Interspecies Interactions Between Streptococcus Mutans and Streptococcus Agalactiae in vitro

Liu, Tingjun; Liu, Jia; Liu, Jianwei; Yang, Ruiqi; Lu, Xianjun; He, Xuesong; Shi, Wenyuan; Guo, Lihong

doi:10.3389/fcimb.2020.00344

Cited by 13 publications

(6 citation statements)

References 73 publications

(96 reference statements)

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“…Based on the HOMINGS assay, S. mutans was detected in the infant saliva in very low numbers, along with S. sanguinis and other taxa of oral Streptococcus species. The results confirmed that S. mutans may be one of the early colonizers in infant saliva and a critical facilitator for establishing a Streptococcus-associated oral polymicrobial community (42). Furthermore, the core genera in the newborn saliva included not only Streptococcus but also Rothia, Gemella, Veillonella, Staphylococcus, Prevotella, and Granulicatella.…”

Section: Discussionsupporting

confidence: 62%

Dynamics of oral microbiome acquisition in healthy infants: A pilot study

Saraithong

Zhang

et al. 2023

Front. Oral. Health

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ObjectivesThe human oral microbiota is one of the most complex bacterial communities in the human body. However, how newborns initially acquire these bacteria remains largely unknown. In this study, we examined the dynamics of oral microbial communities in healthy infants and investigated the influence of the maternal oral microbiota on the acquisition of the infant's oral microbiota. We hypothesized that the infant oral microbial diversity increases with age.MethodsOne hundred and sixteen whole-salivary samples were collected from 32 healthy infants and their biological mothers during postpartum and 9- and 15-month well-infant visits. Bacterial genomic DNA was extracted and sequenced by Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS) methods. The Shannon index was used to measure the microbial diversity of the infant-mother dyads (alpha diversity). The microbial diversity between the mother-infant dyads (beta-diversity) was calculated using the weighted non-phylogenetic Bray-Curtis distance in QIIME 1.9.1. Core microbiome analysis was performed using MicrobiomeAnalyst software. Linear discriminant analysis coupled with effect size analysis was used to identify differentially abundant features between mother and infant dyads.ResultsA total of 6,870,571 16S rRNA reads were generated from paired mother–infant saliva samples. Overall, oral microbial profiles significantly differed between the mother and infant groups (p < 0.001). The diversity of the salivary microbiomes in the infants increased in an age-dependent manner, whereas the core microbiome of the mothers remained relatively stable during the study period. Breastfeeding and gender did not affect the microbial diversity in infants. Moreover, infants had a greater relative abundance of Firmicutes and a lower abundance of Actinobacteria, Bacteroidetes, Fusobacteria, and Proteobacteria than their mothers. The SparCC correlation analysis demonstrated constant changes in infants' oral microbial community network (p < 0.05).ConclusionsThis study provides new evidence that the oral cavities of infants are colonized by a distinct group of bacterial species at birth. The acquisition and diversity of changes in oral microbial composition are dynamic during the first year of an infant's life. Before reaching the second birthday, the composition of the oral microbial community could be more similar to that of their biological mothers.

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Section: Discussionsupporting

confidence: 62%

Dynamics of oral microbiome acquisition in healthy infants: A pilot study

Saraithong

Zhang

et al. 2023

Front. Oral. Health

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“…For instance, this polysaccharide only binds to SspB expressed by Lactococcus lactis, but does not bind to other L. lactis AgI/II family proteins (Back et al, 2015). A recent study showed that the novel intrageneric coadhesion between S. agalactiae and S. mutans is promoted by glucoside transferase B (GtfB) and GtfC (Liu et al, 2020). Another investigation showed that none of the six Lactobacillus strains tested, including clinical isolates from children with caries, could form biofilms in vitro.…”

Section: Bacterial Interactions During Biofilm Formationmentioning

confidence: 99%

The Oral Microbiota: Community Composition, Influencing Factors, Pathogenesis, and Interventions

et al. 2022

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The human oral cavity provides a habitat for oral microbial communities. The complexity of its anatomical structure, its connectivity to the outside, and its moist environment contribute to the complexity and ecological site specificity of the microbiome colonized therein. Complex endogenous and exogenous factors affect the occurrence and development of the oral microbiota, and maintain it in a dynamic balance. The dysbiotic state, in which the microbial composition is altered and the microecological balance between host and microorganisms is disturbed, can lead to oral and even systemic diseases. In this review, we discuss the current research on the composition of the oral microbiota, the factors influencing it, and its relationships with common oral diseases. We focus on the specificity of the microbiota at different niches in the oral cavity, the communities of the oral microbiome, the mycobiome, and the virome within oral biofilms, and interventions targeting oral pathogens associated with disease. With these data, we aim to extend our understanding of oral microorganisms and provide new ideas for the clinical management of infectious oral diseases.

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“…It is also possible that the different response of the two microorganisms studied here may be due to a greater initial cell surface area (at matching CFU/mL) for C. albicans compared to S. mutans. While C. albicans are larger cells at 5–6 µm [ 57 ], S. mutans have an average cell size of 0.5–0.75 µm [ 58 ]. The lack of dependence of the MIC and MBC on the light application mode for S. mutans is likely due in part to the reduced molar absorptivity of curcumin in BHI.…”

Section: Discussionmentioning

confidence: 99%

A Systematic Evaluation of Curcumin Concentrations and Blue Light Parameters towards Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms

Comeau,

Manso

2023

Pharmaceutics

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Dental caries is a highly preventable and costly disease. Unfortunately, the current management strategies are inadequate at reducing the incidence and new minimally invasive strategies are needed. In this study, a systematic evaluation of specific light parameters and aqueous curcumin concentrations for antimicrobial photodynamic therapy (aPDT) was conducted. Aqueous solutions of curcumin were first prepared and evaluated for their light absorbance after applying different ~56 mW/cm2 blue light treatments in a continuous application mode. Next, these same light treatments as well as different application modes were applied to the curcumin solutions and the molar absorptivity coefficient, reactive oxygen species (ROS) release, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) for Streptococcus mutans and the MIC and minimum fungicidal concentration (MFC) for Candida albicans were measured. After up to 1 min of light treatment, the molar absorptivity of curcumin when added to culture media was lower than that for water only; however, at higher energy levels, this difference was not apparent. There was a noted dependence on both ROS type and cariogenic microorganism species on the sensitivity to both blue light treatment and application mode. In conclusion, this study provides new information towards improving the agonistic potential of aPDT associated with curcumin against cariogenic microorganisms.

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Interspecies Interactions Between Streptococcus Mutans and Streptococcus Agalactiae in vitro

Cited by 13 publications

References 73 publications

Dynamics of oral microbiome acquisition in healthy infants: A pilot study

Dynamics of oral microbiome acquisition in healthy infants: A pilot study

The Oral Microbiota: Community Composition, Influencing Factors, Pathogenesis, and Interventions

A Systematic Evaluation of Curcumin Concentrations and Blue Light Parameters towards Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms

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