Interrelationships between the oocyte and somatic cells within the Graafian follicle of mammals

Baker, T. G.; Hunter, Richard

doi:10.1051/rnd:19780312

Cited by 24 publications

(7 citation statements)

References 15 publications

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“…The fact that higher levels of PRL were found in FF of intermediate OCCC than mature OCCC may reflect a higher vascular permeability to PRL in follicles yielding an intermediate OCCC, or alternatively, a process that increases levels in these follicles against the serum concentration. It has been suggested that PRL may stimulate the formation of an oocyte maturation inhibitor and that treatment of cultured rat follicles with an anti-PRL serum induced meiotic maturation (22). Our results strongly negate this possible effect of PRL on human oocytes, since high FF PRL concentrations were associated with successful fertilization and pregnancy, and may indicate that PRL might have a beneficial stimulatory role in the maturation process of the human oocyte.…”

Section: Discussioncontrasting

confidence: 68%

Gonadotropin and Prolactin Levels in Follicular Fluid of Human Ova Successfully Fertilized in Vitro

Laufer

Botero-Ruiz

DeCherney

et al. 1984

The Journal of Clinical Endocrinology & Metabolism

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Follicular fluid (FF) and oocytes were obtained from 94 follicles of 36 women for fertilization in vitro. Ovulation was induced with human menopausal gonadotropin, and follicular aspiration was performed 36 h after an ovulatory injection of hCG. The concentrations of immunoreactive hCG, FSH, and PRL were correlated with the degree of maturation of the oocyte-corona-cumulus complex mass (OCCC), fertilization, rate of cleavage, and the incidence of pregnancy after embryo transfer. Immature OCCC were derived from follicles that contained significantly lower levels of FSH than those from which intermediate and mature OCCC were derived (5.2 +/- 0.6 vs. 11.1 +/- 1.2 mlU/ml; P less than 0.05). FF from oocytes that were successfully fertilized contained higher levels of both hCG and FSH than FF surrounding oocytes that did not fertilize (136.7 +/- 8.7 vs. 108.5 +/- 10.3 mlU/ml hCG; 10.55 +/- 0.6 vs. 5.3 +/- 0.8 mIU FSH, respectively). There was no correlation between early embryonic growth rate and FF concentrations of FSH, hCG, and PRL. Ova reaching the two-cell stage 40 h after fertilization in vitro were associated with the same FF concentrations of FSH, hCG, and PRL as those that cleaved to the four-cell stage. The PRL concentration in FF was significantly higher in mature fertilized ova and in fertilized ova that were associated with a successful pregnancy. It is suggested that the intrafollicular concentration of FSH is associated with the degree of mucification of the OCCC, but FF levels of both FSH and hCG are associated with successful fertilization. High levels of PRL in FF were associated with successful pregnancy and may imply a role of this hormone in oocyte maturation.

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Section: Discussioncontrasting

confidence: 68%

Gonadotropin and Prolactin Levels in Follicular Fluid of Human Ova Successfully Fertilized in Vitro

Laufer

Botero-Ruiz

DeCherney

et al. 1984

The Journal of Clinical Endocrinology & Metabolism

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“…This finding is in apparent contradiction to the suggestion that prolactin plays a role in inhibiting oocyte maturation (Baker and Hunter 1978). It is also at odds with a recent review; McNeilly (1984) concluded that evidence from a number of mammalian species indicated a role for prolactin at the ovarian level in influencing follicular growth and development.…”

Section: Discussioncontrasting

confidence: 54%

Effect of Suppressing Prolactin in the Mouse on Liveweight, Food Intake and Ovulation Rate

Brien¹

1986

Aust. Jnl. Of Bio. Sci.

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The involvement, if any, of prolactin in the relationship between appetite and ovulation rate was studied in mice. Injections of 0, 50, 100 or 150 p.g of bromocryptine were given twice-daily to 46-day-old virgin mice for a minimum of 15 days. Between days 5 and 12 of treatment, mice receiving either 50, 100 or 150 p.g of bromocryptine consumed 3 '1, 4·3 and 6·2 g more food, respectively, than did mice in the control group. Liveweights and liveweight gain, however, were unaffected by bromocryptine injections. From day 0 to 12 of treatment mice grew 0,16, 0'15, 0·21 and 0·16 g/day in the 0,50, 100 and 150 p.g bromocryptine groups, respectively, (P > O' 05). Plasma prolactin concentrations were suppressed, but ovulation rates were similar in the 50, 100 and 150 p.g bromocryptine groups compared with the control (median prolactin concentrations and mean ovulation rates were 32'9, 32'5 and 31·6 ng/ml and 14'4, 15·1 and 15·7 ova, respectively, compared with 217·2 ng/ml and 14·9 ova in the control).The results do not support the hypothesis that prolactin directly mediates a relationship between appetite and ovulation rate in the post-pubertal mouse.

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“…PRL may stimulate the formation of an oocyte maturation inhibitor in porcine granulosa cells |11|. Furthermore, treatment of cultured porcine follicles with an anti-PRL serum induces meiotic maturation [12]. These findings suggest that PRL may inhibit the nuclear maturation of oocytes.…”

Section: Discussionmentioning

confidence: 77%

“…PRL may stimulate the formation of an oocyte maturation inhibitor from porcine granulosa cells [11]. and treatment with an anti-PRL serum can induce meiotic maturation in cultured porcine follicles [12]. Therefore, the nuclear maturation of oocytes would appear to be impaired by PRL.…”

Section: Introductionmentioning

confidence: 99%

Effects of Prolactin on Fertilization and Cleavage of Human Oocytes

et al. 1991

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The effects of PRL on fertilization and cleavage of human oocytes and subsequent pregnancy were studied. Forty-five patients (47 cycles) with euprolactinemic normal menstrual cycles undergoing in vitro fertilization (IVF) for the treatment of tubal infertility were selected for this study. The patients were divided into three groups dependent upon their mean serum PRL concentrations for the 3 days prior to Oocyte retrieval; hypoprolactinemic ( < 10 µg/I), euprolactinemic (10–30 µg/l) and hyperprolactinemic cycles (≧30 µg/I). Multiple follicular development was induced with hMG, and 10 patients were randomized to receive bromocriptine beginning with the previous menstrual cycle. In the hypoprolactinemic cycle group the fertilization rate was significantly lower than in the hyperprolactinemic cycle group, and the cleavage rate was significantly lower than in the other groups. The fertilization rates and the cleavage rates in the hyperprolactinemic cycle group were higher than those in the euprolactinemic cycle group; however, these differences were not statistically significant. While the pregnancy rates in the euprolactinemic cycle group were higher than in the other two groups, the numbers were too small for meaningful statistical comparison. The present study demonstrates that below normal concentrations of PRL have deleterious effects on IVF outcome. These data suggest that PRL may play a beneficial stimulatory role in Oocyte maturation and the acquisition of developmental capacity.

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Interrelationships between the oocyte and somatic cells within the Graafian follicle of mammals

Abstract: Introduction.

Cited by 24 publications

References 15 publications

Gonadotropin and Prolactin Levels in Follicular Fluid of Human Ova Successfully Fertilized in Vitro

Gonadotropin and Prolactin Levels in Follicular Fluid of Human Ova Successfully Fertilized in Vitro

Effect of Suppressing Prolactin in the Mouse on Liveweight, Food Intake and Ovulation Rate

Effects of Prolactin on Fertilization and Cleavage of Human Oocytes

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