Interspecific hybridization between Tigridia pavonia and T. augusta through ovary slice culture

Abstract

Tigridia pavonia is the most popular species in the Tigridia genus, and is currently marketed in Europe, Asia, and Australia as a landscape plant. Although it is native to Mexico, there are no breeding programs for it. In this study, we attempted to increase its flower color spectrum and growth habit by interspecific hybridization with T. augusta. Interspecific hybrids between T. pavonia and T. augusta were successfully obtained for the first time using the cut-style pollination and ovary slice culture techniques. On the contrary, no hybrids were obtained from a reciprocal cross. At three, four, and five days after pollination (DAP) ovaries were sliced and cultured on Murashige and Skoog medium without growth regulators and ammonium nitrate, but were supplemented with 6% sucrose, 50 mg/L yeast extract, and 0.25% Gelrite. After 80 days of culture initiation, the germination of only 10 embryos was observed in ovary slices cultured at three DAP. After transfer to identical fresh medium, six hybrid embryos developed into seedlings. All obtained hybrid seedlings were transplanted successfully to soil, and grew normally. The progenies investigated were identified as true hybrids based on randomly amplified polymorphic DNA analysis.

CHARACTERIZATION OF THE SENESCENCE-ASSOCIATED PROTEASES OF THE “TIGER FLOWER” (Tigridia pavonia) L.F. DC

“…Tigridia pavonia (L.f) DC also known as "Oceloxochitl" or "Tiger flower" is a Mexican native ornamental plant that displays great variability in color and beauty of its ephemeral flowers (Piña-Escutia et al, 2013;Arzate-Fernández et al, 2015;Arroyo-Martínez et al, 2017). Although their flower longevity consists of one day, flower production continues for up eight weeks, being an excellent outdoor plant.…”