Interrelationships in trace-element metabolism in metal toxicities in nickel-resistant strains of <i>Neurospora crassa</i>

Mohan, P. Maruthi; Sastry, K. Subramanya

doi:10.1042/bj2120205

Cited by 35 publications

(7 citation statements)

References 34 publications

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“…Metal ion antagonisms are well recognized in N. crassa in which toxic metal ion transport and toxicity (cobalt, nickel, and zinc) are inhibited by Mg 2+ (Venkateswerlu and Sastry 1979;Maruthi Mohan and Sastry 1983). However, antagonism with calcium in the same has not been observed.…”

Section: Discussionmentioning

confidence: 94%

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Cell-wall-bound metal ions are not taken up inNeurospora crassa

Latha¹,

Rashmi²,

Mohan³

2005

Can. J. Microbiol.

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To establish the relevance of the cell wall in metal ion transport, cobalt uptake was examined in Neurospora crassa. Cobalt taken up was largely surface bound (>90%), resulting in a release of calcium and magnesium. Surface-bound cobalt could not enter intracellular locations upon further incubation of mycelia in a metal-free medium. Saturation of the surface with one metal augured subsequent dose-dependent entry of a different metal into intracellular locations. In comparison with the cobalt-resistant mutant, the cobalt-sensitive strain of N. crassa bound less cobalt on the surface but with significant intracellular accumulation. Our results demonstrate the importance of the cell wall in metal transport, toxicity, and resistance in fungi.

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Section: Discussionmentioning

confidence: 94%

“…Media composition and growth conditions for N. crassa and other fungi and metal ion estimation procedures were similar to the methods described in earlier studies (Maruthi Mohan and Sastry 1983;Sajani and Maruthi Mohan 1997). Neurospora crassa was grown in 10 mL of medium in 50 mL conical flasks for 72 h at 28 ± 1°C.…”

Section: Media and Growth Conditionsmentioning

confidence: 99%

Cell-wall-bound metal ions are not taken up inNeurospora crassa

Latha¹,

Rashmi²,

Mohan³

2005

Can. J. Microbiol.

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“…Plasmidbound resistance to mercury was extensively reviewed and leads essentially to detoxication by reduction of Hg2+ to Hgo (31). Cobalt and nickel are chemically related; both are transported into cell by the magnesium system (1,5,16,23,27,38), and both act toxically in having iron metabolism as their target in the case of fungi (2,18). So cobA and the nickel resistance might be linked.…”

Section: Discussionmentioning

confidence: 99%

Alcaligenes eutrophus CH34 is a facultative chemolithotroph with plasmid-bound resistance to heavy metals

Mergeay¹,

Nies²,

Schlegel³

et al. 1985

J Bacteriol

746

372

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Akaligenes eutrophus strain CH34, which was isolated as a bacterium resistant to cobalt, zinc, and cadmium ions, shares with A. eutrophus strain H16 the ability to grow lithoautotrophically on molecular hydrogen, to form a cytoplasmic NAD-reducing and a membrane-bound hydrogenase, and most metabolic attributes; however, it does not grow on fructose. Strain CH34 contains two plasmids, pMOL28 (163 kilobases) specifying nickel, mercury, and cobalt resistance and pMOL30 (238 kilobases) specifying zinc, cadmium, mercury, and cobalt resistance. The plasmids are self-transmissible in homologous matings, but at low frequencies. The transfer frequency was strongly increased with IncPl plasmids RP4 and pUZ8 as helper plasmids. The phenotypes of the wild type, cured strains, and transconjugants are characterized by the following MICs (Micromolar) in strains with the indicated phenotypes: Nic+, 2.5; Nic-, 0.6; Cob+A, 5.0; Cob+B, 20.0; Cob-, < 9.07; Zin+, 12.0; Zin-, 0.6; Cad', 2.5; and Cad-, 0.6. Plasmid-free cells of strain CH34 are still able to grow lithoautotrophicaily and to form both hydrogenases, indicating that the hydrogenase genes are located on the chromosome, in contrast to the Hox structural genes of strain H16, which are located on the megaplasmid pHG1 (450 kilobases).

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“…The tcu-1, tcu-2 and tcu-3 deletion strains were constructed by the Neurospora Programme Project Grant Consortium (Colot et al, 2006). The wild type and knockout strains of N. crassa were grown in basal medium (BM) for inducing vegetative growth and conidial germination while synthetic crossing media (SCM) was used for sexual growth (Maruthi Mohan and Sastry, 1983;Westergaard and Mitchell, 1947). These strains were grown in liquid or on solid media (2% agar).…”

Section: Strains Media and Growthmentioning

confidence: 99%