Interspecific grafting between Gossypium hirsutum, G. barbadense and G. herbaceum lines

Abstract

Seedling grafting could provide additional crop improvement strategies for cotton. However, there existed limited studies on interspecific grafting and approaches. Four different grafting approaches were developed and compared between lines representing three of the four cultivated cotton species G. hirsutum, G. barbadense and G. herbaceum. Grafting approaches of this study focused on the cotyledon node and cotyledon leaves retained on scions, rootstocks, without cotyledon node and cotyledon leaves on scions and rootstocks or halved cotyledon node and single cotyledon leaf on scions and rootstocks. Evaluations of the grafting approaches were made by comparing survival and growth rate during the second and fifth weeks after transplantation, respectively. The formation of any lateral shoots at the grafted sites were studied in two of four grafting approaches in the first and the second year during flowering stage. DNA alterations due to grafting were investigated using microsatellite markers. There were no statistically significant differences between grafts and their control in survival rate and locus specific DNA alteration. Growth rate and lateral shoot formation, on the other hand, were different among grafting types and grafts. We concluded that grafting without cotyledon node and cotyledon leaves on rootstocks, and with cotyledon node but without cotyledon leaves on scions were easy to perform and suitable for interspecific cotton grafting. Results suggested that grafting seedlings and allowing time to heal graft wounds prior to spring transplanting or double cropping is suitable for wheat–cotton intercropping to prevent late or early chilling damage associated with seed sowing or conventional transplanting of susceptible seedlings. Furthermore, the rapid and consistent wound healing in seedling grafts along with lateral shoot formation occurring in two of four grafting approaches make them a suitable approach to investigate possible genetic and epigenetic movement between scions and rootstocks, especially across species.

Vegetative Propagation of Cotton (Gossypium spp.) By Rooting

“…While cotton is propagated by grafting method [1,[6][7], Özyiğit [2] produced vegetative from internodes under in vitro conditions. In addition, Karaca et al [5] had been successful in seedling with grafting. In addition, Karaca et al [5] had been successful in seedling with grafting.…”

“…In addition, Karaca et al [5] had been successful in seedling with grafting. In addition, Karaca et al [5] had been successful in seedling with grafting. In this study, another vegetative reproduction method has been experiment, which rooting.…”

“…Numerous cotton clones have been produced via micro-propagation from shoot [2], cotyledon explants [3] and hypocotyl of cotton seedlings [4]. Moreover, cotton was propagation as vegetative by grafting method in Turkey [1,5]. The aim of this work is to search possibility of vegetative propagation on cotton (Gossypium spp) by rooting.…”

A Guide to Grafting for Cotton (Gossypium Hirsutum L.) Virus Transmission and the Successful Transmission of Cotton Leaf Roll Dwarf Virus

“…Grafts used successfully in cotton include wedge, modified bottle shoot, and T-graft. (Karaca et al 2020;Reddall et al 2004;Ahktar et al 2002;Sharman, Personal Communication). The literature is limited on why particular grafts were chosen for cotton virus transmission work (Table 1.).…”

Effects of Interspecific Grafting Between Capsicum Species on Scion Fruit Quality Characteristics

“…The reconnection of vascular continuity between different rootstock and scion genotypes, compounded by the intermediate formation of callus, may alter the flow rate of metabolites across the graft union, resulting in changes in scion morphology and fruit characteristics (Berger et al, 2018;Davis et al, 2008). Moreover, grafting may trigger epigenetic changes that alter the transcription of genes in the scion (Li et al, 2021;Karaca et al, 2020). In peppers, desirable and often undesirable changes in fruit shape, size, and weight have been observed after grafting (Doñas-Ucl es et al, 2015;Kyriacou et al, 2017).…”