϩMI) and nontransgenic wild-type littermates (WTϩMI) by ligating the left coronary artery. GSHPx activity was increased in the hearts of TG mice, with no significant changes in other antioxidant enzymes. LV concentrations of thiobarbituric acid-reactive substances measured in TGϩMI at 4 weeks were significantly lower than those in WTϩMI. The survival rate during 4 weeks of MI was significantly higher in TGϩMI than in WTϩMI, although the infarct size was comparable. LV cavity dilatation and dysfunction were significantly attenuated in TGϩMI. LV end-diastolic pressure was increased in WTϩMI and reduced in TGϩMI. Improvement of LV function in TGϩMI was accompanied by a decrease in myocyte hypertrophy, apoptosis, and interstitial fibrosis in the noninfarcted LV. Myocardial matrix metalloproteinase-9 zymographic and protein levels were increased in WTϩMI after 3 days but were attenuated in TGϩMI. Conclusions-Overexpression of GSHPx inhibited LV remodeling and failure after MI. Therapies designed to interfere with oxidative stress might be beneficial to prevent cardiac failure.

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“…11 The degree of lipid peroxidation was determined in the myocardial tissues through biochemical assay of thiobarbituric acid-reactive substances (TBARS). 12 …”

Section: Antioxidant Enzyme Activities and Lipid Peroxidationmentioning

confidence: 99%

Overexpression of Glutathione Peroxidase Prevents Left Ventricular Remodeling and Failure After Myocardial Infarction in Mice

et al. 2004

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“…Serum MDA levels, referred to as thiobarbituric acid-reactive substance (TBARS), were measured according to the method described previously [11]. A volume sample of 250 µl of plasma was added to 35 µl of D/W and the color reaction was initiated by the addition of 25 µl of sodium dodecyl sulfate (SDS) (8.1%, W/V) and 190 µl of acetic acid (20%) in thiobarbituric acid (0.8%, W/V).…”

Section: Determination Of Malondialdehyde (Mda)mentioning

confidence: 99%

Remifentanil Attenuates Systemic Inflammatory Response in Patients undergoing Cardiac Surgery with Cardiopulmonary Bypass

Lee¹,

Tin²,

Kim³

et al. 2017

Arch Med.

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“…The TBARS concentration in the liver was measured using the colorimetric method (Kikugawa et al, 1992). The concentration of protein carbonyls was determined by the method of Evans et al (1999).…”

Section: Lqyroyhg Lq R[lgdwlyh Gdpdjh 7r\rnxql $Edohd Hw Domentioning

confidence: 99%

Vitamin E Modulates Radiation-induced Oxidative Damage in Mice Fed a High-Lipid Diet

Shin¹

2003

BMB Reports

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Interpretation of the thiobarbituric acid reactivity of rat liver and brain homogenates in the presence of ferric ion and ethylenediaminetetraacetic acid

Cited by 256 publications

References 24 publications

Overexpression of Glutathione Peroxidase Prevents Left Ventricular Remodeling and Failure After Myocardial Infarction in Mice

Overexpression of Glutathione Peroxidase Prevents Left Ventricular Remodeling and Failure After Myocardial Infarction in Mice

Remifentanil Attenuates Systemic Inflammatory Response in Patients undergoing Cardiac Surgery with Cardiopulmonary Bypass

Vitamin E Modulates Radiation-induced Oxidative Damage in Mice Fed a High-Lipid Diet

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