Interpretive optimisation strategy applied to the isocratic separation of phenols by reversed-phase liquid chromatography with acetonitrile–water and methanol–water mobile phases

Torres-Lapası́o, J.R.; Rosés, Martı́; Bosch, Elisabeth; García-Álvarez-Coque, M.C.

doi:10.1016/s0021-9673(00)00507-0

Cited by 49 publications

(10 citation statements)

References 26 publications

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“…The peak purity method (Eq. 14) has been used for this purpose [12,[15][16][17]21]. This method is based on the fraction of the peak that is overlapped by its neighbors, and it requires the knowledge of the total area of the peak (w i ) and the area of the peak overlapped by other peaks (w 0 i ).…”

Section: Peak Modeling and Optimization Algorithmmentioning

confidence: 99%

“…In this instance, we have relied in the semiempirical interpolation model we used previously to estimate peak width and asymmetry for the separation of some mixtures of neutral compounds [12]. It is based in a polynomically modified variant of the Gauss equation [12,[16][17][18] to simulate peak shape and takes into account peak height, width, and distortion. Together with the retention factor model has been used in this work to optimize the surfactant concentration and the pH of the buffer for separation of ten phenolic compounds with different acidities (pK a values in the range between 6.5 and 11) and different hydrophobicities (log P o/w values between 1 and 4).…”

Section: Introductionmentioning

confidence: 99%

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Optimization of the separation of ionizable compounds in micellar electrokinetic chromatography by simultaneous change of pH and SDS concentration

2007

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A method to optimize the separation in micellar EKC (MEKC) of mixtures of acidic compounds as a function of two parameters, pH and concentration of sodium dodecyl sulfate, has been developed. The method considers the prediction of the retention time and the shape of the peaks. The retention time is predicted from the retention factor model and the peak shape by a polynomically modified Gaussian function that considers peak width, asymmetry factor, and height. An algorithm to calculate the global resolution of the separation at any experimental pH and [SDS] has been applied. This algorithm provides a 3-D resolution map to easily detect the areas in which resolution for the separation of the compounds is maximum. Initial experiments to fit the models have been performed with a set of ten phenolic compounds with different hydrophobicities and pK(a) values, and therefore, expected to behave in a different way with changes of pH and surfactant concentration. The experiments encompassed a pH range from 6.7 to 11.1, and a sodium dodecyl sulfate concentration range from 40 to 80 mM. Through the proposed methodology, chromatograms have been simulated at different pH and [SDS] very accurately. Furthermore, the resolution at any experimental point within the studied ranges have been also calculated, giving an optimum resolution value at pH 6.7 and [SDS] = 72 mM.

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Section: Peak Modeling and Optimization Algorithmmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimization of the separation of ionizable compounds in micellar electrokinetic chromatography by simultaneous change of pH and SDS concentration

2007

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“…For all data weighted linear fitting with convenient transformation factors, (2.303 k) 2, was used to furnish homoscedastic error distributions [9]. The widely used empirical equation that relates the logarithm of the retention factor, k, with the volume fraction of organic modifier, by means of a polynomial relationship, was used here to predict retention.…”

Section: Assessment Of Resolutionmentioning

confidence: 99%

Towards the optimization of complementary systems in reversed-phase liquid chromatography

Vivó-Truyols

Torres-Lapası́o

Garcı́a-Alvarez-Coque

2002

Chromatographia

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Previously reported optimization methodology, which seeks complementary mobile phases (CMP) in isocratic chromatography, has been extended to include more than one system simultaneously (i.e. more than one organic solvent ancl/or column). In the literature the benefits of complementarity are not usually fully exploited -few working conditions giving rise to interactions as different as possible are examined, without developing a fully linked optimization. The proposed approach is compared criticallywith use of a single mobile phase orCMP which consider one system only. The strategy greatly expands the capability of isocratic chromatography in the analysis of complex samples that cannot be resolved by use of conventional methods.

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“…Nowadays, optimization methods have exploited most possibilities of isocratic elution and a variety of efficient approaches are available [1][2][3]. Reversed-phase liquid chromatography has become the primary mode to separate purine and pyrimidine derivatives [4][5][6], but it is not the only choice for this analytical task.…”

Section: Introductionmentioning

confidence: 99%

Separation of Tegafur and its Impurities by Reversed-Phase High-Perfomance Liquid Chromatography

Rotkaja¹,

Golushko²,

Kuprevics³

2014

Latvian Journal of Chemistry

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The chromatographic behavior of tegafur and its impurities on a naphthalene Cosmosil piNAP column under reversed-phase high-performance liquid chromatography conditions was examined. A good description of the retention was achieved through the application of statistical weights to the widely used quadratic relationships between the logarithm of the retention factor (log k) and the organic solvent concentration in the mobile phase. Optimum conditions for isocratic separation of the compounds were found with acetonitrile concentration of 10-30% in the mobile phase.

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Interpretive optimisation strategy applied to the isocratic separation of phenols by reversed-phase liquid chromatography with acetonitrile–water and methanol–water mobile phases

Cited by 49 publications

References 26 publications

Optimization of the separation of ionizable compounds in micellar electrokinetic chromatography by simultaneous change of pH and SDS concentration

Optimization of the separation of ionizable compounds in micellar electrokinetic chromatography by simultaneous change of pH and SDS concentration

Towards the optimization of complementary systems in reversed-phase liquid chromatography

Separation of Tegafur and its Impurities by Reversed-Phase High-Perfomance Liquid Chromatography

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