Interspecific hybridization between Ganoderma lingzhi and G. applanatum through protoplast fusion

Raman, Jegadeesh; Jang, Kab-Yeul; Oh, Youngjae; Oh, Minji; Im, Ji-Hoon; Lakshmanan, Hariprasath; Kong, Won-Sik

doi:10.1007/s11274-021-03084-5

Cited by 12 publications

(10 citation statements)

References 54 publications

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“…The two hybrid strains with different dominant characteristics (WR and RW) also yielded higher sugar content than the two natural strains of S. commune (W and R). These results are consistent with those of other studies and suggest that the increase of total sugar contents of the hybrid strains is most likely related to the changes in the genetic structure leading to an overexpression of genes encoding information of polysaccharides synthesis (Liu et al, 2017;Raman et al, 2021).…”

Section: Chemical Compositions and Antioxidant Activities Of Crude Ho...supporting

confidence: 92%

“…Furthermore, the exchange of genes between two nuclei during hybridisation may produce hybrids with diverse genetic materials and this may also result in differential expression of genes between the hybrid strains compared to the parental strains. According to Raman et al (2021), the genetic profile of ten hybrids was detected using inter-simple sequence repeats (ISSR) and RAPD markers, the results depicted those hybrids contain polymorphic bands indicating the rearrangement and deletion in genetic structure. Moreover, Liu et al (2017) reported that certain hybrids have higher concentrations of polysaccharides and triterpenes than that of parental strains.…”

Section: Chemical Compositions and Antioxidant Activities Of Crude Ho...mentioning

confidence: 99%

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Antioxidant activities and chemical composition of hot water-soluble extracts and fractions of selected Schizophyllum commune Fr. strains

Al-Salihi¹,

Lau²

2022

ijhs

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Oxidative stress is a conjoint pathological mechanism that can be triggered by free radicals leading to cellular senescence or apoptosis. Antioxidants have the potential to inhibit free radicals’ generation and balance the irregulated oxidative stress to restore cellular homeostasis. Schizophyllum commune, also known as the split-gill mushroom, is well-known for its nutritional values and bioactive compounds. This study aimed to investigate the antioxidant activity and chemical composition of the hot water extracts of selected strains of S. commune including natural, gamma-irradiated and hybrid strains. The fruiting bodies of different strains of S. commune were subjected to hot water extraction and the extracts were evaluated by radical scavenging, and reducing capacity assays. Results showed that, overall, the antioxidant activity of the various strains was comparable. The natural strain W and the strain obtained when strain W was subjected to gamma-irradiation at a dose of 4000 Gy (W4000) consistently exhibited high antioxidant activities in all assays and they were either comparable or higher than common edible mushrooms, Pleurotus pulmonarius and Agaricus bisporus. Moreover, the gamma-irradiated strains R2000 and R4000 exhibited higher reducing activities (0.40 and 0.41 mmol Fe2+/g extract) than the parental strain R (0.27 mmol Fe2+/g extract).

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Section: Chemical Compositions and Antioxidant Activities Of Crude Ho...supporting

confidence: 92%

Section: Chemical Compositions and Antioxidant Activities Of Crude Ho...mentioning

confidence: 99%

Antioxidant activities and chemical composition of hot water-soluble extracts and fractions of selected Schizophyllum commune Fr. strains

Al-Salihi¹,

Lau²

2022

ijhs

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“…Osmotic stabilizers, including inorganic salts, sugars, and sugar alcohols, were used to stabilize protoplasts isolated from mycelium. The concentration, molarity, and the type of stabilizer can impact the yield of the protoplast [26,35]. Depending on their lifestyle, lamentous fungi obtain their nutrients from the environment.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of pigment and proximate values of intergeneric hybrid of protoplast fusion of Monascus ruber and Pleurotus ostreatus

Kannan

Nanthini

2022

Preprint

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Protoplast fusion is a crucial technique for strain enhancement that brings genetic recombination to filamentous fungus and creates hybrid strains. In this present study, the intergenic fusion between M. ruber and P. ostreatus has been carried out to enhance pigments, secondary metabolites, and proximate values. Protoplasts from M. ruber and P. ostreatus were isolated and an equal amount of protoplast was taken and performed protoplast fusion. The regenerated colonies were plated in potato dextrose agar with selected biological markers (nystatin and fluconazole) for the confirmation of the hybrid. In a standardized condition, the protoplast yield of M. ruber and P. ostreatus was 2.31×107 cells/ml and 2.33×107 cells/ml, respectively. Acidic pH yielded high amounts of protoplasts than neutral and alkaline pH. KCl showed a high yield of protoplasts 2.36×106 cells /ml from M. ruber and 2.46×107cells/ml from P. ostreatus. The colony resistance to nystatin and fluconazole was confirmed that the obtained regenerated colonies were fusant. The fusant has no septation in its transverse walls with lipid droplets. Fusion of protoplast from M. ruber and P. ostreatus resulted in genetic inference at the metabolic levels, explaining the elevation of pigment in fusant colonies. The proximate composition of fusant strains was higher. In conclusion, we have developed a new strain with genetic variance capable of producing an increased level of pigment with nutritional value through protoplast fusion that allows the generation of fusant with the characteristics of parent strains and elucidates the effectiveness of fusant for commercial applications.

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“…To release MOEVs into the solution, plant cell walls were degraded with enzymes following a modified protocol for plant protoplast preparation 24 – 27 . Briefly, fresh roots of MO were cut into 1 mm pieces using a special slicer for Chinese medicine before enzyme digestion to increase the surface area and accelerate the rate of enzyme degradation of the cell wall.…”

Section: Methodsmentioning

confidence: 99%

An enzyme-based system for extraction of small extracellular vesicles from plants

Zhao,

Liu,

Liu

et al. 2023

Sci Rep

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Plant-derived nanovesicles (NVs) and extracellular vesicles (EVs) are the next generation of nanocarrier platforms for biotherapeutics and drug delivery. EVs exist not only in the extracellular space, but also within the cell wall. Due to the limitations of existing isolation methods, the EVs extraction efficiency is low, and a large amount of plant material is wasted, which is of concern for rare and expensive medicinal plants. We proposed and validated a novel method for isolation of plant EVs by enzyme degradation of the plant cell wall to release the EVs. The released EVs can easily be collected. The new method was used for extraction of EVs from the roots of Morinda officinalis (MOEVs). For comparison, nanoparticles from the roots (MONVs) were extracted using the grinding method. The new method yielded a greater amount of MOEVs, and the vesicles had a smaller diameter compared to MONVs. Both MOEVs and MONVs were readily absorbed by endothelial cells without cytotoxic effect and promoted the expression of miR-155. The promotion of miR-155 by MOEVs was dose-dependent. More importantly, we found that MOEVs and MONVs were enriched toward bone tissue. These results support our hypothesis that EVs in plants could be efficiently extracted by enzymatic cell wall digestion and confirm the potential of MOEVs as therapeutic agents and drug carriers.

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Interspecific hybridization between Ganoderma lingzhi and G. applanatum through protoplast fusion

Cited by 12 publications

References 54 publications

Antioxidant activities and chemical composition of hot water-soluble extracts and fractions of selected Schizophyllum commune Fr. strains

Antioxidant activities and chemical composition of hot water-soluble extracts and fractions of selected Schizophyllum commune Fr. strains

Enhancement of pigment and proximate values of intergeneric hybrid of protoplast fusion of Monascus ruber and Pleurotus ostreatus

An enzyme-based system for extraction of small extracellular vesicles from plants

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